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Separation, extraction and verification method of air-dried sludge beneficial microorganism components

A technology of air-dried sludge and identification method, which is applied in the direction of microorganism-based method, measurement/inspection of microorganism, biochemical equipment and method, etc., and can solve the problems that there are no literature reports on the utilization of microorganisms in urban sludge, and there are few classification and identification.

Inactive Publication Date: 2014-07-23
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still few studies on the classification and identification of beneficial microorganisms in municipal sludge, and there is no literature report on the utilization of municipal sludge microorganisms.

Method used

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  • Separation, extraction and verification method of air-dried sludge beneficial microorganism components
  • Separation, extraction and verification method of air-dried sludge beneficial microorganism components
  • Separation, extraction and verification method of air-dried sludge beneficial microorganism components

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Experimental program
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Embodiment 1

[0102] A method for separating, extracting and identifying beneficial microbial components of air-dried sludge, characterized in that it is carried out according to the following steps:

[0103] (1) Selection of materials: municipal sludge comes from sewage treatment plants;

[0104] (2) Cultivation of strains: Weigh 10 g of sludge, put it into a conical flask filled with 90 g of sterile water, add glass beads, shake it on a shaker for 20 min, and prepare 10 g of sludge by dilution separation method. －2 , 10 －3 、10 －4 、10 －5 、10 －6 sludge suspension; According to the isolated microorganisms, take different concentrations of sludge suspension, shake well, draw 0.2 mL and add it to the pre-poured plate;

[0105] 1) Use Martin's agar plate for fungi, add 3.3 ml of 1% Bengal red aqueous solution to 1000 ml of this culture medium, add 0.3 ml of 1% streptomycin solution to every 100 ml of medium before use; use Gaoshi No. 1 agar for actinomycetes Plate, add 2 drops of 10% pheno...

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Abstract

The invention discloses a separation, extraction and verification method of air-dried sludge beneficial microorganism components. Three strains, including one bacterium, one filamentous fungus and one actinomycete, are selected from 15 dominant strains, cultured and applied to a lawn plant construction system. According to the analysis on the actions of the 15 strains, pseudomonad, Trichoderma and streptomycete are finally selected. The pseudomonad, Trichoderma and streptomycete can generate active substances or improve mineral nutrition to directly promote the growth of plants, and can also inhibit or prevent the development of the rhizosphere pathogenic microorganisms by generating metabolites or through competition so as to indirectly promote the growth of plants.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and relates to methods for separating, extracting and identifying beneficial microbial components of air-dried sludge. Background technique [0002] Composite microbial agent is a kind of microbial preparation that is co-cultivated by two or more than two kinds of microbial strains that are not antagonistic to each other in an appropriate proportion, giving full play to the joint action advantages of the groups, and achieving better application effects. This type of microbial agent generally has It has the advantages of complete types, reasonable compatibility, quick effect, less investment, simple operation, and no secondary pollution. It has been widely used in various fields such as agriculture, industry, medicine, and environmental protection. [0003] Fertilization is one of the most important measures in agricultural production and lawn maintenance and management. At p...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C12Q1/68C12Q1/04C12R1/06C12R1/01C12R1/38C12R1/785C12R1/885C12R1/67C12R1/645C12R1/77C12R1/465C12R1/47
Inventor 多立安赵树兰王志旭
Owner TIANJIN NORMAL UNIVERSITY