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System and method for detecting cell elastic modulus

A technology of elastic modulus and detection system, which is used in the application of stable tension/pressure to test the strength of materials, measuring devices, strength characteristics, etc., which can solve the problems of difficult to achieve continuous research, cell contamination, cell damage

Active Publication Date: 2014-12-17
SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, using an atomic force microscope to detect the elastic modulus of cells requires taking the cells out of the culture dish and using probes to directly contact the cells to detect, which will damage the cells or cause the cells to be infected with bacteria. , the detected cells can no longer be cultured, making it difficult to achieve continuous research on the same cell in different states

Method used

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  • System and method for detecting cell elastic modulus
  • System and method for detecting cell elastic modulus
  • System and method for detecting cell elastic modulus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0098] (1) Add microspheres with a particle size of 0.5 micron and made of polystyrene into the carbon dioxide incubator, then start the lighting source, and move the stage so that the microspheres in the carbon dioxide incubator are on the objective lens of the inverted microscope imaging.

[0099] (2) Start the laser light source, and rotate at least one of the first gimbal mirror and the second gimbal mirror, so that the laser potential well traps the microspheres.

[0100] (3) Move the stage at a constant speed so that the chronic myeloid leukemia cells move towards the direction of the microspheres to deform the chronic myelogenous leukemia cells, then stop moving the stage and keep the stage in place for 10 seconds; Then move the stage at a constant speed again to separate the chronic myelogenous leukemia cells from the microspheres. At the same time, use the 3D photosensitive detection device to detect the real-time position of the microsphere, and transmit it to the c...

Embodiment 2

[0103] (1) Add microspheres with a particle size of 0.5 micron and made of polystyrene into the carbon dioxide incubator, then start the lighting source, and move the stage so that the microspheres in the carbon dioxide incubator are on the objective lens of the inverted microscope imaging.

[0104] (2) Start the laser light source, and rotate at least one of the first gimbal mirror and the second gimbal mirror, so that the laser potential well traps the microspheres.

[0105] (3) Move the stage at a constant speed so that the acute myeloid leukemia cells move towards the direction of the microspheres to deform the acute myeloid leukemia cells, then stop moving the stage and keep the stage in place for 10 seconds; Then move the stage at a constant speed again to separate the acute myeloid leukemia cells from the microspheres. At the same time, use the 3D photosensitive detection device to detect the real-time position of the microsphere, and transmit it to the computer, and t...

Embodiment 3

[0108] (1) Add microspheres with a particle size of 0.5 micron and made of polystyrene into the carbon dioxide incubator, then move the stage to start the lighting source, and make the microspheres in the carbon dioxide incubator image on the objective lens of the inverted microscope .

[0109] (2) Start the laser light source, and rotate at least one of the first gimbal mirror and the second gimbal mirror, so that the laser potential well traps the microspheres.

[0110] (3) Move the stage at a constant speed so that the granulocytes move towards the direction of the microspheres to deform the granulocytes, then stop moving the stage and keep the stage in place for 10 seconds. Then move the stage at a constant speed again to separate the granulocytes from the microspheres. At the same time, use the 3D photosensitive detection device to detect the real-time position of the microsphere, and transmit it to the computer, and the computer will perform image analysis and data proc...

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Abstract

The invention discloses a system for detecting a cell elastic modulus. The system comprises a laser source, a beam expander, a reflective mirror, a first polarizing beam splitter, a first articulated mirror, a second polarizing beam splitter, a second articulated mirror, a first color selective mirror, an objective table, a carbon dioxide culture case, an inverted microscope, an illumination light source, a second color selective mirror, a photosensitive detection device and a computer. The laser source emits a laser beam, the objective table is mobile, cells and microballoons are arranged in the carbon dioxide culture case, the carbon dioxide culture case is fixed to the objective table, the carbon dioxide culture case and the objective table are transparent, the inverted microscope is provided with an eyepiece and an objective, the photosensitive detection device is in a communication connection relationship with the computer, and the computer is used for image analysis and data processing. The system for detecting a cell elastic modulus can carry out the whole detection process under the cell culture conditions, does not pollute and damage cells and can realize follow-up culture after detection. The invention also provides a method for detecting a cell elastic modulus.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a detection system for the elastic modulus of cells and a detection method for the elastic modulus of cells. Background technique [0002] The elastic modulus of cells is closely related to cell death, migration, and differentiation. Therefore, it is often necessary to study the elastic modulus of cells in the research of cells. When using traditional detection methods to detect the elastic modulus of cells, the detection equipment needs to be come into direct contact with cells. For example, using an atomic force microscope to detect the elastic modulus of cells requires taking the cells out of the culture dish and using probes to directly contact the cells to detect, which will damage the cells or cause the cells to be infected with bacteria. , the detected cells can no longer be cultured, which makes it difficult to achieve continuous research on the same cell in diff...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N3/08G01N3/06
Inventor 唐斌颜庆云周卓龙葛永梅
Owner SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA