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Extraction method of berberine

A technology of Coptidis berberine and extraction method, which is applied in the direction of medical formula, medical preparations containing active ingredients, plant raw materials, etc., can solve the problems of prolonged time, poor effect, incomplete cell wall destruction, etc., and achieve shortened extraction time. Sufficient and yield-increasing effects

Inactive Publication Date: 2015-03-25
DINGZHENG ANIMAL PHARMA TIANJIN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After analysis, the main reason is that the extraction of effective substances must destroy the cell wall of the plant, while the destruction of the cell wall by traditional water extraction and alcohol extraction is not complete, resulting in the need to prolong the extraction time, use higher temperature and increase the number of auxiliary methods, but the effect still poor

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  • Extraction method of berberine

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Effect test

Embodiment 1

[0029] A method for extracting Coptidis berberine The innovation of the present invention lies in: comprising the following components:

[0030] ⑴ 1000 grams of Coptis chinensis is cleaned and dried, and the crushed powder is passed through a 30-mesh sieve for later use;

[0031] (2) Add the product of step (1) into the extraction tank, add 50% ethanol, the weight ratio of Coptidis rhizome powder and ethanol is 1:4, then add enzyme and acid to extract the active ingredient;

[0032] The enzyme is cellulase, and the consumption of said cellulase is 1% of the total weight of Coptis Rhizoma and ethanol;

[0033] The acid is dilute hydrochloric acid, the concentration of dilute hydrochloric acid is 9%, and the consumption is 0.5% of the total weight of Coptis Rhizoma and ethanol;

[0034] The extraction temperature is 40 degrees Celsius, and the extraction time is 1 hour. The filtered medicinal residues are added to the extraction tank, ethanol is added, enzymes and acids are add...

Embodiment 2

[0039] A method for extracting Coptidis berberine The innovation of the present invention lies in: comprising the following components:

[0040] ⑴ 1000 grams of Coptis chinensis is cleaned and dried, and the crushed powder is passed through a 40-mesh sieve for later use;

[0041] (2) Add the product of step (1) into the extraction tank, add 80% ethanol, the weight ratio of Coptidis rhizome powder and ethanol is 1:9, then add enzyme and acid to extract active ingredients;

[0042] The enzyme is cellulase, and the consumption of said cellulase is 1% of the total weight of Coptis Rhizoma and ethanol;

[0043] The acid is sulfuric acid, the concentration of sulfuric acid is 98%, and its consumption is 0.2% of the total weight of Coptis Rhizoma and ethanol;

[0044] The extraction temperature is 60 degrees Celsius, and the extraction time is 1 hour. The filtered medicinal residues are added to the extraction tank, ethanol is added, and enzymes and acids are added to extract active...

Embodiment 3

[0049] A method for extracting Coptidis berberine The innovation of the present invention lies in: comprising the following components:

[0050] (1) Wash 1000 grams of Coptis chinensis, dry it, pass the crushed powder through a 20-mesh sieve, and set aside;

[0051] (2) Add the product of step (1) into the extraction tank, add 70% ethanol, the weight ratio of Coptidis rhizome powder to ethanol is 1:6, then add enzyme and acid to extract active ingredients;

[0052] The enzyme is hemicellulase, and the dosage is 1.5% of the total weight of Coptis Rhizoma and ethanol

[0053] The acid is sulfuric acid, the concentration of sulfuric acid is 98%, and its consumption is 0.3% of the total weight of Coptis Rhizoma and ethanol;

[0054] The extraction temperature is 50 degrees Celsius, and the extraction time is 1 hour. The filtered medicinal residues are added to the extraction tank, ethanol is added, and enzymes and acids are added to extract active ingredients under the same tempe...

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Abstract

The invention relates to an extraction method of berberine. The extraction method of berberine comprises the following steps: (1) cleaning coptis chinensis and then airing, and sieving the crushed powder for later use; (2) adding the coptis chinensis powder in an extraction tank, adding ethyl alcohol, then adding an enzyme and an acid, and extracting effective ingredients; and (3) collecting the extract, carrying out reduced pressure concentration to recover ethyl alcohol until the relative density of the extract is 1.15-1.30, then preparing the finished product. In the extraction method of berberine disclosed by the invention, the enzyme is placed in the environment of acid and alcohol, breakages to plant cell walls are thorough, the extraction of the effective ingredients is more adequate, the reaction temperature is lowered by more than a half, and the time is shortened; the result of measuring the contents of berberine and chlorogenic acid in the concentrate by virtue of a high pressure liquid chromatography indicates that the yields of berberine and chlorogenic acid are greatly increased compared with a water extraction method and an alcohol extraction method, and the granules prepared from the finished product can be used for prevention and treatment of livestock and poultry viral diseases.

Description

technical field [0001] The invention belongs to the technical field of extracting effective components of Coptis chinensis, in particular to a method for extracting berberine from Coptis chinensis. Background technique [0002] Coptis chinensis is a perennial herb belonging to the genus Coptis genus of the family Ranunculaceae. Its rhizome is yellow and often branched, and its leaves are papery and ovate-shaped. Its flowering period is from February to April and its fruiting period is from March to June. It can be grown in a natural environment with low temperature and high air humidity. It is afraid of high temperature and drought, and cannot withstand strong sunlight. It is suitable for cultivation in soil with loose and fertile topsoil, rich humus, and deep soil layer. Coptis chinensis is one of the most widely traded Chinese herbal medicines, and also one of the rare and precious Chinese herbal medicines. It is a common Chinese medicine. Because its rhizome is bead-shape...

Claims

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Application Information

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IPC IPC(8): A61K36/718A61K31/4375A61K31/216A61P31/12
CPCA61K36/718A61K2236/333Y02A40/70
Inventor 刘鼎阔王立红赵云英赵晶晶张俊霞
Owner DINGZHENG ANIMAL PHARMA TIANJIN