Chinese soft-shell turtle aeromonas phage and application thereof

A technology of Aeromonas and Aeromonas hydrophila, applied in the field of microorganisms, can solve the problems of farmers using a large amount of drugs, and achieve the effects of small side effects, significant therapeutic effect, and convenient use

Inactive Publication Date: 2015-03-25
ZHEJIANG INST OF FRESH WATER FISHERIES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem of a variety of soft-shelled soft-shelled turtle diseases caused by Aeromonas sinensis disease in the breeding process, which leads to the large amount of medication used by farmers and the problems of food safety and ecological security, we proposed a

Method used

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  • Chinese soft-shell turtle aeromonas phage and application thereof
  • Chinese soft-shell turtle aeromonas phage and application thereof
  • Chinese soft-shell turtle aeromonas phage and application thereof

Examples

Experimental program
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Example Embodiment

[0051] Example 1:

[0052] A bacteriophage of Aeromonas sinensis is obtained as follows:

[0053] (1) Aeromonas was isolated from Chinese soft-shelled turtles suffering from neck disease, and fresh Aeromonas bacteria liquid was cultivated to make the bacteria liquid OD 550 Is 0.25;

[0054] (2) Add MgCl 2 Bring the final concentration to 4mM, let it stand for 20-30min, shake culture for 5 hours and then harvest;

[0055] (3) Transfer the culture solution into a centrifuge tube and centrifuge at 10,000 rpm for 10 minutes to precipitate the host bacteria;

[0056] (4) Transfer the supernatant to a new tube, and remove the residual bacteria with a 0.22μm pore filter to obtain the phage stock solution without host bacteria;

[0057] (5) To determine its potency by the double-layer plate method, the steps are as follows:

[0058] (A) Prepare 2% LB agar medium and 0.7% semi-solid LB medium, pour the melted 2% LB agar medium into the bottom of the petri dish, after solidification, become the bo...

Example Embodiment

[0066] Example 2:

[0067] The obtaining steps and the treatment steps are basically the same as those in Example 1, except that the treatment object is a soft-shelled turtle with white spot disease.

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Abstract

The invention provides a Chinese soft-shell turtle aeromonas phage of which the preservation number is CGMCC: No. 9623. Partial sequence fragments of the nucleic acid of the phage and the aeromonas phage are above 75% in homology, bacteriophage plaque can be formed for the Chinese soft-shell turtle aeromonas hydrophila, and a splitting action can be played on pathogenic aeromonas, so the Chinese soft-shell turtle aeromonas phage can be used for treating and preventing diseases caused by the aeromonas by adopting a biological treatment method. The Chinese soft-shell turtle aeromonas phage disclosed by the invention has the beneficial effects of being high in specificity, small in side effects, high in multiplication capacity, hard to cause resistance and pathogenic bacteria dependence, convenient to use and capable of having a remarkable treatment effect on Chinese soft-shell turtle neck sore, white-spot diseases and the like. By adopting the Chinese soft-shell turtle aeromonas phage disclosed by the invention to treat the Chinese soft-shell turtle neck sore, the white-spot diseases and the like, the aims of realizing high specificity, small side effects, high multiplication capacity, hard generation of resistance and pathogenic bacteria dependence and convenience in use can be fulfilled.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to an Aeromonas sinensis phage and its application. Background technique [0002] Chinese soft-shelled soft-shelled turtle (Trionyx sinensis) is the main species among the famous and high-quality products of freshwater aquaculture in my country. Diseases have always been an important limiting factor for the healthy and sustainable development of soft-shelled turtle aquaculture. Bacterial diseases are the main diseases of Chinese soft-shelled soft-shelled turtle. At present, it has been reported that Aeromonas, Edwardsiella, Proteus and other pathogenic bacteria can cause ascites disease, red floor disease, leukoplakia (spot) disease, red neck disease, rot skin perforation disease, white floor disease, gastrointestinal Inflammation, furunculosis, rotten tail (claw, neck) disease, etc. Among them, Aeromonas is the most common, which can cause intestinal hemorrhagic sepsis of soft-shelle...

Claims

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Application Information

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IPC IPC(8): C12N7/00A61P31/04C12R1/93
Inventor 刘莉曹铮许怡林锋叶雪平
Owner ZHEJIANG INST OF FRESH WATER FISHERIES
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