Polyethylene glycol-polysarcosine double hydrophilic block copolymer and its synthesis method
A block copolymer, polyethylene glycol technology, applied in the synthesis field of polyethylene glycol-polysarcosine double hydrophilic block copolymer
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Embodiment 1
[0042] Add 0.395g (3.4mmol) of Sar-NCA to the reaction flask, dissolve it with 1.5mL N,N-dimethylformamide, then add 0.31mL mPEG200-NH 2 DMF solution (0.022mol / L, 0.0068mmol), Sar-NCA and mPEG200-NH 2 The molar ratio is 500:1, sealed and placed in a 40°C oil bath to react for 24 hours. After the reaction, the mixture is poured into diethyl ether to precipitate, filtered, and the obtained polymer is vacuum-dried to obtain a polypeptide with a yield of 95 %. The NMR number average molecular weight of the obtained polymer was 33.9 KDa, and the molecular weight distribution was 1.2.
Embodiment 2
[0044] Other polymerization conditions are identical with embodiment 1, difference is to use mPEG5000-NH 2 As the initiator to initiate the ring-opening polymerization of Sar-NCA, the solvent is N,N-dimethylacetamide, Sar-NCA and mPEG5000-NH 2 The molar ratio was 5:1, and the reaction was carried out in a constant temperature bath at 0°C for 4 days, and the yield of the obtained peptide was 90%. The NMR number average molecular weight of the obtained polymer was 5.4KDa, and the molecular weight distribution was 1.1.
Embodiment 3
[0046] Other polymerization conditions are identical with embodiment 1, difference is to use NH 2 -PEG10000-NH 2 As the initiator to initiate the ring-opening polymerization of Sar-NCA, the solvent is N-methylpyrrolidone, Sar-NCA and NH 2 -PEG10000-NH 2 The molar ratio was 100:1, and the reaction was carried out in a constant temperature bath at 80°C for 12 hours, and the yield of the obtained polypeptide was 100%. The NMR number-average molecular weight of the obtained polymer was 17.1 KDa, and the molecular weight distribution was 1.2.
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