Method for converting straw to ethanol by auxiliary carbon dioxide enzymolysis and coupled fermentation in solvent

A carbon dioxide, assisted enzymatic hydrolysis technology, applied in the direction of fermentation, biofuel, etc., can solve the problems of cellulase inactivation, undeveloped solvent treatment, enzymatic hydrolysis and fermentation coupling, etc., to achieve the effect of recycling

Inactive Publication Date: 2014-08-06
JIANGSU UNIV OF SCI & TECH
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are some enzymes that can catalyze the reaction in the ionic liquid medium, the ionic liquid will inhibit the activity of cellulase, and the ions in the ionic liquid wil

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0011] Example 1

[0012] [EMIM] Coupling of carbon dioxide assisted enzymatic hydrolysis and fermentation in DEP to convert straw to ethanol

[0013] 1) Ionic liquid dissolution treatment of straw: 300g of ionic liquid 1-ethyl-3-methylimidazole diethyl phosphate ([EMIM]DEP) is used to dissolve 30g of corn stalks at 130°C, magnetically stirred evenly, and kept for 20 minutes. Cool down to 50℃, bubbling into CO 2 , CO 2 The final molar concentration of the ionic liquid [EMIM] DEP is 12%, and the solution is obtained.

[0014] 2) Carbon dioxide-assisted enzymatic hydrolysis: Add 670g of water to the dissolution solution to make the final concentration of the ionic liquid [EMIM]DEP 30% (w / w), then add cellulase 12500FPU, mix well and stir, and keep the enzymatic hydrolysis at 50℃. Obtain the enzymatic hydrolysis solution.

[0015] 3) Microaerobic fermentation to produce ethanol: After enzymatic hydrolysis, adjust the pH to 4.5, add Saccharomyces cerevisiae in the enzymatic hydrolysis so...

Example Embodiment

[0017] Example 2

[0018] [EMIM] Coupling of carbon dioxide assisted enzymatic hydrolysis and fermentation in Ac to convert straw to ethanol

[0019] 1) Ionic liquid dissolving and treating straw: Use 600g of ionic liquid 1-ethyl-3-methylimidazole acetate ([EMIM]Ac) to treat 50g of sugarcane straw at 120°C, magnetically stir evenly, and keep it warm for 20min. Cool down to 40℃, bubbling into CO 2 , CO 2 The final molar concentration reaches 18% of the ionic liquid [EMIM] Ac, and the solution is obtained.

[0020] 2) Carbon dioxide assisted enzymatic hydrolysis: Add 5kg of water to the dissolution solution to make the final concentration of ionic liquid [EMIM]Ac 10.62% (w / w), then add 70,000 FPU cellulase, mix well and stir, and keep the enzyme hydrolyzed at 50°C.

[0021] 3) Microaerobic fermentation to produce ethanol: After enzymolysis, adjust the pH to 5.0, add 3% (v / v) inoculation to the enzymatic hydrolysate to inoculate Candida sheuhata to stop CO 2 , Sealed fermentation for 24h...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for converting straw to ethanol by auxiliary carbon dioxide enzymolysis and coupled fermentation in a solvent. The method comprises the following steps: treating the straw with the solvent, introducing carbon dioxide, adding enzyme for dissolution, performing efficient in situ enzymolysis on the straw in a solvent system so as to degrade the straw into sugar, adjusting the pH value, and adding microbe for in situ fermentation so as to convert sugar into ethanol. After ethanol is extracted, salt is added to prepare a double water phase system, carbon dioxide can promote recycling of the solvent, and the straw solvent recycling rate can reach 99.5%. The method can convert the straw into bioethanol in an environment-friendly and efficient manner, the solvent, the enzyme and the microbe can almost totally be recycled for reutilization, no waste is generated in the whole system, the cost is low, and the method is suitable for large-scaled application.

Description

technical field [0001] The invention relates to a method for producing ethanol by coupling carbon dioxide-assisted enzymolysis and fermentation to convert straws into ethanol, in particular to a method for synchronously recovering and purifying an ionic liquid in a mixture solution. Background technique [0002] Since cellulose is insoluble in common solvents, its solvents and dissolution methods are very important topics in the cellulose industry and basic research. The solvent of cellulose can directly dissolve cellulose to achieve the purpose of pretreatment and retain the characteristics of natural cellulose to the greatest extent. It has long been hoped that cellulose can be directly dissolved in common solvents to become a transparent solution, and then processed into various products such as silk, film, filler, non-woven fabric, etc. Cellulose solvents are divided into non-derivatized solvents and derivatized solvents, and can also be divided into aqueous phase solve...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P7/10
CPCY02E50/10
Inventor 李斌李强冯圆圆季更生屠洁李天程赵翔
Owner JIANGSU UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap