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A method for simultaneous determination of 11 active ingredients in Reduning Injection
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A technology for active ingredients and injections, applied to the simultaneous determination of 11 ingredients in Reduning injection, which can solve the problems of long running time and rapid detection of unfavorable products
Active Publication Date: 2017-01-04
JIANGSU KANION PHARMA CO LTD
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Previous research established the HPLC fingerprint of Reduning Injection [3] Determination of 9 Components in Reduning Injection with One Test and Multiple Evaluation Method [4] , the above-mentioned HPLC and one test and multiple evaluation methods run for a long time, which is not conducive to the rapid detection of products in the process of industrialized large-scale production, and only detects the iridoid component of geniposide, while the iridoids The substance is found in higher levels in the prescription medicinal herbGardenia [5-6] , including hydroxygeniposide, shanzhiside, genipin gentiobioside and other ingredients, these ingredients have a certain degree of therapeutic effect on upper respiratory tract infection, and only the detection of geniposide content cannot better reflect the efficacy of the product. Quality and Efficacy
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Embodiment 1
[0069] a. Preparation of reference solution: Accurately weigh neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C, shanzhiside, and genipin Add 50% methanol to prepare appropriate amount of glycoside, genipin gentiobioside, geniposide, and deoxyloganin reference substances, the concentrations are 0.12225, 0.28953, 0.12357, 0.01606, 0.02849, 0.02323, 0.01670, 0.02468 , 0.23663, 0.40770, 0.05745 mg·ml -1 mixed reference solution.
[0071] Precisely draw 1ml of Reduning injection into a volumetric flask, dissolve it with 50% methanol and dilute to 100ml, shake well, centrifuge, and filter the supernatant through a 0.22um microporous membrane to obtain the product.
[0072] c. Determination method: adopt the UPLC-DAD method to accurately draw the reference substance solution and the test solution respectively, inject it into a liquid chromatograph, and meas...
Embodiment 2
[0077] a. Preparation of reference solution: Accurately weigh neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C, shanzhiside, and genipin glycoside, genipin gentiobioside, geniposide, deoxyloganin reference substance amount, add methanol to make the concentrations are 0.20001, 0.38333, 0.18445, 0.02661, 0.0408, 0.02865, 0.04000, 0.04845, 0.36654, 0.60076, 0.11475 mg·ml -1 mixed reference solution.
[0079] Precisely draw 1ml of Reduning injection into a volumetric flask, dissolve it with methanol and dilute to 100ml, shake well, centrifuge, and filter the supernatant through a 0.22um microporous membrane to obtain the product.
[0080] c. Determination method: adopt the UPLC-DAD method to accurately draw the reference substance solution and the test solution respectively, inject it into a liquid chromatograph, and measure it to obtain final product; ...
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Abstract
The invention discloses a method for simultaneously determining the contents of 11 kinds of effective components in a Reduning injection. The 11 kinds of effective components comprise a neochlorogenic acid, a chlorogenic acid, a cryptochlorogenic acid, an isochlorogenic acid A, an isochlorogenic acid B, an isochlorogenic acid C, shanzhiside, a geniposidic acid, genipin1-gentiobioside, geniposide and broken oxidation nux vomica glycosides. The method adopts an Agilent ZORBAX SB-C18 chromatographic column (3.0*100mm, 1.8[mu]m), a flow phase is acetonitrile (A)-0.1% phosphoric acid (B), gradient elution is adopted, a flow speed is 0.35-0.45mL . min<-1>, a detection wave length is 324-326nm and 236-238nm, and a column temperature is 25-35 DEG C. The method disclosed by the invention is accurate, quick and acute, and the quality control level of the Reduning injection is further improved.
Description
technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to a method for simultaneously determining 11 components in Reduning injection. Background technique [0002] The prescription of Reduning Injection is composed of honeysuckle, Artemisia annua, and gardenia, which has the effects of clearing away heat, dispelling wind, and detoxifying, and is clinically used to treat upper respiratory tract infections [1] . Research on the composition of Reduning Injection shows that it mainly contains caffeoylquinic acids and iridoids [2] . Previous research established the HPLC fingerprint of Reduning Injection [3] Determination of 9 Components in Reduning Injection with One Test and Multiple Evaluation Method [4] , the above-mentioned HPLC and one test and multiple evaluation methods run for a long time, which is not conducive to the rapid detection of products in the process of industrialized large-scale pro...
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