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EGCG (epigallocatechin gallate) purification method, EGCG obtained by same and medicinal composition prepared from EGCG

A purification method and the technology of Kampo hexagon, applied in the field of chemical purification of natural products, can solve the problems of low purification content of EGCG, large toxic and side effects of drugs, unclear drug efficacy, etc., and achieve low cost, low dosage and simple operation. Effect

Inactive Publication Date: 2014-11-05
四川天予植物药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the defects of low EGCG purification content and low yield in the prior art and in order to overcome the defects of unclear drug efficacy and large drug side effects for the treatment of Alzheimer's disease

Method used

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  • EGCG (epigallocatechin gallate) purification method, EGCG obtained by same and medicinal composition prepared from EGCG
  • EGCG (epigallocatechin gallate) purification method, EGCG obtained by same and medicinal composition prepared from EGCG
  • EGCG (epigallocatechin gallate) purification method, EGCG obtained by same and medicinal composition prepared from EGCG

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1, the EGCG purification process of the present invention are as follows:

[0020] (1) Column packing: 100 g of 70-mesh silica gel is packed in a glass chromatography column with a diameter of 5 cm and a height of 80 cm by dry method;

[0021] (2) Dissolution: Add 60ml of 70% ethanol to soak 20g tea polyphenols and pour it into a silica gel column for chromatography;

[0022] (3) Elution: Then add ethyl acetate-petroleum ether mixed solvent with a volume ratio of 3:5 for elution;

[0023] (4) Collection: Use thin layer chromatography to collect the EGCG elution fraction of each elution fraction;

[0024] (5) Concentration: Combine the EGCG collection solution and concentrate to obtain the EGCG extract;

[0025] (6) Crystallization: put the EGCG extract in a conical flask, add 140ml of 75% ethanol, then heat in a water bath at 65°C, stir while heating, dissolve for 1 hour, leave it overnight to crystallize, filter, and collect crystals;

[0026] (7) Drying...

Embodiment 2

[0027] Embodiment 2, the EGCG purification process of the present invention are as follows:

[0028] (1) Column packing: 120 g of 70-mesh silica gel is packed in a glass chromatography column with a diameter of 5 cm and a height of 80 cm by dry method;

[0029] (2) Dissolution: Add 70ml of 70% ethanol to soak 20g of tea polyphenols and pour it into a silica gel column for chromatography;

[0030] (3) Elution: Then add ethyl acetate-petroleum ether mixed solvent with a volume ratio of 4:6 for elution;

[0031] (4) Collection: Use thin layer chromatography to collect the EGCG elution fraction of each elution fraction;

[0032] (5) Concentration: Combine the collected EGCG solution and then concentrate to obtain EGCG extract;

[0033] (6) Crystallization: put the EGCG extract in a conical flask, add 160ml of 75% ethanol, then heat in a water bath at 65°C, stir while heating, dissolve for 1 hour, leave it overnight to crystallize, filter, and collect crystals;

[0034] (7) Dryin...

Embodiment 3

[0035] Embodiment 3, the EGCG purification process of the present invention are as follows:

[0036] (1) Column packing: 140 g of 70-mesh silica gel is packed in a glass chromatography column with a diameter of 5 cm and a height of 80 cm by dry method;

[0037] (2) Dissolution: Add 80ml of 70% ethanol to soak 20g of tea polyphenols and pour it into a silica gel column for chromatography;

[0038] (3) Elution: Then add ethyl acetate-petroleum ether mixed solvent with a volume ratio of 5:7 for elution;

[0039] (4) Collection: Use thin layer chromatography to collect the EGCG elution fraction of each elution fraction;

[0040] (5) Concentration: Combine the EGCG collection solution and concentrate to obtain the EGCG extract;

[0041] (6) Crystallization: put the EGCG extract in a conical flask, add 180ml of 75% ethanol, then heat in a water bath at 65°C, stir while heating, dissolve for 1 hour, leave to crystallize overnight, filter, and collect crystals;

[0042] (7) Drying...

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Abstract

The invention belongs to applications of medicinal compositions in the technical field of natural product chemical purification and the field of medicines, and particularly relates to an EGCG (epigallocatechin gallate) purification method, EGCG obtained by the same and a medicinal composition prepared from the EGCG. According to the method, tea polyphenol with low content of EGCG is used as a raw material, is packed to columns, and is subjected to dissolving, eluting, collecting, concentrating, crystallizing and drying. The medicinal composition composed of the prepared EGCG and tetrandrine can be applied to preparation of medicaments for treating alzheimer disease. The EGCG purification method is simple and convenient to operate, has low energy consumption, and is low in usage of a dissolving agent and an eluent, thus playing effects of low cost and environmental friendliness; the purity of the prepared EGCG is more than or equal to 99 percent, and the yield of the EGCG is more than or equal to 90 percent, so that large-scale popularization and application of EGCG can be facilitated; the EGCG medicinal composition is capable of retarding the developing process of alzheimer disease, shows a remarkable treatment effect, improves the memory restoration of a patient, does not have a side effect, and has a remarkable drug effect, thus having a wide medical prospect.

Description

technical field [0001] The invention belongs to the technical field of chemical purification of natural products and the application of pharmaceutical compositions in the pharmaceutical field, in particular to a method for purifying EGCG and the obtained EGCG and pharmaceutical compositions. Background technique [0002] EGCG, namely epigallocatechin gallate, is extracted and refined from green tea. The main component of green tea polyphenols is catechin. It also includes epicatechin (EC) / epigallocatechin gallate (EGCG), epicatechin gallate (ECG), etc., and EGCG accounts for about 50% of the total catechin. Studies have shown that EGCG has strong antioxidant and preventive functions against cancer and cardiovascular diseases, and can increase the sensitivity of cancer cells to chemotherapy, while reducing the toxicity of chemotherapy to the heart. At the same time, EGCG has anti-free radical DNA damage, anti-radiation and ultraviolet rays, prevents oil peroxidation, reduce...

Claims

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Application Information

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IPC IPC(8): C07D311/62A61K31/4748A61P25/28A61K31/353
CPCA61K31/353A61K31/4748C07D311/62A61K2300/00
Inventor 李威李选民张雄修
Owner 四川天予植物药业有限公司