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A method for ultrasonic-assisted two-phase extraction of alliin

An ultrasonic-assisted, two-phase technology, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve the problems of low cost of inorganic salts/lower alcohols, high price of phase-forming polymers, and unreasonable economics , to achieve the effect of low cost, easy scale-up and continuous operation, and high purity

Active Publication Date: 2016-08-17
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The traditional polymer / polymer aqueous two-phase system has good separation performance, but the phase-forming polymers used to construct the two-phase aqueous system are relatively expensive. For general biological products, the separation cost is too high, from Economically unreasonable and with high interfacial tension; inorganic salts / lower alcohols are gaining popularity due to their low cost, low viscosity and easy recycling

Method used

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  • A method for ultrasonic-assisted two-phase extraction of alliin
  • A method for ultrasonic-assisted two-phase extraction of alliin
  • A method for ultrasonic-assisted two-phase extraction of alliin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Prepare a two-phase aqueous system. Get the ammonium sulfate that mass fraction is 16%, add the Buri Tan-Robinson buffer solution that pH is 2.35 to dissolve, add the n-propanol of 20% (w / w), be prepared as two-phase extractant;

[0035](2) Ultrasonic-assisted aqueous two-phase extraction. In the two-phase system obtained in step (1), add 2% precisely weighed garlic powder (the source of the garlic powder: the garlic slices are crushed and passed through a 60-mesh sieve, and the garlic slices used are conventional commercially available products. The ultrasonic conditions were set as power 540W, temperature 30°C, and time 40min, and ultrasonic-assisted aqueous two-phase extraction was carried out. After the extraction, the alliin extract with obvious phase was separated by centrifugation.

[0036] Measure the volume of the upper and lower phases of the aqueous two-phase, measure the concentration of alliin in the upper and lower phases of the two-aqueous phase by h...

Embodiment 2

[0042] (1) Preparation of a two-phase aqueous system Take ammonium sulfate with a mass fraction of 19%, add a Beritan-Robinson buffer solution with a pH of 2.35 to dissolve, add 23% n-propanol, and prepare a two-phase aqueous extractant;

[0043] (2) Ultrasonic-assisted aqueous two-phase extraction Add 2% (w / w) garlic powder to the aqueous two-phase system obtained in step (1) (source of garlic powder: crush garlic slices and pass through a 60-mesh sieve, the resulting The garlic slices used are conventional commercially available products), and the ultrasonic conditions were set to power 540W, extraction temperature 30°C, extraction time 40min, and ultrasonic-assisted two-phase extraction was performed. . The extraction rate of the obtained alliin was 20.6 mg / g.

Embodiment 3

[0045] (1) Preparation of a two-phase aqueous system Take ammonium sulfate with a mass fraction of 28%, add Beritan-Robinson buffer solution with a pH of 2.35 to dissolve, and then add 17% n-propanol to prepare a two-phase aqueous extractant;

[0046] (2) Ultrasonic-assisted aqueous two-phase extraction Add 2% (w / w) garlic powder to the aqueous two-phase system obtained in step (1) (source of garlic powder: crush garlic slices and pass through a 60-mesh sieve, the resulting The garlic slices used are conventional commercially available products), and the ultrasonic conditions were set to power 540W, extraction temperature 30°C, extraction time 40min, and ultrasonic-assisted two-phase extraction was performed. . The extraction rate of the obtained alliin was 19.2 mg / g.

[0047] The selection of different two-phase systems for extracting alliin in table 1

[0048]

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Abstract

The invention belongs to the field of deep processing of garlic, and particularly discloses a method for extracting alliin in an ultrasonic-assisted two-phase aqueous phase. The method comprises the following steps: (1) Take inorganic salt, add Beritan-Robinson buffer solution to dissolve, add organic solvent, mix and stand for phase separation to obtain two-phase extractant; (2) Adjust two-phase extractant The pH of the solution is 2.0‑4.0. Garlic powder is added to the two-phase extractant, and ultrasonic waves are used for auxiliary extraction. After extraction, it is taken out and centrifuged and left to separate the phases. The yield of alliin. The invention has high extraction yield and higher purity of alliin, adopts small molecular organic solvent with small interfacial tension, contributes to mass transfer between two phases, low cost, simple equipment, simple operation, and post-treatment of the extract Simple, easy to scale up and continuous operation.

Description

(1) Technical field [0001] The invention belongs to the field of intensive processing of garlic, and in particular relates to a method for extracting alliin in an ultrasonic-assisted two-phase aqueous phase. (2) Background technology [0002] Garlic has been widely used in the treatment and prevention of some diseases. It has been found that the sulfur compounds in it have good medical effects, especially in the prevention of cancer. One of the flavor precursors, alliin (S-allyl -L cysteine ​​sulfoxide) has a preventive effect. Studies have shown that alliin has anti-peroxide, anti-diabetes, anti-oxidant, anti-bacterial, anti-tumor and anti-cancer effects; alliin is unstable, and when raw garlic is crushed or chopped, enzymes will occur Accelerate the reaction to generate allicin, which also has antibacterial, anti-tumor, and immunomodulatory effects, but allicin is easy to decompose and difficult to store, and the medical and health properties of alliin are not much differ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C317/48C07C315/06
Inventor 崔波姜秀敏檀琮萍卢艳敏
Owner QILU UNIV OF TECH