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Acinetobacter strain for low-temperature denitriding and application thereof

A low-temperature, denitrification technology, used in bacteria, water/sludge/sewage treatment, biochemical equipment and methods, etc., to achieve a wide range of growth conditions

Active Publication Date: 2015-01-07
INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at present, there are many studies on the isolation, identification, cultivation, and cloning of related functional genes for denitrification strains at home and abroad, but there is no report on the safety of denitrification strains, and the safety of low-temperature degradation strains is a key factor in the application of strains. important factors before

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  • Acinetobacter strain for low-temperature denitriding and application thereof
  • Acinetobacter strain for low-temperature denitriding and application thereof
  • Acinetobacter strain for low-temperature denitriding and application thereof

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specific Embodiment approach 1

[0019] Specific embodiment 1: The low-temperature denitrifying Acinetobacter in this embodiment is Acinetobacter sp. bd-01, which is preserved in the General Microbiology Center (CGMCC) of the China Microorganism Culture Collection and Management Committee, and the preservation address is: Chaoyang, Beijing No. 3, No. 1 Courtyard, Beichen West Road, District, the preservation date is September 1, 2014, and the preservation number is CGMCC No.9627.

[0020] In this embodiment, Acinetobacter bd-01 was isolated and screened from Songhua River water in Heilongjiang Province in March. The separation method is carried out according to the following steps: take 100 mL of river water and put it into a 500 mL conical flask, add 50 mg of ammonium sulfate, 300 mg of sodium acetate and 5 mL of trace element solution, enrich and culture at 6 °C and 160 r / min for 5 days, and take 1 mL of enriched culture solution. Add 3 parallel samples into a conical flask containing 50 mL of basal medium,...

specific Embodiment approach 2

[0024] Specific embodiment 2: This embodiment of Acinetobacter sp. bd-01 is used for denitrification of domestic sewage, agricultural waste and industrial wastewater in low temperature environment, and the low temperature is 6°C.

[0025]This embodiment of Acinetobacter bd-01 has high-efficiency denitrification ability at low temperature (6°C), can degrade ammonia nitrogen in a wide range of concentrations (0.50mg / L-951.09mg / L), and can be applied to domestic sewage in low-temperature environments. The denitrification of water bodies such as agricultural waste and industrial wastewater is of great significance to the treatment and restoration of water bodies in low temperature environments.

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Abstract

The invention discloses an acinetobacter strain for low-temperature denitriding and an application thereof and relates to an acinetobacter strain and an application thereof. The acinetobacter strain is preserved in China General Microbiological Culture Collection Center on September 1, 2014 and has the preservation number of CGMCC No. 9627. Since the acinetobacter strain bd-01 disclosed by the invention is a heterotrophic nitrification bacterium and can have a heterotrophic nitrification reaction at a low temperature of 6 DEG C so as to convert ammoniacal nitrogen into nitric nitrogen, nitrite nitrogen and intracellular substances. By adopting the acinetobacter strain, the total ammonia can be efficiently and thoroughly removed and the degradation rate of nitrogen is100% at the low temperature of 6 DEG C within 48 hours. The acinetobacter strain for low-temperature denitriding can be used for denitriding domestic sewage, agricultural wastes and industrial wastewater in areas with a low-emperature environment.

Description

technical field [0001] The present invention relates to a strain of Acinetobacter and its application. Background technique [0002] Nitrogen content is an important indicator of wastewater quality. In recent years, with the rapid development of my country's economy, the discharge of urban domestic sewage has become larger and larger, and the nitrogen content has become higher and higher. The high concentration of nitrogen will cause oxygen deficit in the receiving water body, breed harmful aquatic organisms, and affect agricultural production and aquatic products. Breeding brings great losses, which has attracted more and more people's attention. There are biological methods and physicochemical methods for denitrification of wastewater. However, due to the high cost of the physicochemical method and the possibility of secondary pollution, its promotion is limited. Biological denitrification has the characteristics of economy, effectiveness, easy operation and no secondary...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01
CPCC02F3/34C12N1/205C12R2001/01
Inventor 姜威张淑梅李晶陈静宇孟利强胡基华曹旭刘宇帅
Owner INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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