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Uses of human defb1

A technology for sperm and asthenozoospermia, which is applied in the application field of human DEFB1 to achieve the effect of promoting sperm motility and sterilization, sperm quality and egg penetration ability

Active Publication Date: 2017-11-07
THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although, the rat isoform Binb of human DEFB1 has been extensively studied and shown to be involved in intrasperm Ca 2+ Mobilization agent activates sperm motility However, there are few research reports on human DEFB1 regulating sperm function and regulating male fertility

Method used

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  • Uses of human defb1
  • Uses of human defb1
  • Uses of human defb1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0046] The present invention will be further described in detail below through specific embodiments in conjunction with the accompanying drawings.

[0047] 1. Expression and distribution of DEFB1 in sperm

[0048]The present invention respectively detects the expression and functional mechanism of DEFB1 on sperm through western blot, immunofluorescence staining, CASA detection, number of bacterial clones, sperm zona pellucida binding experiment and zona pellucida oocyte experiment, and finds that asthenospermia The level of DEFB1 in the sperm of patients with semen leukocytosis was significantly decreased, and the motility and bactericidal ability of sperm were down-regulated. The use of antibodies to interfere with the function of DEFB1 in normal sperm will down-regulate the motility and antibacterial activity of normal sperm; while the use of recombinant DEFB1 protein can significantly restore the expression of DEFB1 on sperm from asthenozoospermia and seminal leukocytosis, ...

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Abstract

The invention discloses an application of human DEFB1. The human DEFB1 can be applied in preparing a preparation for diagnosing and treating male infertility. The experiment shows that the level of DEFB1 in sperms of a patient with asthenospermia and semen leukocytosis is remarkably decreased, and the vitality and the bactericidal ability of the sperms are weakened; the recombinant DEFB1 protein which is adopted can be used for remarkably restoring the expression of the DEFB1 on the sperms subjected to the asthenospermia and the semen leukocytosis, so that the bactericidal ability, the quality and the spawn penetrating ability of the sperms are obviously improved; and the DEFB1 is combined with a transporter / receptor CCR6 on each sperm, so that the DEFB1 has dual functions of promoting the sperm vitality and performing the bactericidal action in male fertility. Thus, based on the characteristics, the human DEFB1 can be prepared into the corresponding preparations for treating and diagnosing the male infertility.

Description

technical field [0001] This application relates to a use of human DEFB1. Background technique [0002] Worldwide, infertility affects ~10-15% of couples, with male infertility accounting for approximately 50%. There are many reasons for male infertility, among which sperm dysfunction is the main and most common reason. Low sperm motility, that is, asthenospermia, is a common cause of male infertility, accounting for about 18% of low fertility or infertility, and is considered to be one of the most important factors affecting the conception of normal sperm and eggs. Male reproductive tract infection is another cause of infertility. The common pathological type is orchitis or epididymitis, accounting for about 11% of male infertility. Among them, the number of white blood cells in semen increases, also known as seminal leukocytosis, which affects about 5-10% of men and is a diagnostic index for male reproductive tract infection. Interestingly, the semen of patients with ast...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/17C07K14/47A61P15/08
Inventor 刁瑞英牟丽莎黄卫人蔡学泳蔡志明
Owner THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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