Method for producing feather cockscomb thumb flower

A technology of Qingzhi Thumb and Qingzhi, which is applied in the field of producing Qingzhi Thumb, can solve the problems of production, failure, and inability to maintain the flowering period for a long time, and achieve the effects of prolonging the flowering time, miniature and cute shape, and strong adaptability

Active Publication Date: 2015-01-07
海南渊圆生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Before the present invention was made, the problems existing in the present test-tube flower technology were that it was controlled by the season, the flowering time was uncontrollable, it could not be produced by rapid

Method used

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Examples

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Effect test

Embodiment

[0050] In this example, wild Pleurotus chinensis seeds were collected directly, and the plump seeds were screened out through a sieve. The cleaned and sterilized seeds were inoculated on the primary culture medium, and cultured at 25±2°C and 2000±500lux light conditions for 8 hours a day.

[0051] Cut off the hypocotyls of the 20-day-old C. japonica seedlings from about 1 cm below the cotyledons, discard the hypocotyls and root systems, and transfer the cotyledons and growth points to the flower bud induction medium. In this example, the flower bud induction medium is: MS basic medium with 20g of sucrose, 6g of carrageenan, 0.3mg of abscisic acid, 1L of water, and adjust the pH to 5.8; Cultivate under conditions, light 8 hours a day, and cultivate for 20 days.

[0052] Prune the root system of the chrysanthemum seedlings induced by flower buds, and then transfer them to the flowering medium. In this example, the flowering medium is preferably: improved MS medium, N content re...

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Abstract

The invention relates to a method for producing a feather cockscomb thumb flower. The method comprises the steps of selecting a feather cockscomb seed as an explant, inoculating the explant on the surface of a primary culture medium; cutting off a hypocotyl of a feather cockscomb seedling; discarding the hypocotyl and a root system; grafting a cotyledon to a flower bud induction medium; after culturing the cotyledon on the flower bud induction medium, grafting the cultured cotyledon to a florescence culture medium; cutting off yellow leaves and a root system at the base part of the feather cockscomb seedling on which the inflorescence appears, and grafting the feather cockscomb seedling to a freshness-retaining culture medium for culturing; cutting off the root part and leaves of the main stem of the base part of the feather cockscomb seedling, and inserting the feather cockscomb seedling to a breeding culture medium to obtain a sub-cultured seedling; selecting a stem section with a flower bud at the top end and vertically inserting the stem section into a rooting culture medium until roots grow so as to finish the culture of the thumb flower. The method for producing the feather cockscomb thumb flower overcomes the defects that the flowering time is uncontrollable, rapid breeding ways such as tissue culture cannot be adopted for production, and the flower period cannot be kept for a long time. According to the method for producing the feather cockscomb thumb flower, as the tissue culture way is used for breeding, the flowering condition can be controlled, the flowering time of the plant can be prolonged efficiently, and the thumb flowers can be produced rapidly and industrially.

Description

technical field [0001] The invention relates to a method for producing Thumbhus japonica, in particular to a method for producing Thumbhus chinensis. Background technique [0002] Thumb flower, also known as test-tube flower, is a miniature ornamental flower produced by plant tissue culture rapid propagation technology in a closed glass vessel by changing the medium composition and culture conditions and artificially controlling the growth stage of the plant. [0003] The test tube flower is a popular mini portable flower in the market at present. It is named for its small plant and grows in a delicate small test tube or glass bottle. It is a general term for a series of miniature plants that are grown in a thumb-sized transparent test tube or artistic glass bottle under relatively or absolutely sterile conditions by using plant tissue culture technology. [0004] At present, the technology of commercially available test-tube flowers is still immature, and can be roughly di...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈晓晖王台虎
Owner 海南渊圆生物技术有限公司
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