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Bacterial strains for degrading 5-ring and 6-ring polycyclic aromatic hydrocarbons, their acquisition method and application

A technology for polycyclic aromatic hydrocarbons and strains, which is applied in the field of biological treatment of environmental pollutants, can solve problems such as unsatisfactory degradation effect of polycyclic aromatic hydrocarbons, and achieve the effect of repairing polluted sites.

Inactive Publication Date: 2017-09-15
HEBEI AGRICULTURAL UNIV.
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the bacteria that degrade aromatic hydrocarbons in the past are aimed at low molecular weight PAHs, and the degradation effect on 5-ring and 6-ring PAHs is not ideal, especially for the screening of degrading bacteria that use PAHs-contaminated soil in coal mining areas as a medium. There are reports

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  • Bacterial strains for degrading 5-ring and 6-ring polycyclic aromatic hydrocarbons, their acquisition method and application
  • Bacterial strains for degrading 5-ring and 6-ring polycyclic aromatic hydrocarbons, their acquisition method and application
  • Bacterial strains for degrading 5-ring and 6-ring polycyclic aromatic hydrocarbons, their acquisition method and application

Examples

Experimental program
Comparison scheme
Effect test

example (1

[0054] Example (1): Degradation of ZH-L8 to benzopyrene monomer

[0055] Inoculate the ZH-H2 single colony preserved after the PAH monomer acclimatization in the above step (4) into LB medium and activate for 48 hours to obtain the enriched bacterial liquid.

[0056] Inoculate 10% enriched bacteria solution into 20mL sterilized aqueous phase inorganic salt liquid medium containing 10mg / benzopyrene as the experimental group, and another control group without enriched bacteria solution, in 150rmp, 30°C shaker Shake culture in the dark for 7 days.

[0057] After the incubation time is over, take 25ml of chromatographically pure n-hexane solution and add it to the Erlenmeyer flasks of the experimental group and the control group, ultrasonically extract for 5 minutes, so that all the PAHs sticking to the wall are soaked into the n-hexane solution, and then the Erlenmeyer flasks are placed in the n-hexane solution. In a 250rmp oscillator, after oscillating for 40 minutes and ultras...

example (2

[0059] Example (2): Degradation of indenopyrene monomer by bacterial strain ZH-L8

[0060] Inoculate 10% enriched bacteria solution into 20mL sterilized aqueous phase inorganic salt medium containing 10mg / L indenopyrene as the experimental group, and another control group without enriched bacteria solution, in 150rmp, 30°C shaker Shake and cultivate in the dark for 7 days, the method for extracting PAHs is the same as example (1), measure the residual rate of polycyclic aromatic hydrocarbons in the solution of the experimental group and the control group respectively, then apply the residual rate of the experimental group and the control group, according to the degradation formula, Calculate the degradation efficiency.

[0061] The experiment was repeated three times, and the results were averaged. The degradation rate of indenopyrene is 42%.

example (3

[0062] Example (3): Degradation of dibenzoanthracene monomer by bacterial strain ZH-L8

[0063] Inoculate 10% enriched bacteria solution into 20mL sterilized aqueous phase inorganic salt medium containing 10mg / L dibenzanthracene as the experimental group, and another control group without enriched bacteria solution, shake at 150rmp, 30°C Shake culture in medium dark place for 7 days, the method for extracting PAHs is the same as example (1), measure the residual rate of polycyclic aromatic hydrocarbons in the solution of experimental group and control group respectively, apply the residual rate of experimental group and control group then, according to the degradation formula , to calculate the degradation efficiency.

[0064] The experiment was repeated three times, and the results were averaged. The degradation rate of dibenzanthracene was 72%.

[0065] The embodiment of the present invention also provides the application of the above-mentioned bacterial strain ZH-L8 to th...

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Abstract

The embodiment of the present invention provides a bacterial strain for degrading 5-ring and 6-ring polycyclic aromatic hydrocarbons, and its acquisition method and application, which relate to the technical field of biological treatment of environmental pollutants and can efficiently degrade 5-ring and 6-ring polycyclic aromatic hydrocarbons. Described bacterial strain name is Acinetobacter baumannii (Acinetobacter baumannii) ZH-L8; Morphological characteristic is: the growth on solid culture medium is round milky-white bacterium colony, and edge is smooth; Observation is rod-shaped under the microscope; Cell is Gram-positive rod Shaped, parallel sides, blunt rounded ends; round colonies, smooth edges, close to the culture medium, white, opaque; physiological and biochemical characteristics: bacteria grow well in the range of 20‑45 °C, at pH 5.7, 6.8 Good growth; salt tolerance is 5%; methyl red positive; catalase positive; v-p negative, final pH of v-p solution <6; arginine utilization positive; nitrate utilization positive; D-glucose produces acid, Lactose acid production, sucrose acid production positive; starch hydrolysis positive, etc.

Description

technical field [0001] The invention relates to the technical field of biological treatment of environmental pollutants, in particular to a bacterial strain for degrading 5-ring and 6-ring polycyclic aromatic hydrocarbons and its acquisition method and application. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) are a class of toxic organic pollutants containing two or more benzene rings that are widely distributed and stably exist in the natural environment. Because of its "three-cause (carcinogenic, teratogenic, mutagenic) effects, it will also produce phototoxic effects, and it has the characteristics of large hidden dangers, long incubation period, wide coverage, and difficult governance in the soil, especially High-ring PAHs not only have complex chemical structures, high electron cloud density, and are difficult to be oxidized, but also have poor water solubility, strong thermal stability, high solid-water partition coefficient, and low bioavailab...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/01
CPCB09C1/10C12N1/205C12R2001/01
Inventor 杨志新赵欧亚李玉灵冯圣东石维王伟王小敏李成
Owner HEBEI AGRICULTURAL UNIV.