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A kind of detection method of compound donkey-hide gelatin slurry oral liquid

A technology of compound donkey-hide gelatin paste and detection method, which is applied in the field of quality detection of compound traditional Chinese medicines, can solve the problems of unreasonable quality of traditional Chinese medicine compound preparations and no quantitative detection method for active chemical components.

Active Publication Date: 2016-05-25
SHAN DONG DONG E E JIAO
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Problems solved by technology

[0010] However, at present, the quality standard detection method of compound donkey-hide gelatin pulp oral liquid is limited to total flavonoids, total saponins and other indicators, there is no quantitative detection method for active chemical components, and it cannot fully reflect the quality of the product at all. The quality is not very reasonable; and the competition of similar products in the market is becoming increasingly fierce, so in order to protect the interests of patients, it is of great significance to establish a detection method for the active ingredients in compound donkey-hide gelatin oral liquid

Method used

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  • A kind of detection method of compound donkey-hide gelatin slurry oral liquid
  • A kind of detection method of compound donkey-hide gelatin slurry oral liquid
  • A kind of detection method of compound donkey-hide gelatin slurry oral liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The quality detection of embodiment 1 traditional Chinese medicine compound Ejiaojiao oral liquid

[0047] 1. Experimental materials

[0048] (1) Preparation of the test solution: Take 5 batches of Compound Ejiao syrup oral liquid (110950, 1109511, 110952, 110953, 110954), remove the protein in the Ejiao syrup according to the sample pretreatment method described in the technical scheme, and centrifuge , stand, centrifuge concentration, dilution and other steps to analyze;

[0049] (2) Preparation of standard products: Precisely weigh different amounts of chlorogenic acid, ergosteroside, isoergosteroside, codonopside, baicalin, notoginsenoside R2, 20(S)-ginsenoside Rg2, 20( R)-ginsenoside Rg2, 20(S)-ginsenoside Rg3, 20(R)-ginsenoside Rg3, and mixed standard solution as shown in the table below.

[0050] Table 1 Standard solution (μg / mL)

[0051]

[0052]

[0053] (3) Preparation of internal standard solution: 2 mg of naringin and 0.5 mg of digoxin were precisel...

Embodiment 2

[0067] Example 2 Sensitivity experiment

[0068] Sensitivity experiments include the sensitivity of the instrument and the sensitivity of the method, which are expressed by the detection limit of the instrument and the limit of quantification of the method, respectively.

[0069] Dilute HB-6 with 80% methanol-water 10 times, 30 times, 60 times, 120 times, 240 times, 360 times, and 480 times respectively. According to the sample pretreatment method, add the dilution of HB-6 to the blank gel After centrifugation, standing, centrifugation concentration, dilution and other steps, the juice was injected and analyzed, and the signal-to-noise ratio was determined. The limit of quantification of the method.

Embodiment 3

[0070] Example 3 Accuracy and Repeatability Experiment

[0071] After taking 4 pieces of the same batch of Compound E Jiaojiao oral liquid and mixing, after 20 minutes of ultrasound, accurately pipette 1 mL of oral liquid and 1 mL of 1 mL of blank gum juice. 40 μL of the internal standard solution was processed according to the standard operation, and injected for analysis, and the recovery rate and relative standard deviation were calculated; the accuracy was expressed by the recovery rate, and the repeatability was expressed by the relative standard deviation.

[0072] According to the pretreatment method and analysis method of the sample, to investigate the influence of the matrix on the analysis results, it is necessary to determine the extraction efficiency and matrix effect of the sample pretreatment. Three groups of standard stock solutions with high, medium and low concentrations were prepared, respectively, before and after protein precipitation to prepare recovered s...

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Abstract

The invention discloses a quality detection method of a compound donkey-hide gelatin slurry oral liquid. The method adopts a high performance liquid chromatography (HPLC) which is serially connected to a triple quadrupole mass spectrum (MS) to carry out on-line detection on four medicinal materials and ten compounds. The optimal mobile phase is selected through a large amount of experiments, and the gradient elution program and flow speed, MS conditions, and related compound parameters are also determined. Through the observation of methodology, the quality detection method has the advantages of good stability, selectivity, and repeatability and reliable analysis result. Moreover the sensitivity of the method is high, and the quantitative and qualitative analysis on a compound donkey-hide gelatin slurry oral liquid can be well achieved by the method. The quality of a compound donkey-hide gelatin slurry oral liquid can be objectively, comprehensively, and precisely evaluated, and the method has a very important meaning on controlling the quality of compound donkey-hide gelatin slurry oral liquid and guaranteeing the curative effect in clinic.

Description

technical field [0001] The invention relates to a quality detection method of compound traditional Chinese medicine, in particular to a quality detection method of compound Ejiao syrup oral liquid. Background technique [0002] Compound Ejiao Oral Liquid is a compound traditional Chinese medicine oral liquid produced by Shandong Dong E E E Jiao Co., Ltd., which is processed and refined from five herbs of Ejiao, Red Ginseng, Codonopsis, Rehmannia glutinosa and Hawthorn. The function of liver and kidney is mainly used for deficiency of qi and blood, dizziness, palpitations, insomnia, loss of appetite and anemia. [0003] Compound donkey-hide gelatin oral liquid is made from the following raw materials: 5 parts of donkey-hide gelatin, 6 parts of red ginseng, 8 parts of Shudi, 12 parts of Codonopsis pilosula, and 5 parts of hawthorn. The preparation method of making the above-mentioned components into the medicine of the present invention is as follows: [0004] ①Weigh the raw ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 瞿海斌周祥山田守生王春艳沈丽娟李民张路李士栋
Owner SHAN DONG DONG E E JIAO
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