Automatic pouring device for cell culture reagents in hyperbaric chamber
A technology of cell culture and dumping device, which is applied in the direction of packaging, transportation and packaging, emptying containers, etc., to achieve the effect of reasonable structure and improved experimental efficiency
Inactive Publication Date: 2016-05-25
NANTONG UNIVERSITY
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Problems solved by technology
At present, there is no such supporting automatic device, and the addition of reagents during the experiment is done manually after decompression, so the influence of some uncertain factors on the experiment cannot be avoided
Method used
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[0016] Such as figure 1 As shown, an automatic pouring device for cell culture reagents in a hyperbaric chamber includes a hyperbaric chamber 1 , an electrical control system 2 , a transmission mechanism 3 and a liquid storage mechanism 4 .
[0017] The hatch 12 of hyperbaric chamber 1 is located at the top, which is convenient for installation of equipment in the cabin, replacement of reagents and other operations. The main function of the electrical penetrating part 13 on the side of the cabin is to connect the equipment outside the cabin with the actuator inside the cabin.
[0018] The electrical control system 2 further includes a driving power supply 21 outside the cabin, a driver 22 , a controller 23 and an actuator two-phase hybrid stepper motor 24 inside the cabin. The controller 23 selects a touch screen with good human-computer interaction, and the operator can set the operation mode and operation time by himself. The stepper motor 24 is a two-phase four-wire syste...
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The invention discloses an automatic dumping device for hyperbaric chamber cell culture reagents. A chamber door of the hyperbaric chamber is arranged at the top; the side of the chamber body is provided with an electrical penetration piece; an electric control system includes a drive mechanism positioned outside the chamber and a two-phase hybrid stepping motor located in the chamber, and is used to connect the signal lines outside the chamber to a signal terminal of the motor through the electrical penetration piece; a transmission mechanism connector connects the motor shaft of the two-phase hybrid stepping motor with a liquid storage mechanism, the connector drives the liquid storage mechanism to rotate for the same angle, so as to successfully pour reagents in one group of test tubes into the cell culture dish; a liquid storage rack is provided with a plurality of test tube place positions, two connected groups of liquid storage rack have a certain angle, and place positions of different groups of test tubes are separated. The device provided by the invention has reasonable structure, can operate normally in the hyperbaric chamber automatically dump cell culture reagents, and add a variety of reagents at the same time or add different reagents at different time, so as to improve the experimental efficiency.
Description
technical field [0001] The invention relates to an automatic dumping device for cell culture reagents in a hyperbaric chamber. Background technique [0002] In order to solve the problem of nitrogen anesthesia in humans working in the deep sea, researchers in the field of marine medicine will conduct neuron cell culture experiments in hyperbaric chambers. The medical hyperbaric chamber can simulate the pressure environment of the deep sea. The neuron cells are placed in the petri dish in the chamber. During the experiment, different exogenous reagents (agonists, inhibitors, etc.) need to be added regularly to observe and detect the pressure under high pressure. Receptor function of neuronal cells. In order to ensure that the state of neurons does not change and the accuracy of the experiment, the addition of exogenous reagents must be completed in a closed high-pressure state, and the hatch can only be decompressed and opened once the experiment is completed. At present, t...
Claims
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IPC IPC(8): B67C9/00
CPCB67C9/00
Inventor 杨奕顾海勤管图华姜正林曹海平周伯俊顾雪政杨慧季胜蓝
Owner NANTONG UNIVERSITY