Preparation method of polypeptide oral solution
A technology of peptide oral liquid and hydrolyzate, which is applied in the field of preparation of peptide oral liquid, can solve problems such as poor taste, stickiness, and prone to burning, and achieve the effect of promoting calcium absorption, obvious effect, and good taste
Inactive Publication Date: 2015-01-28
西安莹朴生物科技股份有限公司
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AI Technical Summary
Problems solved by technology
This kind of product often has some problems in the process of eating, such as the lump donkey-hide gelatin boiled for a long time, which is prone to burnt phenomenon; donkey-hide gelatin oral liquid also has a certain burnt smell, and is sticky and poor in taste. These problems are seriously restricted. Further development of donkey-hide gelatin products
Method used
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Experimental program
Comparison scheme
Effect test
Embodiment 1
[0011] Scrape off the fat and hair attached to the donkey skin, cut it into small pieces after cleaning, grind it with a meat grinder, weigh 900g of the donkey skin, grind it twice with a colloid mill (material: water = 1:5), and mix with Boil carrots, white vinegar, and white wine for 5 minutes at the same time, add distilled water to 10L (concentration: 9%), heat to 45°C on a constant temperature water bath and keep the temperature constant. Prepare 1mol / L sodium hydroxide and 1mol / L hydrochloric acid solution, adjust the pH to always be pH7.0 with a pH automatic regulator, add A.S1398 neutral protease, the dosage is 7000U / g, hydrolyze for 1h, boil for 5min to extinguish Enzyme activity, after cooling, centrifuge at 8000rpm for 15min, and take the supernatant. First adjust the pH of the hydrolyzate to 4.5 with citric acid, filter after standing for 30 minutes, and then adjust the pH to 6.0 with sodium bicarbonate, that is, use the isoelectric point sedimentation method to re...
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The invention relates to a preparation method of a polypeptide oral solution, belonging to the field of a preparation method of a nutritional food, especially a nutritional oral solution. The preparation method of the polypeptide oral solution comprises the following steps: scraping off fat and hairs attached on a donkey skin; washing and cutting the donkey skin into small blocks; mincing by a mincing machine; finely milling for two times by a colloid mill (at a ratio of materials to water of 1 to 5; adding distilled water and putting into a constant-temperature water bath device to be heated and keeping a constant temperature; adjusting the pH to be the optimal pH of protease; adding the protease and hydrolyzing; after hydrolyzing, boiling for 5 minutes for enzyme deactivation; after cooling, centrifuging and separating for 15 minutes at the speed of 8000rpm; taking liquid supernatant and adjusting the pH of hydrolyzed liquid to 4.5 by citric acid; standing for 30 minutes and filtering; then adjusting the pH to 6.0 by using sodium bicarbonate; and adding beta-cyclodextrine, citric acid liquid and protein sugar into the hydrolyzed liquid to remove a bitter taste and odor to obtain the polypeptide oral solution. According to the polypeptide oral solution, the content of low-molecular peptides is more than or equal to 1.3% and the product is clarified and transparent, has no sediment and has a good taste. An animal test is carried out to observe the effects of enriching blood, strengthening bones and promoting calcium absorption of the donkey skin polypeptide oral solution, and the test result shows that the polypeptide oral solution has no remarkable difference when being compared with donkey-hide gelatin (P is more than 0.05), and has obvious effects on peripheral hemogram of white rats suffering from anemia.
Description
technical field [0001] The invention relates to a preparation method of polypeptide oral liquid, which belongs to the field of nutritional products, especially nutritional oral liquid and its preparation method. Background technique [0002] Modern biological experiments have proved that polypeptides have more advantages compared with proteins and amino acids: polypeptides have the best absorption rate in the intestinal tract, and some low-level peptides such as dipeptides and tripeptides can absorb faster than amino acids of the same composition; polypeptides have low Antigen, promoting lipid metabolism, lowering cholesterol, promoting mineral absorption, lowering blood pressure, anti-oxidation and other physiological functions; some polypeptides have the effect of strengthening the body's immunity and promoting human metabolism; polypeptides have good acid, heat stability, water solubility , and the viscosity changes slowly with concentration. At present, the research on ...
Claims
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IPC IPC(8): A23L1/29A23L1/305A23L33/18
CPCA23V2002/00
Inventor 李勇刘文斌
Owner 西安莹朴生物科技股份有限公司