A method for immobilizing electrochemiluminescence on the surface of an electrode

Abstract

The invention discloses a method for fixing electrochemical luminophor on the surface of an electrode. The method is characterized by comprising the following specific steps: adding an electrochemical luminophor solution and an amino acid solution into a buffer solution, uniformly mixing up to obtain a mixture solution, introducing nitrogen to remove oxygen for 5-8 minutes, arranging the electrode into the mixture solution, applying potential being +1.8 to +2.2V onto the electrode for 10-240 seconds so as to accomplish the operation of stably fixing the electrochemical luminophor on the surface of the electrode, wherein the structural formula of the electrochemical luminophor is MP3X2, wherein M is Ru, P is a ligand, at least one of three ligands is a derivative with amino, and X is a monovalent anion. The method has the advantages that the electrochemical luminance is good, the stability is excellent, the operation is simple, the time is short, simple equipment is needed, the cost is low, and later development is easy to achieve.

Description

technical field [0001] The invention relates to the field of electrochemiluminescence detection, in particular to a method for fixing an electrochemiluminescence body on an electrode surface. Background technique [0002] Electrochemiluminescence (ECL) refers to the use of electrodes to apply a certain voltage or current to a system containing an electrochemiluminescent substance, so that the electrochemiluminescent substance transitions to an excited state by participating in an electrochemical reaction to obtain energy, and then returns to the ground state. luminescence phenomenon. As an analytical technique that combines electrochemistry and chemiluminescence, electrochemiluminescence not only has the advantages of high sensitivity, wide linear range, convenient observation, and simple instrument of chemiluminescence analysis, but also has the potential controllability of electrochemical analysis. With the advantages of strong reactivity, high selectivity, stable reagent...

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Problems solved by technology

[0004] At present, the methods used to directly immobilize ECL on the electrode surface include: Langmuir-Blodgett membrane method, molecular self-assembled membrane method, Nafion membrane method, sol-gel method, electrode surface sulfhydryl functionalization combined with ECL-Au nanometer Particle aggregate method, polymer / carbon nanotube composite membrane method, graphene adsorption method, etc., but these methods are generally cumbersome to operate, and each has obvious shortcomings: Langmuir-Blodgett membrane method is physical adsorption, easy to desorb, The stability is poor, and the equipment used is expensive; the molecular self-assembled membrane method, the electrode surface thiol functionalization combined with the electrochemiluminescent body-Au nanoparticle aggregate method uses a certain reducing sulfhydryl group, and the electrochemical response is irreversible and unstable. easy desorption; Nafion membrane method and polymer / carbon nanotube composite membrane method have relatively dense membranes, poor conductivity, and difficult electron transfer. Even if carbon nanotubes are added, the improvement is limited and the stability is not good; sol-gel The electrochemiluminescent body is fixed in the gel film by the method, but the pore size of the gel hole is usually larger than the small volume of the electrochemiluminescent body, so the leakage phenomenon is inevitable; the graphene adsorption method uses graphene to adsorb the electrochemiluminescence through the π-π effect Body, weak force, difficult to obtain stable results

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