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Hyaluronidase and/or elastase inhibitor

A technology of hyaluronidase and elastase, which is applied in the direction of non-central analgesics, anti-inflammatory agents, allergic diseases, etc., and can solve the problems that have not yet been reported in the literature of isoquinoline alkaloids

Inactive Publication Date: 2015-04-08
何欣
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are no literature reports or patents about isoquinoline alkaloids as hyaluronidase or / and elastase inhibitors.

Method used

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  • Hyaluronidase and/or elastase inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1. Inhibition of isoquinoline alkaloids on elastase activity

[0021] Take the EP tube, add 80ul enzyme solution, add 450ul pure water, mix well, add 50ul prepared isoquinoline alkaloid solution, mix well, place in 37℃ water bath, incubate for 20 minutes. Then add 70ul elastase substrate and incubate for 90 minutes in a water bath at 37°C. Take it out, draw 200ul, measure the OD405 value, and calculate the inhibition rate (see Table 1).

Embodiment 2

[0022] Example 2. Isoquinoline alkaloids inhibit the activity of hyaluronidase

[0023] Take the EP tube, add 150ul buffer solution (acetic acid-sodium acetate, pH5.0), then add 100ul hyaluronidase solution, mix and add 20ul isoquinoline alkaloids of appropriate concentration and 80ul pure water, then add 150ul substrate Then incubate at 50°C for 2h. Take it out, add 100ul potassium ferricyanide solution and 100ul sodium bicarbonate solution, after boiling water bath for 5min, measure the OD405 value, and calculate the inhibition rate (see Table 1).

[0024] It can be seen from Table 1 that one or several isoquinoline alkaloids have different degrees of inhibitory effects on hyaluronidase and elastase.

[0025] Table 1. Inhibitory effects of different isoquinoline alkaloids on elastase and hyaluronidase

[0026]

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Abstract

The invention relates to a hyaluronidase inhibitor or / and an elastase inhibitor, and the hyaluronidase inhibitor or / and the elastase inhibitor is / are characterized by comprising isoquinoline alkaloids, and can be used for preparing drugs for treating hyaluronidase and / or elastase secretion increase related diseases by itself or by mixing with other traditional Chinese medicines or traditional Chinese medicine extracts or traditional Chinese medicine effective parts or chemical components or pharmaceutically acceptable carriers.

Description

technical field [0001] The invention relates to a hyaluronidase or / and elastase inhibitor, which is mainly one or several isoquinoline alkaloids. Background technique [0002] Hyaluronidase, also known as diffusion factor, is a hydrolase of hyaluronic acid, mainly found in the skin, joints, ligaments, and eyes, and participates in many pathophysiological activities of the human body, such as embryogenesis, angiogenesis, vascular reconstruction, Immune escape and tumor progression, and hyaluronidase is closely related to allergic and inflammatory responses. Therefore, hyaluronidase inhibitors have applications in many fields. For example, the patent CN 101304724 A utilizes the relationship between hyaluronidase and inflammation, and uses related inhibitors to relieve skin allergies. In recent years, hyaluronidase has been found to be closely related to a variety of inflammation-related diseases. For example, in the serum of patients with rheumatoid arthritis, the concentra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4375A61P29/00A61P37/08A61P11/02A61P19/02A61P35/00A61P1/00A61P17/00A61P1/04
CPCA61K31/4375A61K2300/00
Inventor 何欣
Owner 何欣