A large yellow croaker swim bladder antioxidant collagen peptide and its preparation method and application
The technology of fish bladder collagen and large yellow croaker is applied in the field of fish active antioxidant collagen peptide and its preparation, which can solve the problem that the antioxidant active collagen peptide has not been reported, and achieve the effect of remarkable antioxidant effect and no toxic and side effects.
Active Publication Date: 2017-05-10
湛江千护宝生物有限公司
View PDF2 Cites 2 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
[0003] However, the applicant found that the enzymatic hydrolysis technology to prepare large yellow croaker swim bladder collagen peptides using large yellow croaker swim bladders as raw materials is still in a blank stage, and the preparation of antioxidant active collagen peptides and their applications using fish swim bladder enzymatic hydrolysis products as materials is even more important. No report
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0031] 1) Wash the swim bladder of large yellow croaker with tap water, homogenize it with a high-speed tissue grinder, and add Na 2 HPO 4 -NaH 2 PO 4 buffer solution, adjust the pH value to 7.0, and incubate at 45°C for 10 min; add neutral protease (enzyme activity ≥ 5.0×10 5 U / g), hydrolyzed at 45 ℃ for 3 h;
[0032] 2) Adjust the pH value of the above enzymatic solution to 8.0, and add trypsin according to 1.0% of the wet weight of the swim bladder (enzyme activity ≥ 1.85×10 6 U / g), hydrolyzed at 45 ℃ for 3 h;
[0033] 3) Heat the enzymatic hydrolysis solution to 90 ℃ for 15 minutes, centrifuge at 5000 r / min for 15 minutes, and take the supernatant; the supernatant is subjected to ultrafiltration and chromatography in sequence to obtain collagen peptides.
[0034] ①Ultrafiltration: adjust the enzymatic hydrolysis supernatant to pH 7.0, use a 3 kDa ultrafiltration membrane for ultrafiltration treatment at a working pressure of 0.15 MPa and a working temperature of 30 ...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
| Property | Measurement | Unit |
|---|---|---|
| molecular weight | aaaaa | aaaaa |
Login to View More
Abstract
The invention discloses a pseudosciaena crocea swim bladder antioxidant collagen peptide and a preparation method and application thereof. The amino acid sequence of the collagen peptide is Phe-Arg-Gly-Thr-Ile-Gly-Leu(SEQ ID No:1), and the molecular weight is 649.72Da([M+H]+650.53Da) according to the ESI-MS detection. The preparation method comprises the steps of cleaning the pseudosciaena crocea swim bladder with tap water; homogenizing with a high-speed tissue pounding machine; adding a Na2HPO4-NaH2PO4 buffer solution according to a feed liquid ratio of 1g:(10-15mL), adjusting the pH value to 6.5-7.5, and preserving heat at 40-50 DEG C for 10-15min; adding neutral protease accounting for 1.0-1.5% of the wet weight of the swim bladder; performing enzymolysis at 40-50 DEG C for 2-4h; adjusting the pH value of the enzymolysis liquid to 7.5-8.0; adding trypsin accounting for 0.8-1.0% of the wet weight of the swim bladder, and performing enzymolysis at 40-50 DEG C for 2-4h; heating the enzymolysis liquid to 90-95 DEG C, and inactivating for 10-15min; centrifuging for 15-20min at 4,500-5,000r / min, and fetching the supernate; sequentially performing ultrafiltration and chromatography of the supernate to obtain collagen peptide. In the invention, the preparation process is scientific and reasonable, the enzymolysis process is easy to control, and the prepared collagen peptide has the advantages of high antioxidant activity, easiness in digestive absorption, safety, no toxic or side effects and the like, and can be used as medicines, healthcare foods and food additives.
Description
technical field [0001] The invention relates to a fish active antioxidant collagen peptide and its preparation method and application, in particular to a large yellow croaker swim bladder antioxidant collagen peptide and its preparation method and application. Background technique [0002] Fish maw, commonly known as fish paw, is mainly composed of collagen, mucopolysaccharides, and contains a variety of vitamins and trace elements such as calcium, zinc, iron, and selenium. The collagen contained in the swim bladder is easy to be absorbed and utilized by the human body. It can better improve the nutritional status of human tissue and accelerate metabolism. It is a high-quality raw material for human body to supplement and synthesize protein. [0003] However, the applicant found that the enzymatic hydrolysis technology to prepare large yellow croaker swim bladder collagen peptides using large yellow croaker swim bladders as raw materials is still in a blank stage, and the pr...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06C07K1/36C07K1/34C07K1/20C07K1/18C07K1/16C12P21/06A61P39/06A23L33/00A23L33/18
Inventor 迟长凤王斌陈荫胡发远
Owner 湛江千护宝生物有限公司