On-line CE (capillary electrophoresis) detection device

Abstract

The invention discloses an on-line CE (capillary electrophoresis) detection device and belongs to the technical field of an electrophoresis method. The detection device comprises a light source for producing optical wavelengths for detection, a first optical system, a capillary tube for storing a sample to be separated, an electrophoresis bracket, a second optical system and an image sensor, wherein the capillary tube is positioned on the electrophoresis bracket, the light source and the first optical system are arranged on one side of the capillary tube, the second optical system and the image sensor connected with the second optical system are arranged on the other side of the capillary tube, the first optical system shapes light wave fields produced by the light source to serve as incidence light waves for detection of protein or macromolecules in the capillary tube, the second optical system maps images of the capillary tube as well as the separated macromolecules or protein in the capillary tube to the image sensor, and the image sensor detects the images and converts the images into electric signals. According to the on-line CE detection device, the macromolecules and the protein can be separated online in a gel electrophoresis manner, the relative content of the separated protein and macromolecules can be detected rapidly and simultaneously, the test procedure is simplified, and the detection device is rapid and accurate.

Description

technical field [0001] The invention relates to a device in the technical field of electrophoresis, in particular to an online capillary electrophoresis and detection device. Background technique [0002] Capillary electrophoresis (Capillary electrophoresis, CE) is a method for efficient and rapid separation of ions or charged ions (such as macromolecular proteins) in capillaries driven by an external electric field according to the difference in their mobility / partition coefficient. As a micro-separation analysis technology, CE combines the advantages of traditional electrophoresis technology and micro-column separation technology, and has the characteristics of high resolution, high sensitivity, fast high-throughput and low sample consumption. CE has been widely used in the field of biochemical analysis in recent years. [0003] After searching the prior art, it is found that Chinese Patent Document No. CN102879361, published on 2013-01-16, records a detection system. Ca...

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Problems solved by technology

However, the defect and deficiency of this prior art compared with the present invention is that this technology adopts a time-resolved detection technology, which uses three separate individual detection units, and can only resolve the electrophoretic signal of one spatial position at the same time

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