Atractylis lancea tissue culturing and rapid propagating method

A technology of tissue culture rapid propagation and herb atractylodes, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of easy degradation of quality, poor seed vitality, low seed reproduction coefficient, etc., and achieve the effect of alleviating the contradiction between supply and demand

Active Publication Date: 2014-08-06
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Artificial propagation of Atractylodes atractylodis can be propagated by both seeds and rhizomes, but the propagation coefficient of seed prop

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0024] Embodiment 1: A method of tissue culture and rapid propagation of Atractylodes lanceolata includes the following steps:

[0025] 1. Seed treatment and disinfection

[0026] Put a suitable size filter paper in the Petri dish, and select the wild Atractylodes lanceolata ( Atractylodes lancea (Thunb.)DC.) Seeds, after washing with detergent, add appropriate amount of water to soak (it’s better if the seeds are just out of reach) for 0.5h; after soaking, rinse repeatedly with running water, then rinse twice with distilled water; The seeds were soaked in 70% ethanol by volume for 30s, and then soaked in 0.1% mercuric chloride solution by mass for 15 minutes, rinsed with sterile water 3 times, and dried on sterile filter paper for later use.

[0027] 2. Establishment of aseptic culture system

[0028] The above-mentioned sterilized seeds are connected to a germination medium, one per bottle; they germinate after 14 days, and one month later, they grow into sterile seedlings with two...

Example Embodiment

[0041] Embodiment 2: It is basically the same as embodiment 1, except that:

[0042] The setting culture conditions involved in steps 2 to 6 are as follows: light intensity: 2500lx; light time: 15h / d; culture temperature: 24°C.

[0043] The disinfection and sterilization method in step (1) is: add clean water to soak the cleaned seeds for 1 hour, after soaking, rinse repeatedly with running water, rinse twice with distilled water, and then soak in 70% ethanol solution by volume. After 20 seconds, soak in 0.1% mercuric chloride solution for 18 minutes, and finally rinse with sterile water for 5 times, and absorb the water on sterile filter paper for later use.

[0044] In step (2), germinate under the set culture conditions for 10 days, and the pH of the germination medium is 6.2.

[0045] In step (3) the callus induction and differentiation medium is: MS + 0.5 mg / L 6-benzylamino adenine + 0.05 mg / L naphthalene acetic acid + 3% sucrose by mass + 0.75% by mass Agar, pH is 6.2.

[0046] ...

Example Embodiment

[0049] Embodiment 3: It is basically the same as embodiment 1, the difference is:

[0050] The setting culture conditions involved in step 2 to step 6 are as follows: light intensity: 2500lx; light time: 13h / d; culture temperature: 22°C.

[0051] The method of disinfection and sterilization in step (1) is: add clean water to soak the cleaned seeds for 0.7h, after soaking, rinse repeatedly with running water, rinse twice with distilled water, and then soak with 70% ethanol solution by volume For about 25s, soak in 0.1% mercuric chloride solution for 17 minutes, and finally rinse with sterile water 5 times, and absorb the water on sterile filter paper for later use.

[0052] In step (2), germinate under the set culture conditions for 13 days, and the pH of the germination medium is 6.0.

[0053] In step (3) the callus induction and differentiation medium is: MS + 1.5 mg / L 6-benzylamino adenine + 0.15 mg / L naphthalene acetic acid + 3% sucrose by mass + 0.75% by mass Agar, pH 6.0.

[0054...

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PUM

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Abstract

The invention discloses an atractylis lancea tissue culturing and rapid propagating method which comprises the following steps: seed processing, disinfecting and sterilizing; building a bacteria-free culturing system; callus inducting, splitting callosity into seedlings; sterile seedling propagating and culturing; sterile seedling root taking and culturing; seedlings acclimatizing and transplanting. According to the method, a large number of the sterile seedlings can be acquired in a short period through the tissue culturing and rapid propagating technology, the problem of atractylis lancea seedlings is solved, the efficient path for large-scale cultivation and production as well as seedling propagation is provided. Through the tissue culturing, the atractylis lancea seedlings with high quality are provided, on this basis, new species with high yield and high quality are bred, novel officinal resources are developed, the imbalance between supply and demand is alleviated and significance for protecting wild atractylis lancea is achieved.

Description

technical field [0001] The invention relates to a method for tissue culture and rapid propagation system, in particular to a method for tissue culture and rapid propagation of Atractylodes atractylodis. Background technique [0002] Cangzhu ( Atractylodes lancea (Thunb.) DC.) is a perennial herbaceous plant of the genus Atractylodes genus in the family Asteraceae, and its rhizome is used as medicine. Mao Atractylodes is pungent, bitter, and warm in nature, and returns to the spleen, stomach, and liver channels. Clinically, it is mainly used for the treatment of abdominal fullness, edema, beriberi, rheumatic arthralgia, wind-cold cold, night blindness and so on. In recent years, the comprehensive development of Atractylodes atractylodes has expanded the use of Atractylodes atractylodes. In addition to medicinal purposes, it can be used as an additive for various beverages and sachets for processing crafts. [0003] Atractylodes atractylodes is mainly distributed in hilly a...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 宋刚徐银曹正杨士虎宋金耀谢正林黄小忠韦颖
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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