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Ovarian cancer stem cell vaccine and its preparation method and application

A technology for ovarian cancer cells and ovarian cancer, applied in the direction of tumor/cancer cells, animal cells, vertebrate cells, etc., can solve the problems of low curative effect and lack of ovarian cancer stem cell vaccines, etc., to enhance curative effect, enhance immune function, and strengthen the body The effect of defense

Active Publication Date: 2016-10-05
广州复大医疗有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, radiotherapy and chemotherapy have little effect on the small number of stem cell-like cells present in the tumor, and the proliferation of ovarian cancer stem cells remaining after treatment is enough to promote one of ovarian cancer recurrence and metastasis.
Still lack effective ovarian cancer stem cell vaccine at present, those skilled in the art urgently need to make a breakthrough in this respect

Method used

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  • Ovarian cancer stem cell vaccine and its preparation method and application
  • Ovarian cancer stem cell vaccine and its preparation method and application
  • Ovarian cancer stem cell vaccine and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1: Preparation of Ovarian Cancer Stem Cell Vaccine

[0042] In this example, ovarian cancer stem cell vaccines were prepared using ovarian cancer cells stored in liquid nitrogen as raw materials.

[0043] 1. Put the ovarian cancer cells SK-OV-3 stored in liquid nitrogen or -80°C in a 37°C water bath to dissolve quickly;

[0044] 2. Mix the thawed tumor cell suspension with 8.0ml medium (RPMI1640+10% FBS), and centrifuge at 1500 rpm for 8 minutes;

[0045] 3. Discard the supernatant, then absorb 8.0ml culture medium to mix the cell pellet, then centrifuge at 1500 rpm for 8 minutes, discard the supernatant, mix the cell pellet with 1.0ml culture medium, and set aside;

[0046] 4. Take another 75cm 2 Square bottle, add 14.0ml of culture medium, add the suspension containing tumor cells (1.0ml) prepared above into this square bottle, at 37°C, 5% CO 2 cultured in an incubator;

[0047] 5. Tumor cells grow on the wall. After 3-4 days, when the tumor cells grow to ...

Embodiment 2

[0064] Example 2: Curative Effect Observation After Injection of Ovarian Cancer Stem Cell Vaccine

[0065] Apply the ovarian cancer stem cell vaccine prepared in Example 1 to the treatment of ovarian cancer patients. The detailed experimental process is as follows: 30 ovarian cancer patients were randomly selected and divided into two groups, 15 of which received the vaccine injection treatment prepared in Example 1. , as the treatment group; the other 15 people who did not receive vaccine treatment were used as the control group. Before injecting patients, 5ml of blood was drawn from each patient, and the number of peripheral blood lymphocytes (T BNK detection kit) and lymphocyte function (6colour T BNK With Trucount detection kit) were detected, and the report sheet was obtained through flow cytometry detection. For patients in the treatment group, inject 500ul of the vaccine dose subcutaneously into the ovarian cancer patients, and inject it again after a week interval, tha...

Embodiment 3

[0070] Example 3: Humoral immune response induced by the tumor stem cell vaccine prepared in Example 1

[0071] 30 routine patients in the embodiment 1 are equally divided into two groups (no vaccine injection group-control group and vaccination group-experimental group), carry out according to the following method respectively:

[0072] Collect 5ml of the whole blood of the patient on the 14th day, and separate the plasma with the lymphocyte separation medium. The details are as follows: Take 5ml of fresh anticoagulant blood, mix it with normal saline for injection at a ratio of 1:1, and carefully add it to 2ml of the lymphocyte separation medium , centrifuge at 400g (horizontal rotor with a radius of 15cm) for 20 minutes. At this time, the centrifuge tube is divided into four layers, and the uppermost layer is plasma. The IgG content in plasma was detected by ELISA.

[0073] The result is as image 3 As shown, compared with the control group, P image 3 It can be seen that ...

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Abstract

The invention discloses a tumor stem cell vaccine and its preparation and application. The ovarian cancer stem cell vaccine of the present invention comprises: inactivated ovarian cancer stem cells, mannan peptide and fat emulsion mixed in a volume ratio of 3:1:1. The preparation method of the ovarian cancer stem cell vaccine of the present invention includes the following steps: obtaining ovarian cancer stem cells; inactivating the ovarian cancer stem cells; Polyglycopeptide and fat emulsion are mixed to obtain the ovarian cancer stem cell vaccine. The present invention provides the use of the ovarian cancer stem cell vaccine obtained according to the preparation method of the present invention for preparing a medicine for treating ovarian cancer. The invention provides a new approach for the treatment of ovarian cancer and the preparation of vaccines, has positive therapeutic effect on ovarian cancer patients, can enhance the immune function of the patients, and has a positive effect on prolonging the survival time of the patients.

Description

technical field [0001] The invention relates to biomedical engineering technology, and relates to a tumor stem cell vaccine and its preparation and application. Background technique [0002] Malignant tumor is a systemic chronic disease. In patients with middle and advanced stages, there are not only obvious mass tumors in the organs during imaging examinations, but also a large number of invisible circulating tumor cells and cancer stem cells (Cancer stem cells, CSCs) in the peripheral blood, ready to settle in the body at any time Susceptible sites develop into new metastases, which is why the tumor cannot be cured. Traditional tumor treatment includes 4 main therapies, namely surgery, chemotherapy, radiotherapy and immunotherapy. Usually, after 3-5 years of treatment with the first three therapies, the tumor will metastasize to peripheral organs or distant organs. With the in-depth research on CSC, it has been clear that the reason for recurrence and metastasis is not ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/00A61K39/39C12N5/095A61P15/08A61P35/00
Inventor 陈继冰林茂左建生刘建国徐克成牛立志
Owner 广州复大医疗有限公司
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