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Detection kit and detection method for auxiliary diagnosis of Alzheimer's disease

A technology for Alzheimer's disease and detection kits, applied in the direction of microbial measurement/testing, biochemical equipment and methods, etc., can solve the problems of no public detection kits, etc., and achieve the effect of strong pertinence and high detection efficiency

Inactive Publication Date: 2016-04-06
NINGBO UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

[0004] At present, there is no published research report on the detection kit used to detect the degree of methylation of the promoter region of the G protein-coupled receptor gene associated with Alzheimer's disease at home and abroad.

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  • Detection kit and detection method for auxiliary diagnosis of Alzheimer's disease
  • Detection kit and detection method for auxiliary diagnosis of Alzheimer's disease
  • Detection kit and detection method for auxiliary diagnosis of Alzheimer's disease

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Embodiment Construction

[0027] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0028] A detection kit for auxiliary diagnosis of Alzheimer's disease, the detection kit includes a pair of G protein-coupled receptor gene promoter region methylation-specific amplification primers and a methylation-specific sequencing primer, the The above-mentioned pair of G protein-coupled receptor gene promoter region methylation-specific amplification primers include methylation-specific upstream primers and methylation-specific downstream primers:

[0029] The methylation-specific upstream primers include the following nucleotide sequences:

[0030] 5'-GGGGATTTAGAGAGGTTGTAAAG-3';

[0031] The methylation-specific downstream primers include the following nucleotide sequences:

[0032] 5'-Biotin-CCAACCTATAAACCCAACTAACTACTCTAC-3';

[0033] The methylation-specific sequencing primers include the following nucleotide sequences:

[0034] ...

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Abstract

The invention discloses a detection kit for auxiliary diagnosis of Alzheimer's disease, which comprises a pair of G protein coupled receptor gene promoter region methylation-specific amplification primers and a methylation-specific sequencing primer, wherein the pair of G protein coupled receptor gene promoter region methylation-specific amplification primers comprises a methylation-specific forward primer and a methylation-specific reverse primer. The Alzheimer's disease-related G protein coupled receptor gene promoter region methylation degree is detected to implement auxiliary diagnosis of Alzheimer's disease; and the kit and method are simple and convenient, have the advantages of high detection efficiency, high pertinence, high accuracy, high reliability, high flexibility, high speed and cost saving, and are beneficial to early detection and timely treatment of Alzheimer's disease.

Description

technical field [0001] The present invention relates to the technical field of auxiliary diagnosis of Alzheimer's disease, in particular to a detection kit for auxiliary diagnosis of Alzheimer's disease and a detection method thereof, especially to a detection kit that can be used to detect Alzheimer's disease-related A kit for detecting the degree of methylation in the promoter region of the G protein-coupled receptor 50 gene and a detection method thereof. Background technique [0002] Alzheimer's disease (Alzheimer's disease, AD) is a systemic neurodegenerative disease, and its clinical manifestations are low-level cognitive function, and its main features include neurofibrillary tangles in brain cells and senile plaques outside cells. Patients with Alzheimer's disease require life-long care, thus placing a great financial burden on society and families. Alzheimer's disease is a complex disease caused by the joint action of genetic factors and environmental factors. Find...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6883C12Q2600/154
Inventor 段世伟季慧慧王钦文刘桂利
Owner NINGBO UNIV