Unlock instant, AI-driven research and patent intelligence for your innovation.

A Molecular Marker Linked to Maize Photoperiod Sensitivity Main Effect QTL and Its Application

A molecular marker and photoperiod technology, applied in DNA/RNA fragments, recombinant DNA technology, etc., can solve problems such as difficulty in molecular genetic analysis, achieve fast and efficient detection, improve breeding and selection efficiency, and concise structure.

Inactive Publication Date: 2016-08-17
HENAN UNIV OF SCI & TECH
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the photoperiod response is completed by the interaction of genes involved in the developmental control of floral initiation, circadian clock regulation, and photoreceptor signal transduction, and represents the genetic characteristics of quantitative traits, which makes the molecular genetic analysis of photoperiod sensitivity very difficult. difficulty

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A Molecular Marker Linked to Maize Photoperiod Sensitivity Main Effect QTL and Its Application
  • A Molecular Marker Linked to Maize Photoperiod Sensitivity Main Effect QTL and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1. Application of Molecular Marker SSR119 and Molecular Marker SSR1336 in the Establishment of Maize Near-Isogenic Lines Containing Maize Photoperiod Sensitivity Major Gene Loci

[0036] The basic population used in the experiment comes from a warm hybrid combination: Huangzao 4 and CML288, Huangzao 4 is a Chinese temperate maize inbred line, which is not sensitive to photoperiod response; CML288 is from the Mexican International Maize and Wheat Improvement Center, selected from Pop24 The inbred line of tropical durum maize showed strong photoperiod sensitivity in temperate zone. In the present invention, the tropical inbred line CML288 which is sensitive to photoperiod is used as the donor parent, and the temperate inbred line Huangzao 4 which is insensitive to photoperiod is used as the recurrent parent. Select the method of combining, through crossing, backcrossing or selfing, to breed near-isogenic lines containing only the main gene loci of maize photoperio...

Embodiment 2

[0037] Example 2, Application of Molecular Marker SSR119 and Molecular Marker SSR1336 in Maize Molecular Breeding

[0038] Use the marker SSR119 and molecular marker SSR1336 of the present invention to carry out molecular marker detection on each corn individual plant of the warm hybrid offspring, if the genotype of the molecular marker of the measured individual corn plant is consistent with the band type of the temperate parent, the individual plant is suitable for Temperate climatic conditions can be used for further research on maize; if the measured genotype of the molecular markers of a single maize plant is consistent with the band type of the tropical parent, the individual plant is sensitive to the phenotype of the temperate climatic condition, indicating that the single plant cannot It grows and develops normally under temperate conditions, so the individual plant has no further use value and should be eliminated; if the genotype of the molecular marker of the measure...

Embodiment 3

[0039] Example 3, Application of Molecular Marker SSR119 in Molecular Breeding of Warm Hybrid Combination Maize

[0040] Use the primers of the molecular marker SSR119 of the present invention to perform PCR amplification on the DNA of a single corn plant, and carry out molecular marker detection on the amplified product. If the molecular marker is detected, it indicates that the photoperiod sensitivity is strong, and if it is not detected, it indicates that the photoperiod is sensitive. Weaker. Further, the PCR amplification product of individual plant DNA of temperate parent maize, tropical parent maize and warm heterozygous F1 can be used as a control; wherein the genomic DNA of tropical parent maize is determined to contain maize photoperiod sensitive genes; the genomic DNA of temperate parent maize is not Contains maize photoperiod sensitivity genes; if the genotype of the measured PCR amplification product of a single maize plant is consistent with the band type of the t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A molecular marker linked to the main effect QTL of maize photoperiod sensitivity, providing two SSR molecular markers SSR119 and molecular marker SSR1336 closely linked to the main effect gene of maize photoperiod sensitivity, and primers for amplifying the molecular markers SSR119 and SSR1336 Right; the method for judging the photoperiod sensitivity of maize by the molecular marker linked to the main effect QTL of maize photoperiod sensitivity is to use the total DNA of maize as a template, and use the primer sequence of the molecular marker SSR119 and the primer sequence of the molecular marker SSR1336 PCR amplification, the amplified product is detected by gel electrophoresis to determine whether the product has the same target band as the molecular markers SSR119 and SSR1336, so as to determine whether corn has a corn photoperiod sensitive gene, and then determine its photoperiod sensitivity Size: The molecular marker of the present invention can be used for genetic structure analysis, genetic map construction, gene positioning, construction of near-isogenic lines, and molecular marker-assisted selection breeding for maize photoperiod sensitivity.

Description

technical field [0001] The invention relates to a molecular marker, in particular to a molecular marker linked with the main effect QTL of maize photoperiod sensitivity and its application. Background technique [0002] Tropical maize has a broad genetic basis, rich genetic diversity in plant traits, ear traits, and stress resistance, and has little exchange with temperate germplasm. It is a rare heterologous germplasm for maize breeding in temperate regions. The use of tropical maize germplasm will undoubtedly inject new vitality into maize breeding in temperate regions. However, tropical maize has strong photoperiod sensitivity, which severely limits the utilization of tropical maize in temperate regions. [0003] Photoperiod phenomenon is a ubiquitous phenomenon in nature, and the molecular mechanism of photoperiod affecting flowering of higher plants is a hot and difficult point in developmental biology research. So far, some progress has been made in the study of the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 王翠玲杨敏李雪林韩赞平王彬胡文花
Owner HENAN UNIV OF SCI & TECH