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Cell elastic modulus detection system and cell elastic modulus detection method

A technology of elastic modulus and detection system, which is applied in the direction of applying stable tension/pressure to test the strength of materials, measuring devices, strength characteristics, etc., and can solve problems such as cell damage, difficulty in achieving sustainable research, and cell contamination

Active Publication Date: 2016-08-24
SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, using an atomic force microscope to detect the elastic modulus of cells requires taking the cells out of the culture dish and using probes to directly contact the cells to detect, which will damage the cells or cause the cells to be infected with bacteria. , the detected cells can no longer be cultured, making it difficult to achieve continuous research on the same cell in different states

Method used

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  • Cell elastic modulus detection system and cell elastic modulus detection method
  • Cell elastic modulus detection system and cell elastic modulus detection method
  • Cell elastic modulus detection system and cell elastic modulus detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0098] (1) Add microspheres with a particle size of 0.5 micron and made of polystyrene into the carbon dioxide incubator, then start the lighting source, and move the stage so that the microspheres in the carbon dioxide incubator are on the objective lens of the inverted microscope imaging.

[0099] (2) Start the laser light source, and rotate at least one of the first gimbal mirror and the second gimbal mirror, so that the laser potential well traps the microspheres.

[0100] (3) Move the stage at a constant speed so that the chronic myeloid leukemia cells move towards the direction of the microspheres to deform the chronic myelogenous leukemia cells, then stop moving the stage and keep the stage in place for 10 seconds; Then move the stage at a constant speed again to separate the chronic myelogenous leukemia cells from the microspheres. At the same time, use the 3D photosensitive detection device to detect the real-time position of the microsphere, and transmit it to the c...

Embodiment 2

[0103] (1) Add microspheres with a particle size of 0.5 micron and made of polystyrene into the carbon dioxide incubator, then start the lighting source, and move the stage so that the microspheres in the carbon dioxide incubator are on the objective lens of the inverted microscope imaging.

[0104] (2) Start the laser light source, and rotate at least one of the first gimbal mirror and the second gimbal mirror, so that the laser potential well traps the microspheres.

[0105] (3) Move the stage at a constant speed so that the acute myeloid leukemia cells move towards the direction of the microspheres to deform the acute myeloid leukemia cells, then stop moving the stage and keep the stage in place for 10 seconds; Then move the stage at a constant speed again to separate the acute myeloid leukemia cells from the microspheres. At the same time, use the 3D photosensitive detection device to detect the real-time position of the microsphere, and transmit it to the computer, and t...

Embodiment 3

[0108] (1) Add microspheres with a particle size of 0.5 micron and made of polystyrene into the carbon dioxide incubator, then move the stage to start the lighting source, and make the microspheres in the carbon dioxide incubator image on the objective lens of the inverted microscope .

[0109] (2) Start the laser light source, and rotate at least one of the first gimbal mirror and the second gimbal mirror, so that the laser potential well traps the microspheres.

[0110] (3) Move the stage at a constant speed so that the granulocytes move towards the direction of the microspheres to deform the granulocytes, then stop moving the stage and keep the stage in place for 10 seconds. Then move the stage at a constant speed again to separate the granulocytes from the microspheres. At the same time, use the 3D photosensitive detection device to detect the real-time position of the microsphere, and transmit it to the computer, and the computer will perform image analysis and data proc...

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Abstract

A detection system for the elastic modulus of cells, comprising a laser light source, a beam expander, a mirror, a first polarizing beam splitter, a first universal mirror, a second polarizing beam splitter, a second universal mirror, a first dichroic Mirror, stage, carbon dioxide incubator, inverted microscope, lighting source, second dichroic mirror, photosensitive detection device and computer, laser light source emits laser beam, stage is movable, and cells and microspheres are installed in the carbon dioxide incubator, And the carbon dioxide incubator is fixed on the stage, both the carbon dioxide incubator and the stage can transmit light, the inverted microscope has an eyepiece and an objective lens, the photosensitive detection device is connected with a computer for image analysis and data processing. The whole detection process of the above-mentioned cell elastic modulus detection system is carried out under the condition of cell culture, which will not cause pollution and damage to the cells, and can continue to be cultured after detection. In addition, a method for detecting the elastic modulus of cells is also provided.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a detection system for the elastic modulus of cells and a detection method for the elastic modulus of cells. Background technique [0002] The elastic modulus of cells is closely related to cell death, migration, and differentiation. Therefore, it is often necessary to study the elastic modulus of cells in the research of cells. When using traditional detection methods to detect the elastic modulus of cells, the detection equipment needs to be come into direct contact with cells. For example, using an atomic force microscope to detect the elastic modulus of cells requires taking the cells out of the culture dish and using probes to directly contact the cells to detect, which will damage the cells or cause the cells to be infected with bacteria. , the detected cells can no longer be cultured, which makes it difficult to achieve continuous research on the same cell in diff...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N3/08G01N3/06
Inventor 唐斌颜庆云周卓龙葛永梅
Owner SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA