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A method for extracting and recovering papain from waste residue after extracting papain

A technology of papain and papaya residue, which is applied in the field of biological protease extraction, can solve the problems of difficult extraction cost, high papain, and discarding of papaya residue, and achieve the effects of easy operation, high extraction recovery rate, and prevention of secondary pollution

Inactive Publication Date: 2016-08-17
深圳市湖大谷奥生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after papain is extracted from the milk of immature papaya fruit, a considerable amount of papaya residues will be produced, which still contains a certain amount of papain that is difficult to extract again or has a high extraction cost. At present, there is still no good method to synthesize it. Recycling, throwing away these papaya residues will bring certain impact to the environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Add 100Kg of waste papaya residue after extracting papain, add 5Kg of pure water, stir to form a paste, add 60g of cellulase, 20g of sodium metabisulfite, stir for 40 minutes, put the pasty papaya residue into a refiner for grinding, Then add 110Kg of water in the beater to slurry and mix, beat and stir for 30 minutes to make it into a suspension, filter with a 40-mesh filter cloth, there is basically no filter residue, add 110g of cysteine, 2Kg of cyclodextrin, and maltodextrin to the filtrate 2Kg, stir and mix evenly, and then spray dry. The process parameters are set as follows: the inlet air temperature is 150°C, the outlet air temperature is 70°C, and the feed frequency is 12Hz.

Embodiment 2

[0026] Add 200Kg of waste papaya residue after extracting papain, add 12Kg of pure water, stir to form a paste, add 100g of cellulase, 50g of ligninase, 20g of sodium metabisulfite and 25g of sodium benzoate, stir for 30 minutes, and make the paste of papaya residue Put it into a refiner for refining, then add 250Kg of water to the beater for pulping and mixing, beat and stir for 30 minutes to make it into a suspension, filter with a 60-mesh filter cloth, there is almost no filter residue, and add 231g of cysteine ​​to the filtrate , EDTA 231g, cyclodextrin 4Kg, stir and mix evenly and then spray dry. The process parameters are set as follows: the inlet air temperature is 160°C, the outlet air temperature is 75°C, and the feed frequency is 11Hz.

Embodiment 3

[0028] 300Kg of waste papaya residue after extracting papain, add 30Kg of pure water, stir to form a paste, add 165g of cellulase, 40g of sodium metabisulfite, stir for 50 minutes, put the pasty papaya residue into a refiner for grinding, Then add 400Kg of water in the beater for pulping and mixing, beating and stirring for 20 minutes to make it into a suspension, filter with 80 mesh filter cloth, a small amount of filter residue, add 380g of ascorbic acid, 7Kg of cyclodextrin, and 5Kg of maltodextrin to the filtrate, stir and mix After uniformity, spray drying is carried out, and the process parameters are set as follows: the inlet air temperature is 170°C, the outlet air temperature is 65°C, and the feeding frequency is 12.5Hz.

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Abstract

The invention relates to a method for extracting and recovering papain from waste residue extracted from papai. The extraction method comprises the following steps: 1)adding an extraction solvent and an antiseptic; 2)performing pulp refining; 3)beating and stirring; 4)filtering; and 5)adding an enzyme activity protective agent and performing spray drying. The method has the advantages of simple and practical usage, the papain recovery rate through extraction is high, the method is simple and easy to operate, the zero discharge can be basically realized, secondary pollution is prevented, and the environment health is greatly protected.

Description

technical field [0001] The invention relates to the technical field of biological protease extraction, in particular to a method for comprehensively extracting and recovering papain from waste papaya residue after extracting papain. Background technique [0002] Papain (Papain) referred to as papain, also known as papaya enzyme, is a pure natural biological enzyme product extracted from the milk of immature papaya fruit and refined by modern bioengineering technology. It is a sulfhydryl group (-SH)-containing endopeptidase, which has protease and esterase activity, has a wide range of specificity, and has strong hydrolysis ability for animal and plant proteins, polypeptides, esters, amides, etc. At the same time, it also has a synthetic function, which can synthesize protein hydrolyzate into proteinoids. It is used in a wide range of industries, including: pharmaceutical industry, food industry, beauty cosmetics industry, feed industry, leather industry, textile industry an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/50
CPCC12N9/641C12Y304/22002
Inventor 莫荣
Owner 深圳市湖大谷奥生物科技有限公司
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