Morinda officinalis tissue culture breeding method

A tissue culture, Morinda officinalis technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of low seedling rate, difficulty in artificial breeding of Morinda officinalis, etc., and achieve the effect of convenient material acquisition

Inactive Publication Date: 2014-09-17
黄振忠
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Purpose of the invention: the purpose of the invention is to provide a method for raising seedlings by tissue culture for the difficulty of artificial breeding of Morinda officinalis in the prior art and the lo

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0019] Embodiment 1: a kind of Morinda officinalis tissue culture seedling, its concrete steps are as follows:

[0020] 1. For seed collection, select robust, pest-free wild Morinda officinalis plants as the mother plant for seed collection. After the frost and solar term, the seeds can be harvested when they are mature;

[0021] 2. For the cultivation of sterile seedlings, the seeds of Morinda officinalis were soaked in 0.2% mercuric solution for 15 minutes, and then washed with sterile water for 5 times on the ultra-clean workbench for 3 minutes each time. Sow in medium and cultivate under natural scattered light indoors;

[0022] 3. Induction medium, with MS as the basic medium, adding plant hormones for induction culture, plant hormones with benzyl purine (BA) and naphthalene acetic acid (NAA), the concentration of benzyl purine (BA) is 1.0 / L, naphthalene acetic acid (NAA) concentration is 0.1mg / L, 2.5% sucrose and 0.7% agar are added at the same time, the medium temperat...

Example Embodiment

[0027] Embodiment 2: a kind of Morinda officinalis tissue culture seedling, its concrete steps are as follows:

[0028] 1. For seed collection, select robust, pest-free wild Morinda officinalis plants as the mother plant for seed collection. After the frost and solar term, the seeds can be harvested when they are mature;

[0029] 2. For the cultivation of sterile seedlings, the seeds of Morinda officinalis were soaked in 0.2% mercuric solution for 15 minutes, and then washed with sterile water for 5 times on the ultra-clean workbench for 3 minutes each time. Sow in medium and cultivate under natural scattered light indoors;

[0030] 3. Induction medium, MS was used as the basic medium, and different concentrations of plant hormones were added for induction culture. The plant hormones were benzylpurine (BA) and naphthalene acetic acid (NAA), and the concentration of benzylpurine (BA) was 2.0mg / L, the concentration of naphthalene acetic acid (NAA) is 0.2mg / L, 2.5% sucrose and ...

Example Embodiment

[0035] Embodiment 3: a kind of Morinda officinalis tissue culture seedling, its concrete steps are as follows:

[0036] 1. For seed collection, select robust, pest-free wild Morinda officinalis plants as the mother plant for seed collection. After the frost and solar term, the seeds can be harvested when they are mature;

[0037] 2. For the cultivation of sterile seedlings, the seeds of Morinda officinalis were soaked in 0.2% mercuric solution for 15 minutes, and then washed with sterile water for 5 times on the ultra-clean workbench for 3 minutes each time. Sow in medium and cultivate under natural scattered light indoors;

[0038] 3. Induction medium, MS was used as the basic medium, and different concentrations of plant hormones were added for induction culture. The plant hormones were benzylpurine (BA) and naphthalene acetic acid (NAA), and the concentration of benzylpurine (BA) was 2.5mg / L, the concentration of naphthalene acetic acid (NAA) is 0.3mg / L, 2.5% sucrose and ...

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PUM

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Abstract

The invention provides a tissue culture breeding method aiming at the problems of high artificial morinda officinalis breeding difficulty and low seedling rate. The tissue culture breeding method specifically comprises the steps of seed harvesting, germfree seedling culture, culture medium induction, cluster bud induction, subculture cluster bud multiplication, rooting induction, transplanting of test-tube seedlings and the like. The tissue culture breeding method has the beneficial effects that materials easily available, the cluster bud induction rate is 50-80 percent, the seedling rate is over 85 percent, and medicinal components of morinda officinalis can be retained; after being transplanted, the morinda officinalis grows fast, and a requirement of large-area artificial cultivation on morinda officinalis seedlings can be met.

Description

technical field [0001] The invention relates to the cultivation technology of Chinese herbal medicinal materials, in particular to a method for growing seedlings of Morinda officinalis officinalis, in particular to a method for cultivating seedlings of Morinda officinalis through tissue culture, and belongs to the technical field of cultivation of Chinese medicinal materials. Background technique [0002] Morinda officinalis (scientific name: Morinda officinalis How), is a dicotyledonous plant Rubiaceae (Rubiaceae), twining vines, opposite leaves, membranous, oblong, 3-13cm long, 1.5-5cm wide, short and acuminate at the front end, The base is blunt or round, mainly distributed in Guangdong and Guangxi, and belongs to medicinal plants. [0003] The medicinal part of Morinda officinalis is the root, which is flat cylindrical and slightly curved. It is mainly used for impotence, spermatorrhea, cold uterine infertility, irregular menstruation, cold pain in the lower abdomen, rhe...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 黄振忠
Owner 黄振忠
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