A method for constructing multifunctional gene therapy carrier and drug carrier based on silica nanoparticles
A nanoparticle and gene therapy technology, applied in gene therapy, pharmaceutical formulations, genetic material components, etc., can solve the problems of increased endocytosis, increased transfection efficiency, etc., to achieve easy regulation, simple use, good stability sexual effect
Active Publication Date: 2017-10-10
安徽泽升科技有限公司
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Problems solved by technology
[0011] 3. When preparing high-performance silica gene carriers, with the continuous grafting of monomers to the surface of silica nanoparticles, the molecular weight of cationic gene carriers increases, the endocytosis of cells increases, and the transfection efficiency increases, but The toxicity of cells also increases, how to minimize the toxicity of gene vectors becomes a problem to be solved
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Embodiment 1
[0033] 1) Preparation of amino-functionalized silica nanoparticles by silane coupling agent method: first mix 18mL ethanol and 2mL water evenly, then add 300μL 3-aminopropyltriethoxysilane and mix thoroughly, then add 200mg silica nanoparticles to disperse evenly Then react at room temperature for 6 hours, and finally add 160 μL triethylamine to continue the reaction at room temperature for 12 hours; wash the obtained product with 11,000 rpm centrifugal ethanol three times, dry it in vacuum, and cure at 130°C for 3 hours;
[0034] 2) Preparation of silica nanoparticle initiator: take 100 mg of amino-functionalized silica nanoparticles and dissolve in 12 mL CH 2 Cl 2 , then add 1.2mL of 2-bromoisobutyryl bromide and 1.2mL of triethylamine, react at room temperature for 3h, wash with methanol and water mixed solvent (methanol:water volume ratio is 1:1) at 11000rpm for three times, and vacuum dry;
[0035] 3) ATRP polymerization reaction: Dissolve 20mg of silica nanoparticle ini...
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The invention belongs to the technical field of preparation of non-viral gene carriers and drug carriers, and specifically relates to using silicon oxide nanoparticles with different shapes and sizes as substrates, utilizing surface modification and functionalizing silicon oxide nanoparticles, and using atom transfer radical polymerization (ATRP) method Build multifunctional vectors. The surface modification method adopted in the present invention has a mild polymerization reaction and is easy to control, and various multifunctional gene therapy carriers and drug carriers with different polymerization degrees can be prepared according to requirements, and the storage stability is good. The multifunctional gene therapy carrier and drug carrier have higher transfection efficiency than the international "gold standard" PEI in HepG2, COS7, 293, C6, Hela and other cells, and the cytotoxicity is far lower than that of polymer linear PDMAEMA. In addition, the shape of silica nanoparticles is easy to control, and special shapes and sizes can be designed according to different cell lines, and the porous structure of silica nanoparticles can be loaded with a variety of different drug molecules, laying a good foundation for the development of new genes / drugs Experimental basis.
Description
technical field [0001] The invention belongs to the technical field of non-viral gene carriers and drug carriers, and specifically relates to using silicon oxide nanoparticles as substrates, utilizing surface-modified functionalized silicon oxide nanoparticles, and using atom transfer radical polymerization (ATRP) to construct multifunctional gene therapy carriers and Drug carrier method. Background technique [0002] With the increasing incidence of cancer and other genetic diseases, the application of gene therapy in the treatment of cancer and other diseases has attracted widespread attention. Gene therapy refers to the introduction of exogenous normal genes into target cells to correct or compensate for diseases caused by gene defects and abnormalities, so as to achieve the purpose of treatment, that is, to insert exogenous genes into appropriate cells of patients through gene transfer technology. In recipient cells, the products made by exogenous genes can treat certai...
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IPC IPC(8): A61K47/32A61K47/04A61K48/00C08F292/00
Inventor 徐福建赵娜娜林心怡
Owner 安徽泽升科技有限公司