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Method for converting straw to ethanol by auxiliary carbon dioxide enzymolysis and coupled fermentation in solvent

A carbon dioxide, assisted enzymatic hydrolysis technology, applied in the direction of fermentation, biofuel, etc., can solve the problems of cellulase inactivation, undeveloped solvent treatment, enzymatic hydrolysis and fermentation coupling, etc., to achieve the effect of recycling

Inactive Publication Date: 2014-08-06
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are some enzymes that can catalyze the reaction in the ionic liquid medium, the ionic liquid will inhibit the activity of cellulase, and the ions in the ionic liquid will destroy the hydrogen bond of the cellulase and inactivate the cellulase, so it has not been developed yet. Coupling system of solvent treatment, enzymatic hydrolysis and fermentation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] [EMIM] Coupling carbon dioxide-assisted enzymatic hydrolysis and fermentation to transform straw into ethanol in DEP

[0013] 1) Straw treatment by ionic liquid dissolution: Dissolve 30 g of corn stalks at 130°C with 300 g of ionic liquid 1-ethyl-3-methylimidazolium diethylphosphate ([EMIM]DEP), stir evenly with magnetic force, and keep warm for 20 min. Cool down to 50°C, bubble in CO 2 , CO 2 The molar final concentration reaches 12% of the ionic liquid [EMIM]DEP to obtain a solution.

[0014] 2) Carbon dioxide-assisted enzymatic hydrolysis: add 670g of water to the solution to make the final concentration of ionic liquid [EMIM]DEP 30% (w / w), then add cellulase 12500FPU, mix and stir, and keep warm at 50°C for enzymolysis. Obtain enzymatic solution.

[0015] 3) Microaerobic fermentation for ethanol production: after enzymolysis, adjust the pH to 4.5, inoculate Saccharomyces cerevisiae at 2% (v / v) in the enzymolysis solution, and stop passing CO 2 , sealed fermentat...

Embodiment 2

[0018] [EMIM] Coupling carbon dioxide-assisted enzymatic hydrolysis and fermentation to transform straw into ethanol in Ac

[0019] 1) Straw treatment by ionic liquid dissolution: 50 g of sugarcane straw was treated with 600 g of ionic liquid 1-ethyl-3-methylimidazolium acetate ([EMIM]Ac) at 120°C, stirred evenly by magnetic force, and kept for 20 min. Cool down to 40°C and bubble in CO 2 , CO 2 The molar final concentration reaches 18% of the ionic liquid [EMIM] Ac to obtain a solution.

[0020] 2) Carbon dioxide-assisted enzymatic hydrolysis: Add 5kg of water to the solution to make the final concentration of the ionic liquid [EMIM]Ac 10.62% (w / w), then add 70000FPU cellulase, mix and stir, and incubate at 50°C for enzymolysis.

[0021] 3) Ethanol production by microaerobic fermentation: After enzymolysis, adjust the pH to 5.0, add Candida shohata to the enzymolysis solution at 3% (v / v), stop CO 2 , Sealed fermentation for 24 hours to obtain alcohol, the yield can reach 6...

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PUM

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Abstract

The invention discloses a method for converting straw to ethanol by auxiliary carbon dioxide enzymolysis and coupled fermentation in a solvent. The method comprises the following steps: treating the straw with the solvent, introducing carbon dioxide, adding enzyme for dissolution, performing efficient in situ enzymolysis on the straw in a solvent system so as to degrade the straw into sugar, adjusting the pH value, and adding microbe for in situ fermentation so as to convert sugar into ethanol. After ethanol is extracted, salt is added to prepare a double water phase system, carbon dioxide can promote recycling of the solvent, and the straw solvent recycling rate can reach 99.5%. The method can convert the straw into bioethanol in an environment-friendly and efficient manner, the solvent, the enzyme and the microbe can almost totally be recycled for reutilization, no waste is generated in the whole system, the cost is low, and the method is suitable for large-scaled application.

Description

technical field [0001] The invention relates to a method for producing ethanol by coupling carbon dioxide-assisted enzymolysis and fermentation to convert straws into ethanol, in particular to a method for synchronously recovering and purifying an ionic liquid in a mixture solution. Background technique [0002] Since cellulose is insoluble in common solvents, its solvents and dissolution methods are very important topics in the cellulose industry and basic research. The solvent of cellulose can directly dissolve cellulose to achieve the purpose of pretreatment and retain the characteristics of natural cellulose to the greatest extent. It has long been hoped that cellulose can be directly dissolved in common solvents to become a transparent solution, and then processed into various products such as silk, film, filler, non-woven fabric, etc. Cellulose solvents are divided into non-derivatized solvents and derivatized solvents, and can also be divided into aqueous phase solve...

Claims

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Application Information

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IPC IPC(8): C12P7/10
CPCY02E50/10
Inventor 李斌李强冯圆圆季更生屠洁李天程赵翔
Owner JIANGSU UNIV OF SCI & TECH
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