Bacterial strain and bacterial agent for reducing cadmium content and preparation and use method thereof
A technology of content and bacterial agent, applied in biochemical equipment and methods, methods based on microorganisms, bacteria, etc., can solve the problems of heavy metal pollution in paddy fields, poor effect of alkaline soil, unsuitable for on-site operation, etc., to achieve unsuitable Effects on agricultural production and income, low cost, and small environmental impact
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Embodiment 1
[0042] The present invention will be described in further detail below in conjunction with the examples, without limiting the present invention. Embodiment 1, preparation of microbial fermentation inoculant;
[0043] a. Activation of bacteria
[0044] Inoculate Bacillus polymyxa P1 on the slant of the solid seed medium, and activate and cultivate at 28-35°C for 24-36 hours;
[0045] Solid seed medium: tryptone 10g / L; yeast extract 5g / L; sodium chloride 10g / L; agar 2%; pH natural;
[0046] b. Preparation of seed solution
[0047] Inoculating Bacillus polymyxa from the slant of the solid seed culture medium into the liquid seed culture medium, shaking and culturing at 28-35°C for 24-36 hours to prepare the seed liquid;
[0048] Liquid seed medium: tryptone 10g / L; yeast extract 5g / L; sodium chloride 10g / L; pH natural;
[0049] c. Seed tank fermentation
[0050] Inoculate the seed solution obtained in step b into the seed tank with an inoculum volume percentage of 5%-8% of th...
Embodiment 2
[0057] Microbial agent WSW of the present invention and contrast CK (do not add any passivating agent) reduce the experimental result of the cadmium content of each organ of rice plant at different growth stages of rice, see figure 1 and Table 1.
[0058] The usage method of the microbial agent of the present invention is as follows: before soil preparation, the agent is evenly spread on the whole paddy field at a rate of 100 kg / mu, the field is plowed, and the soil can be planted after plowing and raking.
[0059] Table 1 The cadmium-reducing effect of the microbial inoculum product of the present invention applied on rice
[0060]
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Abstract
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