Microscopic imaging system capable of simultaneously obtaining appearance image and element distribution image

Abstract

The invention discloses a microscopic imaging system capable of simultaneously obtaining an appearance image and an element distribution image. The microscopic imaging system mainly comprises a laser device, a lighting source, a refracting and reflecting light path, an objective lens and a movement mechanism, wherein the laser device is used for emitting a short-pulse laser signal; the lighting source is used for emitting a visible light signal; the refracting and reflecting light path comprises a plurality of transmission reflectors, so that the light signals emitted by the laser device and the lighting source vertically diffuse downwards; the objective lens is arranged below the refracting and reflecting light path; the light signals which vertically diffuse downwards pass through the objective lens, and irradiate a to-be-detected object below the objective lens; and the movement mechanism is used for moving the light signals or moving the to-be-detected object, so that the light signals traverse all parts of the to-be-detected object. According to the microscopic imaging system, an LIBS analysis technology is organically combined with a laser scanning confocal technique; point-by-point scanning of the to-be-detected sample is achieved by adopting a spectral microscopic imaging technology; and the microscopic imaging system capable of simultaneously obtaining the appearance image and the element distribution image is achieved.

Description

technical field [0001] The invention relates to a microscopic optical imaging technology, in particular to a microscopic imaging system for simultaneously obtaining appearance images and element distribution images. Background technique [0002] High-resolution micro-optical imaging technology is a way for people to shift from the macro to the micro, to reveal the composition and regulation mechanism of life in real time at the cellular molecular level, to obtain a series of original scientific discoveries, and to realize the comprehensive cross-integration of life science, physics, chemistry and other disciplines. important means. From the earliest conventional microscopes to the current super microscopes, such as super-resolution microscopes, two-photon microscopes, and tunneling scanning microscopes, it can be said that the emergence of each new imaging technology is a milestone. With the emergence of laser technology, the application of the interaction between light and...

AI Technical Summary

Problems solved by technology

However, the traditional LIBS needs to use a relatively complex spectral analysis system, which requires a large amount of test data and a long processing time; moreover, once the sensitivity of the spectral analysis system is insufficient, it is necessary to increase the intensity of the plasma signal by increasing the intensity of the excitation light. While measuring the damage of the object, the spatial resolution is greatly reduced

Therefore, in terms of microscopic imaging, the application of LIBS has great limitations.

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