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Application of met gene and kit for detecting gastrointestinal stromal tumor

A gastrointestinal stromal tumor and kit technology, applied in the field of medicine, can solve the problems of increasing the risk of intraperitoneal implantation and local recurrence, and achieve the effect of excellent use and adjuvant treatment

Active Publication Date: 2015-12-02
北京圣谷智汇医学检验所有限公司
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Problems solved by technology

Some patients see a doctor because of ulcer perforation, which can increase the risk of abdominal implants and local recurrence

Method used

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  • Application of met gene and kit for detecting gastrointestinal stromal tumor
  • Application of met gene and kit for detecting gastrointestinal stromal tumor
  • Application of met gene and kit for detecting gastrointestinal stromal tumor

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Experimental program
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Embodiment approach

[0060] According to a typical embodiment of the present invention, a method for detecting gastrointestinal stromal tumor is provided. The method includes the following steps: extracting genome DNA, sequencing the MET gene; comparing the MET gene sequence, and judging whether there is a gene mutation in the MET gene. If there is a gene mutation, it means that the patient may have gastrointestinal stromal tumor, which can be confirmed by other diagnostic methods in one step. Preferably, it is determined whether there is a gene mutation at positions A to G at positions 116340262 of chromosome 7 in the MET gene, namely N375S.

[0061] The sequencing in the present invention can be performed by conventional sequencing methods in the field. Preferably, the MET gene is sequenced by using IonTorrentPGM sequencer or PCR combined with sanger method. These two sequencing methods have high sequencing efficiency and accurate sequencing.

[0062] Among them, sequencing using the IonTorrent...

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Abstract

The present invention discloses an application of a MET gene, and a kit for detecting a gastrointestinal stromal tumor, wherein the kit comprises MET gene sequencing primers. According to the present invention, the inventor finds that: the gastrointestinal stromal tumor is caused by the common KIT and PDGFRA gene mutations, and the MET (N375S) mutation is one of the causes of the gastrointestinal stromal tumor, such that the kit for detecting the gastrointestinal stromal tumor can be provided for rapidly and accurately detecting the MET mutation site of the gastrointestinal stromal tumor and diagnosing the gastrointestinal stromal tumor so as to treat the gastrointestinal stromal tumor based on the finding; and after the MET (N375S) mutation is detected by using the kit, the gene targeting drug can be adopted to specifically treat patients, such that the use of the targeting drug is specific.

Description

technical field [0001] The invention relates to the field of medicine, in particular to an application of the MET gene and a kit for detecting gastrointestinal stromal tumors. Background technique [0002] Gastrointestinal stromal tumor (GIST) is the most common mesenchymal tumor of the digestive tract, and was often misdiagnosed as leiomyoma or leiomyosarcoma in the past. In 1983, Mazur and Clark first proposed the concept of GIST, and believed that its origin was related to the Cajal cells in the muscular layer of the gastrointestinal tract. In recent years, the understanding of this disease has gradually deepened. It is generally believed that GIST is a mesenchymal tumor expressing the growth factor receptor KIT protein with tyrosine kinase activity. [0003] Gastrointestinal stromal tumors account for 1-3% of gastrointestinal malignant tumors, and the estimated annual incidence is about 10-20 / 1 million. They mostly occur in middle-aged and elderly patients, and are rare...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6886C12Q2600/156
Inventor 叶桦陈锦飞楼峰唐川宁陈思毅
Owner 北京圣谷智汇医学检验所有限公司
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