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Morinda officinalis tissue culture breeding method

A tissue culture, Morinda officinalis technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of low seedling rate, difficulty in artificial breeding of Morinda officinalis, etc., and achieve the effect of convenient material acquisition

Inactive Publication Date: 2014-09-17
黄振忠
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Purpose of the invention: the purpose of the invention is to provide a method for raising seedlings by tissue culture for the difficulty of artificial breeding of Morinda officinalis in the prior art and the low seedling rate , improve the seedling rate of Morinda officinalis, and can maintain the medicinal ingredients of Morinda officinalis, grow fast after transplanting, and can be artificially cultivated on a large scale

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: a kind of Morinda officinalis tissue culture seedling cultivation, its concrete steps are as follows:

[0020] 1. For seed collection, choose a strong, disease-free wild Morinda officinalis plant as the mother plant for seed collection. After the frost solar term passes every year, the seeds are mature and you can collect seeds;

[0021] 2. For the cultivation of sterile seedlings, put the seeds of Morinda officinalis in 0.2% mercuric chloride solution for 15 minutes, soak them in sterile water for 5 times on the ultra-clean workbench, and soak them for 3 minutes each time, and then put the seeds Sow in the culture medium and cultivate under the natural scattered light in the room;

[0022] 3. Induction medium, using MS as the basic medium, adding phytohormones for induction culture, phytohormones use benzylpurine (BA) and naphthalene acetic acid (NAA), the concentration of benzylpurine (BA) is 1.0 / L, and naphthalene acetic acid (NAA) concentration is 0.1...

Embodiment 2

[0027] Embodiment 2: a kind of Morinda officinalis tissue culture seedling cultivation, its concrete steps are as follows:

[0028] 1. For seed collection, choose a strong, disease-free wild Morinda officinalis plant as the mother plant for seed collection. After the frost solar term passes every year, the seeds are mature and you can collect seeds;

[0029] 2. For the cultivation of sterile seedlings, put the seeds of Morinda officinalis in 0.2% mercuric chloride solution for 15 minutes, soak them in sterile water for 5 times on the ultra-clean workbench, and soak them for 3 minutes each time, and then put the seeds Sow in the culture medium and cultivate under the natural scattered light in the room;

[0030] 3. Induction medium, using MS as the basic medium, adding different concentrations of phytohormones for induction culture, phytohormones use benzylpurine (BA) and naphthalene acetic acid (NAA), the concentration of benzylpurine (BA) is 2.0mg / L, the concentration of na...

Embodiment 3

[0035] Embodiment 3: a kind of Morinda officinalis tissue culture grows seedlings, and its concrete steps are as follows:

[0036] 1. For seed collection, choose a strong, disease-free wild Morinda officinalis plant as the mother plant for seed collection. After the frost solar term passes every year, the seeds are mature and you can collect seeds;

[0037] 2. For the cultivation of sterile seedlings, put the seeds of Morinda officinalis in 0.2% mercuric chloride solution for 15 minutes, soak them in sterile water for 5 times on the ultra-clean workbench, and soak them for 3 minutes each time, and then put the seeds Sow in the culture medium and cultivate under the natural scattered light in the room;

[0038] 3. Induction medium, using MS as the basic medium, adding different concentrations of phytohormones for induction culture, phytohormones use benzylpurine (BA) and naphthalic acid (NAA), the concentration of benzylpurine (BA) is 2.5mg / L, the concentration of naphthalene...

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Abstract

The invention provides a tissue culture breeding method aiming at the problems of high artificial morinda officinalis breeding difficulty and low seedling rate. The tissue culture breeding method specifically comprises the steps of seed harvesting, germfree seedling culture, culture medium induction, cluster bud induction, subculture cluster bud multiplication, rooting induction, transplanting of test-tube seedlings and the like. The tissue culture breeding method has the beneficial effects that materials easily available, the cluster bud induction rate is 50-80 percent, the seedling rate is over 85 percent, and medicinal components of morinda officinalis can be retained; after being transplanted, the morinda officinalis grows fast, and a requirement of large-area artificial cultivation on morinda officinalis seedlings can be met.

Description

technical field [0001] The invention relates to the cultivation technology of Chinese herbal medicinal materials, in particular to a method for growing seedlings of Morinda officinalis officinalis, in particular to a method for cultivating seedlings of Morinda officinalis through tissue culture, and belongs to the technical field of cultivation of Chinese medicinal materials. Background technique [0002] Morinda officinalis (scientific name: Morinda officinalis How), is a dicotyledonous plant Rubiaceae (Rubiaceae), twining vines, opposite leaves, membranous, oblong, 3-13cm long, 1.5-5cm wide, short and acuminate at the front end, The base is blunt or round, mainly distributed in Guangdong and Guangxi, and belongs to medicinal plants. [0003] The medicinal part of Morinda officinalis is the root, which is flat cylindrical and slightly curved. It is mainly used for impotence, spermatorrhea, cold uterine infertility, irregular menstruation, cold pain in the lower abdomen, rhe...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 黄振忠
Owner 黄振忠
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