Heavy metal-tolerant aerobic denitrifying strain and its application
A technology of nitrite nitrogen and microbial strains, applied in the direction of bacteria, chemical instruments and methods, and methods based on microorganisms, can solve the problems of nitrite nitrogen accumulation and secondary pollution, and achieve convenient and simple operation and excellent environment The effect of benefits and social benefits
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Embodiment 1
[0028] Isolation and Purification of Bacillus pumilus SNR‐3
[0029] Enrichment medium 1: potassium sodium tartrate 20g / L, trisodium citrate 10g / L, sodium acetate 10g / L, KNO 3 2g / L, K 2 HPO 4 0.5g / L, MgSO 4 ·7H 2O0.2g / L, trace element solution 0.2%, pH7.5. Trace element solution: EDTA-2Na57.1mg / L, ZnSO 4 ·7H 2 O3.9mg / L, CaCl 2 2H 2 O7mg / L, MnCl 2 4H 2 O5.1mg / L, FeSO 4 ·7H 2 O5.0mg / L, CuSO 4 ·5H 2 O1.6mg / L, CoC1 2 1.6mg / L.
[0030] Enrichment medium 2: Add 100mg / LCuSO4·5H2O on the basis of enrichment medium 1, 50mg / L (CH 3 COO) 2 Cd·2H 2 O.
[0031] BTB medium: KNO 3 1.0g / L,C 6 h 5 Na 3 o 2 2H 2 O1.0g / L, KH 2 PO 4 1.0 / Lg, FeS0 4 ·7H 2 00.05g / L, CaCl 2 0.2g / L, MgSO 4 ·7H 2 O1.0g, 1% BTB1mL, agar 20.0g / L, pH6.8.
[0032] Denitrification medium: potassium sodium tartrate 20g / L, KNO 3 2.5g / L, K 2 HPO 4 0.5g / L, MgSO 4 ·7H 2 O0.2g / L, agar 20.0g / L, pH7.5.
[0033] Collect activated sludge samples from the coastal tidal flats of Chongming Island, ...
Embodiment 2
[0036] Denitrification Effect of Bacillus pumilus SNR‐3 on Nitrogenous Wastewater
[0037] Seed medium: glucose 5g / L, beef extract 3g / L, peptone 5g / L, NaCl 0.5g / L, adjust pH to 6.5 with NaOH solution.
[0038] Fermentation medium: glucose 20g / L, yeast extract 2g / L, peptone 5g / L, K 2 HPO 4 ·3H 2 O2.5g / L, MgSO 4 ·7H 2 O0.1g / L, use NaOH solution to adjust pH7.0.
[0039] Bacillus pumilus SNR‐3 (CGMCCNO.7126) was cultured in the seed medium, 30°C, 200r / min shaker conditions for 10h, and the seed solution was inoculated in the pre- Cultivate in a 5L fermenter with 3.5L sterilized fermentation medium, culture conditions: 30°C, 300r / min, ventilation 1.5L / min, no need to adjust pH during fermentation. After 12 hours of fermentation, the cell concentration reached 300×10 8 cfu / mL.
[0040] The above-mentioned bacterial solution was inserted into the sewage containing the initial concentration of nitrate nitrogen of 300 mg / L and the initial concentration of nitrite nitrogen of 1...
Embodiment 3
[0044] Effect of Bacillus pumilus SNR‐3 on Cu 2+ Remediation Effect of Polluted Nitrogenous Sewage
[0045] Seed medium: glucose 5g / L, beef extract 3g / L, peptone 5g / L, MgSO 4 ·7H 2 O0.3g / L, use NaOH solution to adjust pH6.5.
[0046] Fermentation medium: glucose 15g / L, sucrose 10g / L, peptone 5g / L, beef extract 3g / L, K 2 HPO 4 ·3H 2 O2.5g / L, MgSO 4 ·7H 2 O0.1g / L, use NaOH solution to adjust pH7.2.
[0047] Bacillus pumilus SNR‐3 (CGMCCNO.7126) was cultured in the seed medium, 30°C, 200r / min shaker conditions for 10h, and the seed solution was inoculated in the pre- Cultivate in a 5L fermenter with 3.5L of sterilized fermentation medium, culture conditions: 35°C, 300r / min, ventilation rate 2.0L / min, no need to adjust pH during fermentation. After 8 hours of fermentation, the cell concentration reached 300×10 8 cfu / mL.
[0048] The above-mentioned bacterial solution was inserted into an inoculum containing 300 mg / L of nitrate nitrogen initial concentration, 50 mg / L of ...
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