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Method for detecting drug resistance of adelphocoris suturalis

A kind of black stink bug, drug-resistant technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of no improvement, cumbersome operation, shortening the time of inspection results, etc., to reduce cumbersome steps and solve the problem of large amount of insects , Rapid detection convenient and reliable effect

Inactive Publication Date: 2014-03-26
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Toxicity determination is a common method for indoor understanding of pest resistance. However, the traditional leaf drug film method and spot method are cumbersome to measure toxicity. Several concentrations must be set and repeated to ensure the reliability of the results.
The glass tube drug film method is a new method for rapid detection of pest resistance. Due to its obvious effect, it shortens the time for checking results, but it is still the same as the traditional method in terms of concentration and repetition.

Method used

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  • Method for detecting drug resistance of adelphocoris suturalis
  • Method for detecting drug resistance of adelphocoris suturalis
  • Method for detecting drug resistance of adelphocoris suturalis

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Obtaining of embodiment 1 diagnostic dosage

[0025] 1. Research object

[0026] The research object of the present invention is the susceptible population of Lygus melanogaster raised in the laboratory and not exposed to any medicament.

[0027] 2. Method

[0028] The test agents were imidacloprid, endosulfan, cyhalothrin and chlorpyrifos, and the toxicity was determined by glass tube film method.

[0029] 2.1 Configuration and dilution of the mother liquor of the original drug

[0030] Dissolve the original drug in acetone, put it in a medicine bottle after constant volume, seal it with parafilm, and store it in a refrigerator at 4°C. Before the toxicity test, the original drug mother solution was diluted to various concentrations with acetone and set aside.

[0031] 2.2 Preparation of glass tube drug film

[0032] Glass tubes used in experiments (such as figure 1 shown) must undergo rigorous washing to ensure that no drug remains. Draw 220μL from the diluted d...

Embodiment 2

[0038] Example 2 Diagnosis Dose Detection of Antimicrobial Resistance of Lygus chinensis

[0039] 1. Research object

[0040] The research object of this embodiment is the population of Lygus melanogaster to be tested in the field.

[0041] 2. Method

[0042] The tested medicaments were imidacloprid, endosulfan, cyhalothrin and chlorpyrifos, and the diagnostic dose of the present invention was used to make a glass tube drug film, and the resistance level of the Lygus stinkbug was detected in the field.

[0043] 2.1 Configuration and dilution of the mother liquor of the original drug

[0044] The original drug is dissolved in acetone, and the mother liquor of the original drug is diluted with the diagnostic dose obtained in Example 1 with acetone for subsequent use.

[0045] 2.2 Preparation of glass tube drug film

[0046] Glass tubes used in experiments (such as figure 1 shown) must undergo rigorous washing to ensure that no drug remains. Draw 220μL from the diluted drug...

Embodiment 3

[0050] Example 3 The traditional glass tube drug film method is used to measure the drug resistance of the black Lygus population in the field

[0051] 1. Research object

[0052]The object of this case study is the tested population of Lygus stinkbug in the field.

[0053] 2. Method

[0054] The test agents were imidacloprid, endosulfan, cyhalothrin and chlorpyrifos, and the toxicity was determined by glass tube film method.

[0055] 2.1 Configuration and dilution of the mother liquor of the original drug

[0056] Dissolve the original drug in acetone, put it in a medicine bottle after constant volume, seal it with parafilm, and store it in a refrigerator at 4°C.

[0057] Before the toxicity test, dilute the mother liquor of the original drug to 5-7 concentrations with acetone, and set aside.

[0058] 2.2 Preparation of glass tube drug film

[0059] Glass tubes used in experiments (such as figure 1 shown) must undergo rigorous washing to ensure that no drug remains. Dr...

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Abstract

The invention provides a method for detecting the drug resistance of adelphocoris suturalis. A diagnosis glass tube is manufactured under the diagnostic doses of medicaments so as to rapidly detect the resistance level to pesticides by the adelphocoris suturalis field population, wherein the medicaments are imidacloprid, endosulfan, cyfluthrin or chlorpyrifos; the diagnostic doses of the medicaments are 824ng / cm<2> of imidacloprid, 20ng / cm<2> of endosulfan, 861ng / cm<2> of cyfluthrin and 7ng / cm<2> of chlorpyrifos. The method provided by the invention is simple and convenient to use; the result is intuitive and obvious; the method is suitable for rapidly detecting the resistance level of adelphocoris suturalis in fields, can be used for overcoming the defects of high sample using quantity, complicity in operation, time consumption and the like by a traditional bioassay method, and can be applied to rapidly detecting the resistance level of the adelphocoris suturalis field population.

Description

technical field [0001] The invention relates to a method for measuring the pesticide resistance of agricultural pests, in particular to a method for detecting the pesticide resistance of the black bug. Background technique [0002] In recent years, with the large-scale planting of transgenic Bt cotton in my country, cotton bollworm has been effectively controlled. However, due to the reduction in the use of chemical agents in cotton fields, miridids have risen to become the main pest. At the same time, due to the adjustment of agricultural industry structure, fruit trees The planting area of ​​vegetables and vegetables has increased substantially, providing rich host plants and suitable wintering places for the mirid bug, and the occurrence and damage are becoming more and more serious. The host range of Miridae is very wide, with 147 species in 38 families, among which there are Malvaceae, Leguminosae, Compositae, Cruciferae and many kinds of fruit forest trees, etc., which ...

Claims

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Application Information

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IPC IPC(8): G01N33/00A01K67/033
Inventor 高希武刘晓岚谭瑶许新新冯晶晶甄从爱张帅
Owner CHINA AGRI UNIV
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