Chinese soft-shell turtle aeromonas phage and application thereof
A technology of Aeromonas and Aeromonas hydrophila, applied in the field of microorganisms, can solve the problems of farmers using a large amount of drugs, and achieve the effects of small side effects, significant therapeutic effect, and convenient use
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[0051] Example 1:
[0052] A bacteriophage of Aeromonas sinensis is obtained as follows:
[0053] (1) Aeromonas was isolated from Chinese soft-shelled turtles suffering from neck disease, and fresh Aeromonas bacteria liquid was cultivated to make the bacteria liquid OD 550 Is 0.25;
[0054] (2) Add MgCl 2 Bring the final concentration to 4mM, let it stand for 20-30min, shake culture for 5 hours and then harvest;
[0055] (3) Transfer the culture solution into a centrifuge tube and centrifuge at 10,000 rpm for 10 minutes to precipitate the host bacteria;
[0056] (4) Transfer the supernatant to a new tube, and remove the residual bacteria with a 0.22μm pore filter to obtain the phage stock solution without host bacteria;
[0057] (5) To determine its potency by the double-layer plate method, the steps are as follows:
[0058] (A) Prepare 2% LB agar medium and 0.7% semi-solid LB medium, pour the melted 2% LB agar medium into the bottom of the petri dish, after solidification, become the bo...
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[0066] Example 2:
[0067] The obtaining steps and the treatment steps are basically the same as those in Example 1, except that the treatment object is a soft-shelled turtle with white spot disease.
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