Method for extraction and content determination of loganin

A method of determination, the technology of loganin, applied in the field of determination, can solve the problems of inaccurate determination and complicated operation, and achieve the effect of simple operation and accurate determination

Inactive Publication Date: 2014-05-21
陶建臣
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Problems solved by technology

Existing methods for extraction and determination of loganin conten...

Method used

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Embodiment Construction

[0014] The present invention adopts the following technical solutions.

[0015] (1) Take loganin and add methanol to make a solution containing 60 μg loganin per 1 mL.

[0016] (2) Take the powder of the product to be tested, pass it through a No. 3 sieve, put it in a stoppered Erlenmeyer flask, add 80% methanol, after ultrasonic treatment for 30 minutes, centrifuge for 20 minutes, and take the supernatant and filter it through a microporous membrane.

[0017] (3) Inject loganin reference substance solution for 3 to 10 times, and the peak area RSD is 0.84%.

[0018] (4) Weigh the medicinal material powder and calculate the loganin content, and the RSD is 1.07%.

[0019] (5) Weigh 6 parts of medicinal material powder with known content, put them in a round bottom flask, add loganin reference substance solution, and carry out determination.

[0020] The power of the ultrasonic treatment in the step (2) is 250W, and the frequency is 50kHz.

[0021] In the step ...

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Abstract

The invention especially relates to a method for extraction and content determination of loganin, belonging to the technical field of determination methods. The method provided by the invention has the advantages of simple operation and accurate determination. According to a technical scheme in the invention, the method comprises the following steps: weighing loganin and adding methanol to prepare a loganin solution with a concentration of 60 mu g/ml; weighing to-be-determined powder, sieving the powder with a sieve No. 3, placing the sieved powder into a cone flask with a plug, adding 80% methanol, carrying out ultrasonic treatment for 30 min, then carrying out centrifugation for 20 min, taking a supernatant and subjecting the supernatant to microporous membrane filtration; taking a loganin reference solution and carrying out sample introduction 3 to 10 times, wherein a peak area RSD is 0.84%; weighing medicinal powder and calculating loganin content, wherein RSD of the medicinal powder is 1.07%; and weighing 6 parts of the medicinal powder with known content, putting the medicinal powder into a round bottom flask, adding the loganin reference solution and carrying out determination.

Description

Technical field [0001] The present invention is a technical field of measurement method, especially involving a partaride content extraction and measurement method. Background technique [0002] The winged grass is also known as the poisonous bangs. It is the dry whole grass of the spoon of the plant spoon of the Sichuan family. It is a large Tibetan medicinal material.The winged grass has the effects of detoxifying and eliminating plague, clearing heat and stopping diarrhea, and removing wind and paralysis; treating external sensation, rheumatism and hypothyroidism, which are clinically used to treat rheumatoid arthritis.At present, the quality of the first herbal medicinal materials is controlled by determining the free -sulcoside oligosidoside oligosic acid and cyclidic acid. It has not yet seen reports of the content of other compounds in the content of the wing -headed herbal medicinal materials or its preparations.Marcin is an important chemical composition in the winged gr...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 陶建臣陶呈君
Owner 陶建臣
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