Preparation method of pig spleen transfer factor

A porcine spleen transfer factor and spleen technology, which can be used in medical formulas, medical preparations containing active ingredients, unknown raw materials, etc., can solve the problems of incomplete cell fragmentation, high operating costs, and long production cycles, etc., to enhance animal immunity function, maintain the activity of transfer factor, and shorten the production cycle

Inactive Publication Date: 2014-04-09
TIANJIN RINGPU BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] It can be found from the above technical solutions of the prior art that the dialysis method refers to performing several times of dialysis on the supernatant of the homogenate of the animal spleen to realize the separation and purification of the transfer factor. This method is simple to operate, but the production cycle is long and the operation cost is high. , it is difficult to carry out large-scale industrial production; the filtration method is to crus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1) Remove about 65.0kg of frozen pig spleen from surface packaging, and then thaw it with purified water overnight;

[0027] 2) Wash the spleen with purified water on the next day, pick out unqualified spleens, and remove large tissues such as fascia and fat;

[0028] 3) Weigh 6.0kg / portion of spleen, mince it with a meat grinder, and coarsely mince it twice;

[0029] 4) Add 0.1 times the mass (1.2kg) of purified water to every 2 parts, mix well, grind 2 times by oblique tooth colloid mill, and grind 1 time by diamond tooth colloid mill, put into clean container, and pack together 5 barrels; the remaining spleen is recovered and frozen for storage;

[0030] 5) Add 1.3 times the mass (15.6kg) of purified water to each barrel and stir to make a homogenate;

[0031] 6) The homogenate is broken through a high-pressure homogenizer, homogenized once, and the temperature of the slurry is controlled online at 35-40°C;

[0032] 7) All the slurry after breaking the wall is injected into th...

Embodiment 2

[0037] 1) Remove about 65.0kg of frozen pig spleen from surface packaging, and then thaw it with purified water overnight;

[0038] 2) Wash the spleen with purified water on the next day, pick out unqualified spleens, and remove large tissues such as fascia and fat;

[0039] 3) Weigh 6.0kg / portion of spleen, mince it with a meat grinder, and coarsely mince it twice;

[0040] 4) Add 0.25 times the mass (3.0kg) of purified water to every 2 parts, mix well, and then grind 2 times with an oblique tooth colloid mill and 1 time with a diamond-shaped tooth colloid mill, put them in a clean container, and pack them together. 5 barrels; the remaining spleen is recovered and frozen for storage;

[0041] 5) Add 1.25 times the mass of purified water (15.0kg) to each barrel and stir to make a homogenate;

[0042] 6) The homogenate is broken through a high-pressure homogenizer, and the homogenization is carried out twice, and the temperature of the slurry is controlled online at 30~35℃;

[0043] 7) T...

Embodiment 3

[0048] 1) Remove about 65.0kg of frozen pig spleen from surface packaging, and then thaw it with purified water overnight;

[0049] 2) Wash the spleen with purified water on the next day, pick out unqualified spleens, and remove large tissues such as fascia and fat;

[0050] 3) Weigh 6.0kg / portion of spleen, mince it with a meat grinder, and coarsely mince it 3 times;

[0051] 4) Add 0.5 times the mass (6.0 kg) of purified water to every 2 parts, mix well, grind 2 times with an oblique tooth colloid mill and 1 time with a diamond tooth colloid mill, put them in a clean container, and divide them. 5 barrels; the remaining spleen is recovered and frozen for storage;

[0052] 5) Add 1.1 times the mass (13.2kg) of purified water to each barrel and stir to make a homogenate;

[0053] 6) The homogenate is broken through the high-pressure homogenizer, and the homogenization is performed 3 times, and the temperature of the slurry is controlled online at 25-30℃;

[0054] 7) The slurry after brea...

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PUM

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Abstract

The invention provides a preparation method of a pig spleen transfer factor. The preparation method comprises the steps of tissue mincing, breaking by homogenizing, microfiltration, ultrafiltration and the like. A high-pressure homogenizer is adopted to rapidly break cells and can effectively keep the activity of the transfer factor; besides, compared with the prior art, the technical scheme provided by the invention does not need a freezing-thawing step, therefore, the production period is shortened; the microfiltration-ultrafiltration technology is adopted to intercept effective constituents with appropriate molecular weight, so as to obtain a transfer factor stock solution, and compared with the traditional dialysis technology, the microfiltration-ultrafiltration technology is relatively high in efficiency. The method provided by the invention is suitable for scale production requirement, and the production period can be greatly shortened; the transfer factor solution prepared from the healthy pig spleen as a raw material through defatting, cell breakage, microfiltration and ultrafiltration is non-specific immunoregulator and is used for enhancing the immunological function of animal bodies.

Description

Technical field [0001] The invention relates to the field of veterinary biological products, in particular to a method for preparing pig spleen transfer factor. Background technique [0002] Transfer factor (TF) is a substance released by lymphocytes that can transfer sensitization information. It can specifically transfer the donor’s cellular immune information to the recipient, thereby enhancing the recipient’s immune function. It has become a widely used immune enhancement agent abroad. [0003] The transfer factor exists in the lymphocytes of the spleen. The transfer factor preparation method in the prior art generally involves crushing the animal spleen, destroying the cell structure to release the intracellular transfer factor into the serum, and then separating and purifying the transfer factor in the serum. Finally, the transfer factor product is obtained. [0004] The composition of animal spleen slurry is complex, so animal TF is not easy to separate and purify; the molec...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61P37/04A61K35/26
Inventor 王甜苏建东王尚尚杨燚田凤菊夏广欣李建丽吴全忠杨保收李守军
Owner TIANJIN RINGPU BIO TECH
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