Check patentability & draft patents in minutes with Patsnap Eureka AI!

Method for detecting phadodendrol in cosmetics

A technique for rhododendrol and cosmetics, applied in the field of cosmetic analysis, can solve the problem of rhododendrol detection being in a blank state and the like, and achieve the effects of simple and effective sample pretreatment, avoiding peak broadening and accurate detection results.

Inactive Publication Date: 2014-12-31
苏州出入境检验检疫局检验检疫综合技术中心
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the reports on rhododendol in the literature at home and abroad mainly focus on its occurrence in organisms and its biosynthesis method, etc. There are no reports related to the research on its detection technology, and the detection of rhododendol in cosmetics is still in a blank state

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting phadodendrol in cosmetics
  • Method for detecting phadodendrol in cosmetics
  • Method for detecting phadodendrol in cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1 establishes rhododendron alcohol standard curve

[0041] Dissolve 50 mg rhododendron alcohol (purity ≥ 85%) in acetonitrile to prepare a standard stock solution with a concentration of 1000 μg / mL. Pipette the standard stock solution and prepare standard working solutions with concentrations of 15, 50, 100, 250, 500, and 1000 mg / L with formic acid-acetonitrile containing 0.1% by volume respectively, and take the standard working solutions of each concentration, After filtering through a 0.22 μm filter membrane, take 10 μL of injection samples for determination according to liquid chromatography conditions. Each series of samples is injected 3 times, and the average peak area value is taken.

[0042] Liquid chromatography conditions:

[0043] High-performance liquid chromatography equipped with a fluorescence detector (Agilent 1260, Agilent, USA);

[0044] Chromatographic column: Phenomenex Kinetex C18, 100 × 4.6mm (inner diameter), 2.6 μm (particle size); ...

Embodiment 2

[0052] Example 2 Detection of rhododendron in cosmetics

[0053] (1) Sample handling

[0054] Weigh 0.20 g of the sample to be tested (accurate to 0.001 g), put it in a 10 mL plastic centrifuge tube, add 0.15 mL of 0.02 mol / L ammonium acetate aqueous solution, vortex for 30 s, and add 5% trichloroacetic acid-acetonitrile to mix Extraction (70:30, V / V) 1.85 mL, vortexed for 60 s, ultrasonically extracted for 15 min, then centrifuged at 10,000 rpm for 10 min, the supernatant was passed through a 0.22 μm microporous membrane to obtain the extract solution, Standby; the above-mentioned samples to be tested are respectively cream, lotion, water and facial mask.

[0055] (2) Liquid chromatography conditions

[0056] High-performance liquid chromatography equipped with a fluorescence detector (Agilent 1260, Agilent, USA);

[0057] Chromatographic column: Phenomenex Kinetex C18, 100 × 4.6mm (inner diameter), 2.6 μm (particle size);

[0058] Mobile phase: A: formic acid aqueous sol...

Embodiment 3

[0067] Example 3 Detection of rhododendol in cosmetics with a concentration of 1 mg / kg rhododendron

[0068] (1) Sample handling

[0069] Using the same cream, lotion, lotion and facial mask as the base in Example 2, prepare a test sample of cream, lotion, lotion and facial mask containing rhododendol at a concentration of 1 mg / kg.

[0070] Weigh 0.20 g of the sample to be tested (accurate to 0.001 g), put it in a 10 mL plastic centrifuge tube, add 0.15 mL of 0.02 mol / L ammonium acetate aqueous solution, vortex for 30 s, and add 5% trichloroacetic acid-acetonitrile to mix Extraction (70:30, V / V) 1.85 mL, vortexed for 60 s, ultrasonically extracted for 15 min, then centrifuged at 10,000 rpm for 10 min, the supernatant was passed through a 0.22 μm microporous membrane to obtain the extract solution, spare.

[0071] (2) Liquid chromatography conditions

[0072] High-performance liquid chromatography equipped with a fluorescence detector (Agilent 1260, Agilent, USA);

[0073] ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for detecting phadodendrol in whitening cosmetics through high performance liquid chromatography. The method comprises the following steps: adding ammonium acetate aqueous solution into a to-be-detected sample, then adding 5% trichloroacetic acid-acetonitrile solution and then performing ultrasonic extraction, centrifuging extraction solution, filtering supernatant through a microfiltration membrane and then performing high performance liquid chromatography determination. A fluorescent detection is adopted for detection, the fluorescent excitation and emission wavelengths are respectively 280nm and 315nm. The method for determining the phadodendrol through high performance liquid chromatography is fast, simple and convenient in pretreating, low in detection limit, and high in recovery rate.

Description

technical field [0001] The invention belongs to the field of cosmetic analysis, in particular to a method for detecting rhododendron in cosmetics. Background technique [0002] The chemical name of rhododendron is 4-(4-hydroxyphenyl)-2-butanol. The pure product is white powder, soluble in water and organic solvents such as methanol, ethanol and acetonitrile. Rhododendrol is mainly found in medicinal plants such as birch, aceraceae, fir and turmeric, and is mainly used as a raw material for the biocatalytic synthesis of spices (such as raspberry ketone and betulin); due to its structure and tyrosinase activity inhibitors Similar to most of them having a phenolic structure, rhododendol has the effect of inhibiting the synthesis of melanin, so it is also used as a whitening ingredient in cosmetics. However, it is precisely because of the effect of inhibiting the synthesis of melanin that it interferes with the normal enzymatic conversion process of tyrosine into melanin in the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 徐振东石建华潘葵顾娟红余雯静郑利陈军朱振华
Owner 苏州出入境检验检疫局检验检疫综合技术中心
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com