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Preparation technology of tremella sporophore polysaccharide

A technology of Tremella seeds and entities, applied in the field of Tremella polysaccharide and its extraction and preparation, can solve the problems of chemical solvent residue, chemical reagent residue extraction time, low extraction efficiency and the like

Active Publication Date: 2014-05-28
NANJING INST FOR THE COMPREHENSIVE UTILIZATION OF WILD PLANTS CHINA COOP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Through comparison, it is also found that the traditional method has low extraction efficiency, high energy consumption, low polysaccharide purity, and the use of organic solvents such as chloroform, isoamyl alcohol or chemical inorganic salts (such as sodium hydroxide, hydrochloric acid) in the extraction and purification process, There are defects such as chemical solvent residues and unstable product quality, and the application field is narrow
[0004] The purpose of the present invention is to overcome the disadvantages of using toxic and harmful chemical reagents in the existing tremella polysaccharide extraction process, the product has residual chemical reagents, long extraction time, and large energy consumption

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0015] step 1 Select 1 kg of commercially available white fungus with no impurities and cut off the darker part of the base of the fruit body, put it in a countercurrent extractor, add 5 kg of 70% ethanol, and extract it at 60 ° C for 2 hours, then filter it with a filter cloth Remove residual ethanol, soak 3 hours with 12 kilograms of clear water; Extrude and break the wall of the white fungus fruiting body that imbibes with differential speed double-roll extruder, the gap between the double-roll extrusion is 0.05 mm, and the double-roll extrusion mainly The roll speed is 300 rpm, the auxiliary roll speed is 100 rpm, and the extrusion is 3 times.

[0016] step 2 Add 20 kg of water to the extruded fruiting bodies, and add 10 grams of trypsin and 1.5 grams of pectinase to the slurry, and incubate at 53°C for 4 hours for enzymolysis. After the enzymolysis, filter with a 400-mesh filter cloth to remove Enzymolysis solution, and washed 3 times with warm water at 50°C to remove...

Embodiment example 2

[0019] step 1 Weigh 2 kg of commercially available white fungus fragments, remove variegated fragments and impurities, place them in a countercurrent extractor, add 8 kg of 95% ethanol, extract at 50 ° C for 3 hours, filter with a filter cloth to remove residual ethanol, and use Soak in 20 kilograms of clear water for 2 hours; the white fungus fruiting bodies that absorb the swelling are extruded and broken by a differential speed double-roll extruder. / min, the secondary roll speed is 200 rpm, and the extrusion is 3 times.

[0020] step 2 Add 50 kg of water to the extruded fruiting bodies, and add 20 grams of trypsin and 3 grams of pectinase to the slurry, and incubate at 50°C for 4 hours for enzymolysis. After the enzymolysis, filter with a 400-mesh filter cloth. Remove the enzymatic solution, and wash it with warm water at 40°C to remove residual biological enzymes and enzymatic products, which are the raw materials for polysaccharide extraction.

[0021] step 3 A...

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Abstract

The invention relates to a preparation technology of tremella sporophore polysaccharide, which is a tremella polysaccharide extraction technology combining a counter flow decoloration, roller extrusion grinding and biological enzyme deproteinization process and a high-pressure extraction and ceramic membrane separation process. The preparation technology is characterized by comprising the following steps: after performing counter flow decoloration on tremella sporophore by 70 percent of ethanol, carrying out roller extrusion grinding and carrying out composite biological enzymolysis deguming deproteinization, carrying out extraction for 2 to 4 hours with 50 to 80 times of water at a temperature of 105 to 121 DEG C; carrying out impurity removal on extracting solution by a 0.5 to 5.0micrometer ceramic membrane and concentrating the extracting solution by a 0.01micrometer ceramic membrane; carrying out ethanol precipitation and vacuum drying to obtain the tremella polysaccharide. When the technology is applied to prepare the tremella sporophore polysaccharide, the extraction rate can reach over 90 percent; the prepared polysaccharide is a mixture of acid polysaccharide and neutral polysaccharide; the polysaccharide content is 85 percent to 92 percent; the ash content is 5.4 percent to 6.73 percent; the protein content is 1.3 percent to 1.5 percent; the tremella sporophore polysaccharide has excellent emulsifying and moisturizing properties and can be applied to the fields of foods, drugs and daily chemical.

Description

technical field [0001] The invention belongs to the technical field of bioactive component extraction, and in particular relates to tremella polysaccharide and its extraction and preparation. Background technique [0002] Tremella polysaccharide is an active polysaccharide extracted from Tremella fruiting bodies or spores. Modern structural analysis proves that tremella polysaccharide is a kind of heteropolysaccharide, including acid heteropolysaccharide, neutral heteropolysaccharide, cell wall polysaccharide, exopolysaccharide and acid oligosaccharide and other components. A large number of studies at home and abroad have proved that tremella polysaccharides have excellent physiological effects such as improving human immune function, anti-virus, anti-tumor, anti-ulcer, lowering blood fat, blood sugar, and cholesterol, as well as physical and chemical functions such as moisturizing, thickening, and emulsifying. Food, medicine, daily chemicals and other fields have received...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 赵伯涛黄晓德钱骅朱羽尧陈斌
Owner NANJING INST FOR THE COMPREHENSIVE UTILIZATION OF WILD PLANTS CHINA COOP