Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of extraction method of natural high-content selenium source rice peptide

An extraction method and a high-content technology, which are applied in protein food processing, plant protein processing, food science, etc., can solve the problems of low organic selenium content, serious environmental pollution, low selenium activity, etc., and achieve the improvement of organic selenium content and technological process Simple and low-cost effect

Active Publication Date: 2016-03-30
INST OF APPLIED CHEM JIANGXI ACAD OF SCI
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The problem to be solved by the present invention is that the existing selenium-enriched rice peptide extraction method has the defects of low selenium activity, low organic selenium content, serious environmental pollution, and high energy consumption. It provides a natural high-content selenium source rice peptide extraction method, which Use ultrasonic extractor to extract directly with water without destroying the activity of selenium, the product has high content of organic selenium and will not cause environmental pollution; low energy consumption and low cost

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of extraction method of natural high-content selenium source rice peptide
  • A kind of extraction method of natural high-content selenium source rice peptide
  • A kind of extraction method of natural high-content selenium source rice peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The extraction method of natural high-content selenium source rice peptide of the present invention, it comprises the steps:

[0024] Weigh the crushed and sieved natural selenium-enriched rice as raw material, add distilled water 10 times the weight of the raw material, add 30U / mg flavored protease, 40U / mg compound protease, 25U / mg alkaline protease, 40U / mg papain, put it into an ultrasonic extractor, enzymatically hydrolyze for 4 hours under the respective optimum temperature and pH conditions, raise the temperature of each enzymolyzed solution to 100°C, keep it warm for 10min to inactivate the enzyme; centrifuge the enzymolyzed solution and filter , measure the selenium content in each filtrate, and determine the best enzyme type is flavor protease.

[0025] Take crushed and sieved natural selenium-enriched rice as raw material, add distilled water 9-12 times the weight of raw material, add 5000U active flavor protease according to 2%-4% of raw material weight, put ...

Embodiment 2

[0031] The extraction method of natural high-content selenium source rice peptide of the present invention, it comprises the steps:

[0032] Weigh 10 g of crushed and sieved natural selenium-enriched rice as raw material, add distilled water 10 times the weight of the raw material, add 30 U / mg flavored protease, 40 U / mg compound protease, 25 U / mg alkaline protease, and 5000 U enzyme activity respectively. Put 40U / mg papain into an ultrasonic extractor, and perform enzymolysis for 4 hours under the respective optimum temperature and pH conditions, raise the temperature of each enzymolysis solution to 100°C, and keep it warm for 10 minutes to inactivate the enzyme; centrifuge the enzymolysis solution Filter and measure the selenium content in the respective filtrates, see Table 1, because the selenium content is the highest after the flavor protease enzymolysis, determine the best enzyme type is the flavor protease.

[0033] Weigh 1 kg of crushed and sieved natural selenium-enri...

Embodiment 3

[0047] The extraction method of natural high-content selenium source rice peptide of the present invention, it comprises the steps:

[0048] Weigh the crushed and sieved natural selenium-enriched rice as raw material, add distilled water 10 times the weight of the raw material, add 30U / mg flavored protease, 40U / mg compound protease, 25U / mg alkaline protease, 40U / mg papain, put it into an ultrasonic extractor, enzymatically hydrolyze for 4 hours under the respective optimum temperature and pH conditions, raise the temperature of each enzymolyzed solution to 100°C, keep it warm for 10min to inactivate the enzyme; centrifuge the enzymolyzed solution and filter , Measuring the selenium content in the respective filtrates, see Table 1, determine that the best enzyme type is flavor protease.

[0049] Weigh 1kg of crushed and sieved natural selenium-enriched rice as raw material, add 12kg of distilled water, add 35g of 5000U active flavor protease, put it into an ultrasonic extracto...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to the field of feed additives of selenium-sourced rice peptide extraction technology, and specifically discloses a method for extracting natural high-content selenium-sourced rice peptide, comprising the following steps: weighing crushed and sieved selenium-enriched rice, adding rice weight 9- 12 times of distilled water, add 2%-4% of rice weight flavor protease, put it in an ultrasonic extractor for 4-6h, and keep the pH of the reaction system at 7-8 during the enzymolysis ； After the enzymolysis is completed, heat the obtained enzymolysis solution to 90-100°C and keep it warm for 5-10 minutes to inactivate the enzyme; the enzymolysis solution is centrifuged, and the filtrate is separated and purified by membrane separation technology to obtain a concentrated solution; the concentrated solution is spray-dried to obtain high Content Selenium Source Rice Peptides. The invention does not add acid-base solution, maintains the activity of selenium, increases the content of organic selenium in the product, and does not pollute the environment at the same time; uses membrane separation technology to separate and purify the enzymatic hydrolyzate, with simple process and low cost.

Description

technical field [0001] The invention relates to the technical field of extraction of selenium-sourced rice peptides, in particular to a method for extracting natural high-content selenium-sourced rice peptides. Background technique [0002] Selenium has various pharmacological effects such as anti-cancer, heart protection, anti-hepatic necrosis, prevention and treatment of myopia and cataract, detoxification, improving immunity, delaying aging and enhancing reproductive function. It is known as "the fire of life", "the patron saint of the heart" and "King of Cancer". More than 40 countries and regions in the world are selenium deficient areas. my country is a big selenium-deficient country. According to the "Atlas of Local Diseases and Environmental Factors of the People's Republic of China", there is a low-selenium zone in the area from the three northeastern provinces to the Yunnan-Guizhou Plateau, which accounts for 72% of my country's land area, of which 30% is Severe s...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A23J3/14A23J3/34
Inventor 胡居吾李雄辉熊伟付建平朱仁果韩晓丹王慧宾
Owner INST OF APPLIED CHEM JIANGXI ACAD OF SCI