A kind of micro-nano array sensor and its preparation method

Abstract

The invention discloses a micro-nano annular electrode sensor for quantitatively determining single cells in situ and releasing substances, and a preparation method of the micro-nano annular electrode sensor. The micro-nano annular electrode sensor comprises an annular micro-nano electrode array, a fixed-point nano modified layer, a specific election reaction modified layer and a selective cell adsorption layer, wherein the annular micro-nano electrode array is an array composed of annular micro-nano electrodes; the fixed-point nano modified layer is located on the inner surfaces of the annular micro-nano electrodes and is used for enhancing the electrode performances; the specific election reaction modified layer is formed by fixing different specific materials on the surface of the fixed-point nano modified layer and is used for correspondingly detecting different neurotransmitters of the different cells; the selective cell adsorption layer is formed on the surface of the specific election reaction modified layer and is used for selectively adsorbing the cells. The annular micro-nano electrode sensor disclosed by the invention is the annular micro-nano electrode sensor which is small in volume, has a plurality of recording points, has no damages to nerve cells and can be used for simultaneously detecting the plurality of recording points on a two-dimensional scale (a plurality of cells and various neurotransmitters can be detected simultaneously).

Description

technical field [0001] The invention relates to the technical field of micro-nano preparation of biosensors and electroanalytical chemistry, in particular to a micro-nano array sensor capable of in situ detection of single-cell electrophysiology and various electrochemical neurotransmitter signals and a preparation method thereof. Background technique [0002] The transmission of information between nerve cells occurs through chemical neurotransmitters and action potentials inside neurons. The release of neurotransmitters in nerve cells regulates the normal progress of life activities. Real-time recording of quantized neurotransmitter release from nerve cells is an important and direct way to understand nerve signal transduction and mechanism of action. Abnormal cell release will lead to biological dysfunction and various diseases, such as senile dementia, cardiovascular and cancer. Therefore, neurotransmitter proton release is of great significance in the fields of cellul...

AI Technical Summary

Problems solved by technology

With the development of microelectromechanical system (MEMS) processing technology, microelectrode array (microelectrode, MEA) provides a long-term, multi-site monitoring method for cells. The diameter of conventional MEA electrodes is 10-50 μm, which is much larger than that of vesicles and vesicles. Although the quantum release size of the synapse can be localized to a single cell, the spatial and temporal resolution is still insufficient for the quantum release of the detection of synaptic vesicles, and the spatial difference of the release at different positions of the cell on a single electrode cannot be distinguished , it is difficult to detect the real-time quantum release signal

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