Method for killing Listeria monocytogenes in low-temperature stress enhanced pulsed electric field
A monoproliferative Listeria monocytogenes, pulsed electric field technology, applied in the application, preservation of milk, preservation of eggs by radiation/electrical treatment, etc. Energy consumption, the effect of reducing requirements
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Embodiment 1
[0033] Fresh lychee juice is divided into two parts, one is not inoculated with Listeria monocytogenes, and the other is inoculated with Listeria monocytogenes to make the number reach 1.0×10 7cfu / cm; two fresh lychee juices were stored at programmed cooling: first at 15°C for 1 h, and then at 5°C for 1 h. Then, the lychee juice was heated to 40°C, and the whole heating process was completed within 1 min; the pulsed electric field treatment was performed immediately, and the electrical parameters of the pulsed electric field treatment were: electric field intensity 30kV / cm, number of pulses 200, frequency 1000Hz, pulse width 1000Hz 2μs; the lychee juice treated by the pulsed electric field is pumped into the heat exchanger by peristalsis, and the lychee juice that has been cooled and stored by the program but not treated by the pulsed electric field is also pumped into the heat exchanger, so that the The lychee juice treated by the pulsed electric field was heated to 40 °C; th...
Embodiment 2
[0038] The fresh milk is divided into two parts, one is not inoculated with L. monocytogenes, and the other is inoculated with L. monocytogenes so that the number reaches 1.0 × 10 7 cfu / cm; program cooling storage for two milks: first at 15°C for 1 h, then at 5°C for 2 h. Then, heat the milk to 35℃ with a heat exchanger, the whole heat exchange process is completed within 1min, and the pulse electric field treatment is performed immediately. The electrical parameters of the pulse electric field treatment are: electric field intensity 25kV / cm, number of pulses 100, frequency 1000Hz , the pulse width is 40μs. The milk treated by the pulsed electric field is pumped into the heat exchanger by peristalsis, and the milk that has been cooled and stored by the program but not treated by the pulsed electric field is also pumped into the heat exchanger, so that the milk that has been cooled and stored by the program but not treated by the pulsed electric field is also pumped into the he...
Embodiment 3
[0042] The fresh egg liquid is divided into two parts, one part is not inoculated with Listeria monocytogenes, and the other part is inoculated with Listeria monocytogenes to make the number reach 1.0×10 7 cfu / cm; two egg liquids were stored under programmed cooling; first, they were stored at 15°C for 2 hours, and then at 5°C for 2 hours. Then, the milk was heated to 38°C with a heat exchanger, the whole heat exchange process was completed within 1.5min, and the pulse electric field treatment was performed immediately. The electrical parameters of the pulse electric field treatment were: electric field intensity 15kV / cm, number of pulses 50, frequency of 1000Hz, the pulse width is 100μs. The egg liquid treated by the pulse electric field is pumped into the heat exchanger by peristaltic pump for heat exchange with the egg liquid before treatment, and then it is filled under aseptic conditions immediately.
[0043] At the same time, the plate count method was used to determine...
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