Apparatus and method for extracting biologically active substances
A technology for biologically active substances and instruments, applied in the field of automated instruments for extracting biologically active substances, can solve problems such as affecting elution efficiency and purity
Inactive Publication Date: 2015-09-16
GENMAG BIOTECH
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- Abstract
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Problems solved by technology
As the suction method does not remove the magnetic beads when removing the waste liquid, the pipette should not be too close to the magnetic beads to prevent the magnetic beads together with the waste liquid from being sucked out, affecting the elution efficiency and purity
Method used
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Embodiment Construction
[0091] In order to better understand and illustrate the present invention, the present invention will be further described in detail below with reference to the accompanying drawings.
[0092] figure 1 is a first-direction perspective view of the interior of the apparatus for extracting bioactive substances according to a preferred embodiment of the present invention. The apparatus of the present invention can be used to extract and purify nucleic acids. The instrument has a housing (not shown in the drawings) and a base plate 1, a magnetic needle 7 and a magnetic needle moving mechanism 48, a sleeve 4 and a sleeve moving mechanism 16, a bracket 13 for supporting the magnetic needle moving mechanism and the sleeve moving mechanism , and a vibrating mechanism 19 for vibrating so that the casing moving mechanism vibrates up and down.
[0093] An enclosure (not shown in the figure) isolates the interior of the instrument from the atmosphere to prevent sample contamination and d...
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The invention provides an efficient automated instrument for extraction of bioactive substances. The instrument comprises: a housing, a base plate, a workbench, magnetic needles and a magnetic needle moving mechanism, casing pipes and a casing pipe moving mechanism, and a vibrating mechanism. The vibrating mechanism can generate vibration to make the casing pipe moving mechanism vibrate up and down. Specifically, the magnetic needles can be vertically inserted into and removed from the casing pipes, and the casing pipes can be vertically inserted into and removed from a sample treatment area. The vibrating mechanism comprises a lever arm, a movable fulcrum and a rotating mechanism. According to the arrangement, no matter how the vibration amplitude changes, the lowest point of the amplitude does not change. The invention also provides a method using the instrument to extract and purify bioactive substances, particularly nucleic acid. The instrument provided by the invention can be arbitrarily adjust the vibration frequency and amplitude, greatly improve the adjustment range of vibration frequency, and has the advantages of low noise and long life.
Description
technical field [0001] The invention relates to the fields of biology and instrumentation. Specifically, the present invention provides an automatic instrument for extracting biologically active substances, and a method for extracting biologically active substances using the instrument. Background technique [0002] The extraction of biologically active substances, such as cells, and biologically active substances such as proteins and nucleic acids, plays an important role in modern clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbial detection, food safety detection, molecular biology research and other fields. Among them, nucleic acid is a biologically active substance compound synthesized by many nucleotides, which is one of the most basic substances of life. Nucleic acid is widely present in all animals, plant cells, microorganisms, and organisms. According to different chemical composition, nucleic acid can be divided ...
Claims
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IPC IPC(8): C12M1/42C12M1/36C12N15/10C12N13/00C12N5/00C07K1/14
CPCC07K1/145C12M47/04C12N15/1013
Inventor 刘新志赵海峰
Owner GENMAG BIOTECH