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A device and method for isolating hematopoietic stem cells from umbilical cord blood

A technology for hematopoietic stem cells and umbilical cord blood is applied in the field of devices for separating hematopoietic stem cells, which can solve the problems of long time consumption, complicated operation, low efficiency and the like, and achieve the effects of simple use method, high recovery efficiency and simple device.

Active Publication Date: 2016-05-25
SHANDONG QILU STEM CELL ENG
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  • Summary
  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

At present, the preparation of hematopoietic stem cells in the cord blood bank adopts the centrifuge slurry separation method. This method requires a large centrifuge and a slurry separation clamp, which is time-consuming, complicated to operate, and inefficient.

Method used

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  • A device and method for isolating hematopoietic stem cells from umbilical cord blood
  • A device and method for isolating hematopoietic stem cells from umbilical cord blood

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Embodiment 1

[0029] The device of the present invention is formed by connecting the following components: comprising a blood collection bag 1, a plasma bag 2, a red blood cell collection bag 3, an eluent bag 4, a stem cell collection bag 5, a white blood cell filter 7 and a booster pump 8, The blood collection bag 1 is connected to the blood collection device through the first connecting pipe 91 with the 1# valve 71; the blood collection bag 1 and the plasma bag 2 are connected through the second connecting pipe 92 with the 2# valve 72, and the second connecting pipe 92 A 1# clot filter screen 61 is also provided between the 2# valve 72 and the plasma bag 2; the blood collection bag 1 and the eluent bag 4 are connected by a third connecting pipe 93, and the third connecting pipe 93 is successively provided with 3# valve 73, 2# clot filter screen 62, leukocyte filter 7 and 5# valve 75; between 2# clot filter screen 62 and leukocyte filter 7, there is a fourth connecting pipe 94 with 7# valve...

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Abstract

The invention relates to a device and a method for separating hematopoietic stem cells from cord blood. The device for separating hematopoietic stem cells from cord blood comprises a blood sampling bag, a plasma bag, an erythrocyte collecting bag, an eluant bag, a stem cell collecting bag, a leukocyte filter and a pressurizing pump, wherein a coagulum filtering net is arranged between the blood sampling bag and the plasma bag; another coagulum filtering net and the leukocyte filter are arranged between the blood sampling bag and the eluant bag; the pressurizing pump is arranged between the plasma bag and the erythrocyte collecting bag and is used for achieving reverse filling of plasma. The device is simple, the use method is simple and convenient, the recycling rate of the prepared hematopoietic stem cells is greater than 83%, the hematopoietic stem cells are high in activity, less time is spent, the recycling efficiency is high, a new method is provided for hematopoietic stem cell preparation, and relatively good practical application values and popularization values are achieved.

Description

technical field [0001] The invention relates to a device and method for separating hematopoietic stem cells from umbilical cord blood. technical background [0002] Hematopoietic stem cells (HSCs) are a type of pluripotent stem cells present in hematopoietic tissues, which can differentiate and proliferate into different blood cell lines and further generate blood cells. Hematopoietic stem cells are widely used in the treatment of blood system diseases, autoimmune diseases and some solid tumors. Hematopoietic stem cells can be divided into bone marrow hematopoietic stem cells, peripheral blood hematopoietic stem cells and umbilical cord blood hematopoietic stem cells according to their source. Compared with bone marrow and peripheral blood hematopoietic stem cells, umbilical cord blood hematopoietic stem cells are more primitive and have the strongest self-proliferation ability, and umbilical cord blood has the advantages of convenient collection, immature cell immunity, an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/12C12N5/0789
CPCC12M47/04C12N5/0647C12N2509/00
Inventor 迟令龙李栋
Owner SHANDONG QILU STEM CELL ENG
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