A kind of method for increasing roots of jatropha curcas regenerated seedlings

Abstract

The invention belongs to the technical field of vegetative propagation, and provides a method for increasing a root of a barbadosnut regenerated plant. The method comprises the following steps and conditions: (1) firstly, spreading a layer of sterilized substrate material at the bottom of a tissue culture vessel, and then adding a root medium, sterilizing and drying until no water drop exists on the surface of the root medium; (2) inoculating the barbadosnut regenerated plant into the root medium, wherein the root of the regenerated plant is arranged at the connected part of the root medium and the substrate material and does not contact the tissue culture vessel; and then culturing the root until the barbadosnut root extends by attaching to the bottom of the tissue culture vessel under the conditions that the illumination intensity is 1500-2000Lx and the illumination time is 12-16h / d so as to finish root increase of the barbadosnut regenerated plant. According to the method, not only are stability and efficiency are achieved, but also the cost is low, and the transplanting survival rate of the regenerated plant and the growth of the transplanted regenerated plant can be greatly improved.

Description

technical field [0001] The invention belongs to the technical field of plant asexual reproduction, and in particular relates to a method for increasing roots of regenerated seedlings of Jatropha curcas. Background technique [0002] Jatropha ( Jatropha curcas L.) is a plant of the Euphorbiaceae Jatropha genus, which is a deciduous shrub or small tree. The oil content of its seed kernel can be as high as 40% to 60%, which can be used in diesel engines. Jatropha curcas is the most potential for the development of biodiesel One of the energy plants. Jatropha curcas contains active ingredients such as anti-virus and anti-tumor. At present, there are toxicological test studies and preliminary studies on animal models of anti-virus and anti-tumor active ingredients in Jatropha curcas. In addition, Jatropha curcas also has practical application value in the fields of pesticides, silkworm products and daily chemicals. However, the limited distribution of Jatropha curcas, the low ...

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Problems solved by technology

At present, the leaves, cotyledons, epicotyls, hypocotyls and axillary buds of Jatropha curcas can be used as explants to obtain Jatropha curcas regenerated seedlings, but Jatropha curcas regenerated seedlings have problems such as small number of roots and weak roots in the rooting stage. Cause the transplanting survival rate of Jatropha curcas to be low, after transplanting, growth is slow, growing way is poor, is difficult to obtain the Jatropha curcas plant that has practical application value

In addition, the regenerated roots of Jatropha curcas are grown in agar when the medium is used for rooting culture. Since the agar is very easy to be infected with bacteria, the roots of the regenerated seedlings are easy to rot or even die after transplanting. Therefore, before transplanting the regenerated seedlings, The agar remaining on the roots must be cleaned, but cleaning the agar attached to the roots is likely to cause damage to the root system of the regenerated seedlings, and will also affect the transplanting survival rate of the regenerated seedlings and the growth of the regenerated seedlings after transplanting

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