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Fermentation liquid for culturing Pleurotus eryngii substrate, medium for Pleurotus eryngii and preparation method thereof

A culture medium, the technology of king oyster mushroom, applied in the preparation and application of organic fertilizer, calcium fertilizer and other directions, can solve the problems of long time, uncontrolled product quality, etc. The effect of strong mycelium growth

Inactive Publication Date: 2017-06-16
XIHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, natural stacking fermentation is commonly used now, which takes a long time and the product quality is not controlled

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Preparation and Application of Cultivation Substrate Ⅰ

[0020] (1) Preparation of fermentation broth

[0021] Bacillus subtilis CMCC 63501 and Bacillus circulans ATCC 4513 were mixed with peptone 1% (W / V), NaCl 1% (W / V) and yeast powder 0.5% (W / V) in a 1:1 ratio. The LB liquid medium was cultured for 24 hours under the culture conditions of pH 7.0 and culture temperature of 37° C. to obtain a fermentation broth.

[0022] (2) Raw material ratio and mixing

[0023] The ratio of raw materials is: 20 parts by weight of cottonseed hulls, 10 parts by weight of wood chips, 12 parts by weight of corncobs, 28 parts by weight of bran, 15 parts by weight of bagasse, 13 parts by weight of bean stems, 10 parts by weight of rice bran, and 1 part by weight of calcium carbonate , 1 part by weight of lime.

[0024] The above raw materials are crushed into small granules the size of soybean grains, then mixed and evenly mixed, water is added to the material, the water content of the ...

Embodiment 2

[0033] Preparation and Application of Cultivation Substrate Ⅱ

[0034] (1) Preparation of fermentation broth

[0035] Bacillus subtilis and Bacillus circulans are in the LB liquid of peptone 1% (W / V)+NaCl1% (W / V)+yeast powder 0.5% (W / V) in the ratio of 3:2, pH is 7.2. Cultivate for 24 hours at a culture temperature of 37° C. to obtain a fermentation broth.

[0036] (2) Raw material ratio and mixing

[0037] The ratio of raw materials is: 40 parts by weight of cottonseed hulls, 20 parts by weight of wood chips, 20 parts by weight of corncobs, 15 parts by weight of bran, 10 parts by weight of bagasse, 8 parts by weight of bean stems, 5 parts by weight of rice bran, and 2 parts by weight of calcium carbonate , 2 parts by weight of lime.

[0038] The above-mentioned raw materials are crushed into small particles the size of soybean grains, then mixed and evenly mixed, water is added to the material, the water content of the culture material is adjusted to 66% by weight, and the...

Embodiment 3

[0047] Preparation and Application of Cultivation Substrate Ⅲ

[0048] (1) Preparation of fermentation broth

[0049] Put Bacillus subtilis and Bacillus circulans at a ratio of 6:5 in LB liquid of peptone 1% (W / V) + NaCl 1% (W / V) + yeast powder 0.5% (W / V), pH 6.8, the culture temperature is 36 ° C culture conditions for 23 hours to obtain fermentation broth.

[0050] (2) Raw material ratio and mixing

[0051] The ratio of raw materials is: 10 parts by weight of cottonseed hulls, 30 parts by weight of wood chips, 28 parts by weight of corn cobs, 10 parts by weight of bran, 5 parts by weight of bagasse, 5 parts by weight of bean stems, 15 parts by weight of rice bran, and 5 parts by weight of calcium carbonate , 4 parts by weight of lime.

[0052] The above-mentioned raw materials are crushed into small particles the size of soybean grains, then mixed and evenly mixed, water is added to the material, the water content of the culture material is adjusted to 65% by weight, and ...

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PUM

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Abstract

The invention relates to fermentation liquid for culturing pleurotus eryngii matrix, a pleurotus eryngii culture medium and a preparation method thereof, belonging to the technical field of edible fungi culture. The culture matrix is prepared from the following raw materials: cottonseed hull, sawdust, corncob, bran, bagasse, bean stalk, rice bran, calcium carbonate and quick lime; the raw materials are uniformly blended and stockpiled for fermentation together with the microbial fermentation liquid of bacillus subtilis and bacillus circulans; and the decomposition and transformation of organic matters such as lignin and cellulose can be accelerated by use of inoculated fermentation composting, and the nutrient substances such as carbohydrates and carbon nitride compounds required for the mycelial growth of pleurotus eryngii are directly supplied. The pleurotus eryngii culture medium is fully used, the fruiting time is shortened, the biological conversion rate is increased, and the investment cost is saved.

Description

technical field [0001] The invention relates to the technical field of edible fungus cultivation, in particular to a fermentation liquid for cultivating a pleurotus eryngii substrate, a pleurotus eryngii culture medium and a preparation method thereof. Background technique [0002] Pleurotus eryngii (Pleurotu seryngii), also known as Pleurotus eryngii, belongs to the phylum Fungi, Eubasidiomycetes Agaricaceae, and the genus Pleurotus. It is a precious fungus suitable for both medicine and food. Pleurotus eryngii has thick meat, crisp and tender texture, especially the white stipe, thick and long, extremely dense, firm, and strong crispness. It has a unique taste and is one of the best-tasting mushrooms. It is known as "Oyster Mushroom King", "Scallop Mushroom". Pleurotus eryngii is rich in nutrition, contains a variety of amino acids and trace elements, and has anti-oxidation, anti-virus and other active effects. It is a precious edible fungus with high nutritional value an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05G1/00C05F17/00
CPCC05D3/02C05F17/50C05F5/002C05F11/00C05F11/08Y02W30/40
Inventor 向文良蔡婷张庆唐洁林凯宋菲菲徐顾榕
Owner XIHUA UNIV
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