A preparation method of composite nano-silver film for DNA fluorescence detection
A fluorescence detection and nano-silver technology, applied in fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of cumbersome preparation, insensitive detection, low fluorescence intensity, etc., to enhance fluorescence intensity, improve detection sensitivity, and biocompatibility. Good results
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0025] (1) Immerse the quartz plate in a mixed solution of hydrogen peroxide and concentrated sulfuric acid with a volume ratio of 3:7, keep it for 1 hour, take it out, rinse it with deionized water and dry it with nitrogen gas for use.
[0026] (2) Immerse the above-mentioned quartz sheet in a 1 mg / mL positively charged chitosan solution for 20 minutes, take it out and rinse it with deionized water, and dry it with nitrogen; then keep it immersed in a 1 mg / mL negatively charged sodium alginate solution After 20 minutes, it was taken out, rinsed with deionized water, and dried with nitrogen; the above process was repeated 4 times to prepare a four-layer chitosan / sodium alginate self-assembled multilayer film.
[0027] (3) Immerse the self-assembled multilayer film prepared in step (2) in a pre-prepared silver ammonia solution with a mass percentage of 1%, heat up to a certain temperature range of 80° C. and keep stirring for 1 hour to prepare a composite nano-silver film .
...
Embodiment 2
[0032] (1) Dip the silicon wafer in a mixed solution of hydrogen peroxide and concentrated sulfuric acid with a volume ratio of 3:7, keep it for 1 hour, take it out, rinse it with deionized water and dry it with nitrogen gas for use.
[0033] (2) Immerse the above-mentioned silicon wafer in 1 mg / mL positively charged chitosan solution for 20 minutes, take it out and rinse it with deionized water, and dry it with nitrogen; then keep it immersed in 1 mg / mL negatively charged sodium alginate solution After 20 minutes, it was taken out, rinsed with deionized water, and dried with nitrogen; the above process was repeated 4 times to prepare a four-layer chitosan / sodium alginate self-assembled multilayer film.
[0034] (3) Immerse the self-assembled multilayer film prepared in step (2) in a pre-prepared silver ammonia solution with a mass percentage of 1%, heat up to a certain temperature range of 80° C. and keep stirring for 1 hour to prepare a composite nano-silver film .
[0035]...
Embodiment 3
[0039] (1) Immerse the quartz plate in a mixed solution of hydrogen peroxide and concentrated sulfuric acid with a volume ratio of 3:7, keep it for 1 hour, take it out, rinse it with deionized water and dry it with nitrogen gas for use.
[0040] (2) Immerse the above-mentioned quartz plate in 2 mg / mL positively charged polypropylene ammonium chloride solution for 20 minutes, take it out and rinse it with deionized water, and blow it dry with nitrogen; then immerse it in 2 mg / mL negatively charged alginic acid After keeping the sodium solution for 20 minutes, take it out, rinse it with deionized water, and blow it dry with nitrogen; repeat the above process 3 times to prepare a three-layer polypropylene ammonium chloride / sodium alginate self-assembled multilayer film.
[0041](3) Immerse the self-assembled multilayer film prepared in step (2) in a pre-prepared silver ammonia solution with a mass percentage of 1%, heat up to a certain temperature range of 80° C. and keep stirring...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More