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Identification of poliovirus strains

A polio and virus strain technology, applied in the field of virology and virus vaccinology, can solve the problems of complex preparation of antiserum, inability to distinguish wild-type vaccine strains, time-consuming and labor-intensive problems

Inactive Publication Date: 2014-10-22
由卫生福利和体育大臣代表的荷兰王国
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  • Application Information

AI Technical Summary

Problems solved by technology

Currently, serological methods are used to identify and quantify the three poliovirus serotypes present in IPV, but they are often unable to distinguish between wild-type and attenuated vaccine strains (Westdijk et al., 2011)
Although serological assays capable of distinguishing wild-type poliovirus strains from attenuated poliovirus strains are available, they require highly specific antisera (van der Avoort et al., 1995), The preparation of such highly specific antisera is complex and time consuming (van Wezel and Hazendonk, 1979)

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  • Identification of poliovirus strains
  • Identification of poliovirus strains
  • Identification of poliovirus strains

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Embodiment Construction

[0011] The present invention relates to a method for identifying and / or differentiating poliovirus strains, especially for vaccine production. The method is based on selective hybridization using oligonucleotides (i.e., primers and / or probes), which enables the discrimination of closely related poliovirus strains based on the presence of nucleotide polymorphisms between those poliovirus strains. But not the same strain of poliovirus.

[0012] Poliovirus is an enterovirus that infects humans. Three serotypes of poliovirus are known, serotype 1, 2 and 3 or PV1, PV2 and PV3. For each serotype, virulent and attenuated poliovirus strains can be used for vaccine production (see, eg, Table 1). While it is possible for most poliovirus strains to design specific oligonucleotide primers or probes that can differentiate these strains from other poliovirus strains, for two important sera For poliovirus type 1 vaccine strains (i.e., the virulent Mahoney strain and the attenuated Sabin t...

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Abstract

The present invention relates to methods for identifying and / or distinguishing polioviral strains, in particular polioviral strains used in vaccine production. The methods are based on selective hybridisation with oligonucleotides, i.e. primers and / or probes, that allow to distinguish between closely related but different polioviral strains on the basis of nucleotidepolymorphisms existing between those polioviral strains. Preferably, the methods employ amplification or amplification-ligation assays for detecting the selective hybridisation. The invention further relates to oligonucleotides for use in the methods of the invention and kits comprising such oligonucleotides and optionally enzymes and buffers for carrying out the methods of the invention. label

Description

technical field [0001] The present invention relates to the fields of virology and viral vaccinology. In particular, the present invention relates to tools and methods for identifying vaccine-specific poliovirus strains. Background technique [0002] The use of live attenuated oral polio vaccine (OPV) and inactivated poliovirus vaccine (IPV) has significantly reduced the number of polio cases and polio outbreaks (Dutta, 2008; Crawford and Buttery, 2010). The Global Polio Eradication Initiative of the World Health Organization (WHO) is currently focused on eradicating the disease and eradicating wild polioviruses (WHO, 2003; Sutter et al., 2003). Despite great efforts towards universal vaccination, dozens of polio cases have been reported in recent years (Crawford and Buttery, 2010; Global Polio Eradication Initiative. Global polio case count. 2011. Available From: (Acquired Nov14, 2011); Kew et al., 2005). Vaccination with OPV can introduce vaccine-derived poliovirus, le...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68
CPCC12Q1/6827C12Q1/701Y02A50/30C12Q2600/158
Inventor B·梅茨O·E·M·尼斯特J·J·毛斯哈恩D·R·梅克斯
Owner 由卫生福利和体育大臣代表的荷兰王国