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Strain and microbial agent capable of reducing content of cadmium as well as preparation and application methods of strain and microbial agent

A technology of content and bacterial agent, applied in biochemical equipment and methods, methods based on microorganisms, bacteria, etc., can solve the problems of heavy metal pollution in paddy fields, poor effect of alkaline soil, unsuitable for on-site operation, etc., to achieve unsuitable Effects on agricultural production and income, low cost, and small environmental impact

Active Publication Date: 2014-12-10
HUNAN INST OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Hunan Province is the main grain producing area in the country. It has been known as the "land of fish and rice" and "the world's granary" since ancient times. It shoulders the great responsibility of ensuring national food security. The quality of "Xiangmi" has been compromised, causing great harm to the health of local residents
[0003] Currently, heavy metal-contaminated soil remediation technologies mainly include phytoremediation, electrodynamic remediation, leaching, thermal desorption, vitrification, and fixation and stabilization. Among the above heavy metal-contaminated soil remediation methods, phytoremediation requires considerable It takes a long time (decades or even hundreds of years) and there are problems with the treatment of plants; electrokinetic remediation is only suitable for small areas of pollution, and it is not easy to operate on site; the leaching method needs to add surfactants, which has the risk of polluting groundwater; thermal The desorption method is a method of recovering gas by heating, which is not suitable for on-site operation, high cost, and narrow scope of application; the vitrification method changes the properties of the soil and is only suitable for serious pollution and small areas; adding lime Stabilization methods increase soil pH, cause soil compaction, and do not work well on alkaline soils
Therefore, it is not suitable for the treatment of heavy metal pollution in large-scale paddy fields.

Method used

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  • Strain and microbial agent capable of reducing content of cadmium as well as preparation and application methods of strain and microbial agent
  • Strain and microbial agent capable of reducing content of cadmium as well as preparation and application methods of strain and microbial agent

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Embodiment 1

[0042] The present invention will be described in further detail below in conjunction with the examples, without limiting the present invention. Embodiment 1, preparation of microbial fermentation inoculant;

[0043] a. Activation of bacteria

[0044] Inoculate Bacillus polymyxa P1 on the slant of the solid seed medium, and activate and cultivate at 28-35°C for 24-36 hours;

[0045] Solid seed medium: tryptone 10g / L; yeast extract 5g / L; sodium chloride 10g / L; agar 2%; pH natural;

[0046] b. Preparation of seed solution

[0047] Inoculating Bacillus polymyxa from the slant of the solid seed culture medium into the liquid seed culture medium, shaking and culturing at 28-35°C for 24-36 hours to prepare the seed liquid;

[0048] Liquid seed medium: tryptone 10g / L; yeast extract 5g / L; sodium chloride 10g / L; pH natural;

[0049] c. Seed tank fermentation

[0050] Inoculate the seed solution obtained in step b into the seed tank with an inoculum volume percentage of 5%-8% of th...

Embodiment 2

[0057] Microbial agent WSW of the present invention and contrast CK (do not add any passivating agent) reduce the experimental result of the cadmium content of each organ of rice plant at different growth stages of rice, see figure 1 and Table 1.

[0058] The usage method of the microbial agent of the present invention is as follows: before soil preparation, the agent is evenly spread on the whole paddy field at a rate of 100 kg / mu, the field is plowed, and the soil can be planted after plowing and raking.

[0059] Table 1 The cadmium-reducing effect of the microbial inoculum product of the present invention applied on rice

[0060]

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Abstract

The invention discloses a strain and a microbial agent capable of reducing the content of cadmium as well as preparation and application methods of the strain and microbial agent. The strain is Paenibacillus polymyxa P1 of which the preservation number is CCTCC NO: M 2014319, when the microbial agent obtained by fermentation is applied to low-level and middle-level cadmium polluted paddy field, the content of cadmium in paddy rice can be effectively reduced and the microbial agent has huge application and popularization values.

Description

technical field [0001] The invention belongs to the technical field of remediation of heavy metal-contaminated soil, and in particular relates to a bacterial strain for reducing cadmium content, a bacterial agent and a preparation and use method thereof. Background technique [0002] Soil is the main material basis for human survival. However, due to the increasing mining, smelting, processing, and commercial manufacturing activities of heavy metals, many heavy metals such as lead, cadmium, and mercury enter the atmosphere, water, and soil, causing serious environmental pollution. According to statistics, about 30,000 tons of cadmium is released into the environment every year in the world, and 82%-94% of the cadmium will enter the soil. Long-term intake of rice, vegetables, and fruits seriously polluted by cadmium, and drinking water with excessive cadmium will cause symptoms of chronic poisoning, affect the nervous system, hematopoietic system, digestive system, and repro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/01
Inventor 郭照辉单世平张德元杜东霞刘前刚程伟黄军王玉双付祖姣魏小武
Owner HUNAN INST OF MICROBIOLOGY