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A device and method for using plasma to act on biological materials
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A plasma and generating device technology, applied in the field of biological material processing devices, can solve problems affecting the normal use of plasma devices, contamination of biological samples, inability to ensure discharge space and cleanliness of the processing process, etc.
Active Publication Date: 2017-01-11
WUXI TMAXTREE BIOTECHNOLOGY CO LTD
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Problems solved by technology
At present, many researchers use different types of plasma to treat different microorganisms and obtain good mutant strains. However, these experimental devices either process biological samples in an open environment, or although they are relatively closed spaces, However, the closed space cannot be sterilized or effectively isolated from the external environment during operation, which may easily lead to contamination of biological samples and seriously affect the normal use of plasma devices in research and production.
When Yu Hong et al. used DBD plasma for mutation breeding of microorganisms, they used the method of manual wiping to disinfect the inner surface of the DBD plasma device, but it could not guarantee the cleanliness of the entire discharge space and the treatment process.
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experiment example 1
[0123] Experimental example 1. The operation process of using the device of the first group of embodiments of the present invention to process biological materials
[0124] Experimental material: dealing with microbial suspension in logarithmic phase of growth, without pretreatment
[0125] Steps:
[0126] (1) Open the sample hatch
[0127] (2) Place the material to be processed in the sample area
[0128] (3) Close the sample hatch
[0129] (4) Turn on the disinfection device to disinfect the entire clean working cabin. After the disinfection is completed, open the ventilation device to empty the chemical residues in the clean working cabin to ensure that the cleanliness of the working cabin reaches more than 100,000 grades.
[0130] (5) In the case of ensuring that the sample area and the processing area are clean, the sample is moved manually or using an automatic device and exposed to the plasma jet area for processing. The air pressure in the plasma action area is cont...
experiment example 2
[0134] Experimental example 2. The operation process of using the device of the first group of embodiments of the present invention to process biological materials
[0135] Experimental material: dealing with microbial suspension in logarithmic phase of growth, without pretreatment
[0136] The specific operation steps are as follows:
[0137] (1) Open the sample hatch
[0138] (2) Place the materials to be processed on the loading bracket of the working cabin,
[0139] (3) Close the sample hatch
[0140] (4) Turn on the disinfection device to disinfect the entire clean working cabin. After the disinfection is completed, open the ventilation device to empty other residual substances in the clean working cabin, so that the cleanliness level in the working cabin can reach more than 100,000.
[0141] (5) Adjust the loading bracket to move the sample into the plasma discharge area, start the plasma generating device for processing, the air pressure in the plasma action area is ...
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Abstract
The present invention, "a device and a method for acting on biomaterials by using plasma" relates to a biomedical device and is characterized in that: the device comprises a plasma generating device (1) and a clean working bin (2) with no pollutants having biological activity; a sample area (3) is provided within the clean working bin (2) and the working area for plasma generation by the plasma generating device (1) is within the clean working bin (2), wherein the sample area (3) is within the working area; and at least one site for installing a disinfection device is reserved in the cavity or on the wall of the clean working bin. It can be ensured that plasma efficiently and quickly processes the biomaterial without being contaminated by other impurities, microbes, etc., thus having a good application value in the biological field.
Description
technical field [0001] The invention relates to a processing device for biological materials, in particular to a device and method for using plasma to act on biological materials. Background technique [0002] Plasma is the fourth state of matter. It is a material form mainly composed of a large number of free electrons and charged ions. It is electrically neutral macroscopically. Charged particles (electrons or ions) in a plasma are usually energy-rich. Studies have shown that a large number of energetically active particles in plasma can act on biological samples and cause changes in various target traits, showing their good application prospects in the field of biotechnology. [0003] There are still very few applications of plasma in the biological field. Li Guo et al. used exposed electrodedischarge to generate atmospheric pressure and room temperature plasma to treat artificially constructed plasmidDNA in an open environment, which can cause DNA fragmentation. At ...
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