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Method for detecting phadodendrol in cosmetics

A technique for rhododendrol and cosmetics, applied in the field of cosmetic analysis, can solve the problem of rhododendrol detection being in a blank state and the like, and achieve the effects of simple and effective sample pretreatment, avoiding peak broadening and accurate detection results.

Inactive Publication Date: 2014-12-31
苏州出入境检验检疫局检验检疫综合技术中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the reports on rhododendol in the literature at home and abroad mainly focus on its occurrence in organisms and its biosynthesis method, etc. There are no reports related to the research on its detection technology, and the detection of rhododendol in cosmetics is still in a blank state

Method used

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  • Method for detecting phadodendrol in cosmetics
  • Method for detecting phadodendrol in cosmetics
  • Method for detecting phadodendrol in cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1 establishes rhododendron alcohol standard curve

[0041] Dissolve 50 mg rhododendron alcohol (purity ≥ 85%) in acetonitrile to prepare a standard stock solution with a concentration of 1000 μg / mL. Pipette the standard stock solution and prepare standard working solutions with concentrations of 15, 50, 100, 250, 500, and 1000 mg / L with formic acid-acetonitrile containing 0.1% by volume respectively, and take the standard working solutions of each concentration, After filtering through a 0.22 μm filter membrane, take 10 μL of injection samples for determination according to liquid chromatography conditions. Each series of samples is injected 3 times, and the average peak area value is taken.

[0042] Liquid chromatography conditions:

[0043] High-performance liquid chromatography equipped with a fluorescence detector (Agilent 1260, Agilent, USA);

[0044] Chromatographic column: Phenomenex Kinetex C18, 100 × 4.6mm (inner diameter), 2.6 μm (particle size); ...

Embodiment 2

[0052] Example 2 Detection of rhododendron in cosmetics

[0053] (1) Sample handling

[0054] Weigh 0.20 g of the sample to be tested (accurate to 0.001 g), put it in a 10 mL plastic centrifuge tube, add 0.15 mL of 0.02 mol / L ammonium acetate aqueous solution, vortex for 30 s, and add 5% trichloroacetic acid-acetonitrile to mix Extraction (70:30, V / V) 1.85 mL, vortexed for 60 s, ultrasonically extracted for 15 min, then centrifuged at 10,000 rpm for 10 min, the supernatant was passed through a 0.22 μm microporous membrane to obtain the extract solution, Standby; the above-mentioned samples to be tested are respectively cream, lotion, water and facial mask.

[0055] (2) Liquid chromatography conditions

[0056] High-performance liquid chromatography equipped with a fluorescence detector (Agilent 1260, Agilent, USA);

[0057] Chromatographic column: Phenomenex Kinetex C18, 100 × 4.6mm (inner diameter), 2.6 μm (particle size);

[0058] Mobile phase: A: formic acid aqueous sol...

Embodiment 3

[0067] Example 3 Detection of rhododendol in cosmetics with a concentration of 1 mg / kg rhododendron

[0068] (1) Sample handling

[0069] Using the same cream, lotion, lotion and facial mask as the base in Example 2, prepare a test sample of cream, lotion, lotion and facial mask containing rhododendol at a concentration of 1 mg / kg.

[0070] Weigh 0.20 g of the sample to be tested (accurate to 0.001 g), put it in a 10 mL plastic centrifuge tube, add 0.15 mL of 0.02 mol / L ammonium acetate aqueous solution, vortex for 30 s, and add 5% trichloroacetic acid-acetonitrile to mix Extraction (70:30, V / V) 1.85 mL, vortexed for 60 s, ultrasonically extracted for 15 min, then centrifuged at 10,000 rpm for 10 min, the supernatant was passed through a 0.22 μm microporous membrane to obtain the extract solution, spare.

[0071] (2) Liquid chromatography conditions

[0072] High-performance liquid chromatography equipped with a fluorescence detector (Agilent 1260, Agilent, USA);

[0073] ...

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Abstract

The invention discloses a method for detecting phadodendrol in whitening cosmetics through high performance liquid chromatography. The method comprises the following steps: adding ammonium acetate aqueous solution into a to-be-detected sample, then adding 5% trichloroacetic acid-acetonitrile solution and then performing ultrasonic extraction, centrifuging extraction solution, filtering supernatant through a microfiltration membrane and then performing high performance liquid chromatography determination. A fluorescent detection is adopted for detection, the fluorescent excitation and emission wavelengths are respectively 280nm and 315nm. The method for determining the phadodendrol through high performance liquid chromatography is fast, simple and convenient in pretreating, low in detection limit, and high in recovery rate.

Description

technical field [0001] The invention belongs to the field of cosmetic analysis, in particular to a method for detecting rhododendron in cosmetics. Background technique [0002] The chemical name of rhododendron is 4-(4-hydroxyphenyl)-2-butanol. The pure product is white powder, soluble in water and organic solvents such as methanol, ethanol and acetonitrile. Rhododendrol is mainly found in medicinal plants such as birch, aceraceae, fir and turmeric, and is mainly used as a raw material for the biocatalytic synthesis of spices (such as raspberry ketone and betulin); due to its structure and tyrosinase activity inhibitors Similar to most of them having a phenolic structure, rhododendol has the effect of inhibiting the synthesis of melanin, so it is also used as a whitening ingredient in cosmetics. However, it is precisely because of the effect of inhibiting the synthesis of melanin that it interferes with the normal enzymatic conversion process of tyrosine into melanin in the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 徐振东石建华潘葵顾娟红余雯静郑利陈军朱振华
Owner 苏州出入境检验检疫局检验检疫综合技术中心
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