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Anaerobic Bacillus ha and its application in degrading nitrogen oxides

A technology of anaerobic bacillus and culture, applied in the application field of biological treatment of nitrogen oxides, can solve the problems of low regeneration rate, high cost, and no longer have the ability to complex and absorb NO, and achieve a strong heat resistance Effect

Active Publication Date: 2016-06-15
ZHEJIANG UNIV OF TECH
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, the absorption product produced by the complexation denitrification process no longer has the corresponding complexation absorption NO capacity, so the denitrification solution needs to be regenerated before it can be recycled.
In order to realize the regeneration of the absorption liquid, it is possible to add SO 3 2- 、Na 2 S 2 o 4 and other reducing agents, but the cost is high and the regeneration rate is low. Moreover, the reduction process is only for Fe with the ability to complex and absorb NO formed due to the presence of oxygen in the flue gas. III (EDTA), for another absorption product Fe II (EDTA) - NO appears to have no good countermeasures

Method used

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  • Anaerobic Bacillus ha and its application in degrading nitrogen oxides
  • Anaerobic Bacillus ha and its application in degrading nitrogen oxides
  • Anaerobic Bacillus ha and its application in degrading nitrogen oxides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Separation, purification and identification of Anoxybacillus contaminans HA

[0031] 1. Separation and purification of Anoxybacillus contaminans HA

[0032] AnoxybacilluscontaminansHA is selected from the sludge of the BioDeNOx reactor of our research group. The specific steps are as follows:

[0033] Basic medium, the preparation method is as follows: glucose 2000mg·L -1 , KH 2 PO 4 500mg·L -1 , K 2 HPO 4 500mg·L -1 , MgSO 4 ·7H 2 O100mg·L -1 , CaCl 2 100mg·L -1 , CuSO 4 1mg·L -1 , FeSO 4 1mg·L -1 , MnSO 4 5mg·L -1 , Na 2 MoO 4 1mg·L -1 , ZnCl 2 2mg·L -1 , The solvent is water, pH 7.0, and sterilization at 121°C for 20 minutes.

[0034] Fe II (EDTA)-NO solution preparation method: use equimolar FeSO 4 ·(NH 4 ) 2 SO 4 ·6H 2 O and Na 2 EDTA is configured as Fe in oxygen-driven deionized water II (EDTA) solution, then use 1mmol / L NaOH aqueous solution to prepare Fe II (EDTA) Adjust the pH value of the solution to about 7.0, pour it into a 500mL absorption bottle, pass i...

Embodiment 2

[0048] Example 2 Anaerobic Bacillus HA fermentation broth

[0049] (1) Inclined surface cultivation

[0050] The anaerobic bacillus HA was inoculated into a slant medium, cultured at 55°C for 3 days to obtain a bacterial slant; the final concentration of the slant medium was composed of 2000 mg·L glucose -1 , NaNO 3 1000mg·L -1 , KH 2 PO 4 500mg·L -1 , K 2 HPO 4 500mg·L -1 , MgSO 4 ·7H 2 O100mg·L -1 , CaCl 2 100mg·L -1 , CuSO 4 1mg·L -1 , FeSO 4 1mg·L -1 , MnSO 4 5mg·L -1 , Na 2 MoO 4 1mg·L -1 , ZnCl 2 2mg·L -1 , The solvent is water, pH value is 7.0, agar 18g·L -1 .

[0051] (2) Seed cultivation

[0052] Pick a colony from the slant surface of the bacterial cell and inoculate it into a seed culture medium, culture at 55°C for 1 day to obtain a seed liquid; the final concentration of the seed culture medium is composed of 2000 mg·L glucose -1 , NaNO 3 200mg·L -1 , KH 2 PO 4 500mg·L -1 , K 2 HPO 4 500mg·L -1 , MgSO 4 ·7H 2 O100mg·L -1 , CaCl 2 100mg·L -1 , CuSO 4 1mg·L -1 , FeSO 4 1mg·L...

Embodiment 3

[0055] Example 3: Denitrification performance test of Anoxybacillus contaminans HA

[0056] 1. Investigate the reduction of Fe by anaerobic bacillus HA under different initial pH conditions II (EDTA)-NO performance

[0057] The experiments of reducing Fe(II)EDTA-NO by anaerobic bacillus HA at different initial pHs have found that the pH has high denitrification ability at pH 7.0. From the perspective of practical application, pH=7.0 is the best pH. When the degradation rate is the highest, the specific implementation steps are as follows:

[0058] Take Fe II (EDTA)-NO is the only nitrogen source (concentration 5mmo1·L -1 ), according to the volume concentration of 1% inoculum 600 0.4 bacteria-containing suspension (prepared by the method in Example 2) was inoculated into Fe with different pH values II (EDTA)-NO liquid selective medium (pH value respectively 5.0, 6.0, 7.0, 8.0, 9.0), 55℃, 160rpm shaking culture 24h, obtain the culture solution, adopt spectrophotometric method to measu...

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Abstract

The invention discloses a new strain-Anoxybacillus contaminans HA and application thereof in degrading oxynitrides. The Anoxybacillus contaminans HA is collected at China Center for Type Culture Collection, of which the address is Wuhan University, Wuhan, China and the postal code is 430072; and the collection number is CCTCC NO:M2013678, and the collection date is December 20th, 2013. The invention provides an Anoxybacillus contaminans HA with high efficiency, high heat resistance and high FeII(EDTA)-NO reducibility and application thereof in degrading oxynitrides. The reduction rate of the strain for FeII(EDTA)-NO with the initial concentration of 5 mmol.L<-1> within 1 day is up to 95.3%; and the detection of the degradation bacterium has important meanings for high-efficiency purification of oxynitrides in industrial waste gas.

Description

(1) Technical field [0001] The present invention relates to a strain with Fe II (EDTA)-NO-reducing anaerobic Bacillus HA, and its application in biological treatment of nitrogen oxides. (2) Background technology [0002] Nitrogen oxides (NOx) are one of the main pollutants emitted from the combustion of fossil fuels, of which more than 95% is NO, and the rest is mainly NO 2 . Large amounts of nitrogen oxides with local, regional, and global pollutant properties can cause various environmental problems, such as photochemical smog, acid rain, and ozone layer destruction. At the same time, nitrogen oxides also pose a great threat to human health and are an important component of fine particulate matter (PM2.5); and many research teams have confirmed that NO is directly related to neurodegenerative diseases such as Parkinson's disease. [0003] With the rapid development of my country's national economy, the demand for energy, especially fossil fuels, has become increasingly strong, w...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B01D53/84B01D53/56C02F3/34C12R1/01C02F101/16
CPCY02A50/20
Inventor 陈浚郝红红张慧慧王磊李彦程鳌陈建孟
Owner ZHEJIANG UNIV OF TECH
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