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Preparation method and application of silkworm model of hyperproteinemia

A technology of hyperproteinemia and silkworm, which is applied in the field of medicine, can solve the problems of costing a lot of money, hindering foreign cooperative research and trade of pharmaceutical products, and achieves the advantages of simple operation, increased rate of dysplasia and mortality, and improved metabolic activity. Effect

Inactive Publication Date: 2015-04-29
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since mammals are used as disease models of metabolism in the body, large-scale metabolic testing in the initial drug screening stage requires a lot of money and a series of ethical issues, which has become an important reason for restricting drug development in developed countries and has become an obstacle my country's Barriers to Foreign Cooperative Research and Pharmaceutical Products Trade

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  • Preparation method and application of silkworm model of hyperproteinemia
  • Preparation method and application of silkworm model of hyperproteinemia
  • Preparation method and application of silkworm model of hyperproteinemia

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preparation example Construction

[0026] A method for preparing a silkworm model of hyperproteinemia. After the silkworm larvae mature and stop eating or during silk spinning process, destroy or block the silk spinning holes, and continue feeding for 36 hours to obtain a silkworm model of hyperproteinemia.

[0027] In the preparation method of the silkworm model of hyperproteinemia of the present invention, the silkworm can use normal silkworms, and can also use other disease models, behavioral genetic models and growth and development model mutant strains to construct dual-disease or multiple-disease models in combination.

[0028] The method for destroying silkworm silkworm silkworm model described in the preparation method of the hyperproteinemia silkworm model of the present invention can adopt any method that damages silkworm silkworm silkworm and prevents larvae from spinning out silk protein.

[0029] In some embodiments, the method of destroying the spinning hole is high temperature burn or laser burn. ...

Embodiment 1

[0046] Embodiment 1: The establishment of the silkworm hyperproteinemia disease model of plugging silk-spinning holes

[0047] The silk-spinning pores of 100 silkworm larvae were blocked with low-melting point paraffin to prevent the silkworm from secreting silk protein, and to obtain a hyperproteinemia disease model silkworm. The control silkworm of the silkworm disease model is the same batch of silkworms that normally weave silk and cocoon.

[0048] The specific operation is carried out according to the following steps:

[0049] (1) Dissolve the paraffin with a melting point of 48-52°C and keep the temperature of the liquid paraffin not exceeding 55°C.

[0050] (2) Immediately take out the head front end of the mature larvae that has stopped eating after being immersed in the paraffin liquid quickly to form a paraffin layer to prevent the silkworm from spinning. The front end of the head of the mature larva refers to the front end where the silk-spinning hole tissue accou...

Embodiment 2

[0053] Example 2: The establishment of the silkworm hyperproteinemia disease model of high temperature scald silk hole

[0054] The silk-spinning holes of 100 silkworm larvae were quickly scalded with electric heating wire to prevent the silkworm from secreting silk protein, and obtain the hyperproteinemia disease model silkworm. The control silkworm of the silkworm disease model is the same batch of silkworms that normally weave silk and cocoon.

[0055] The specific operation is carried out according to the following steps:

[0056](1) Heating a heating wire with a diameter of less than 100nm to a temperature of 150-350°C.

[0057] (2) Rapidly scald the silk-spinning holes of the larvae 12 hours after the start of silk-spinning, forming stickiness between the silk-spinning holes and surrounding tissues, preventing the silkworm from spinning.

[0058] (3) Protect the environment at 25°C, 70%-85% humidity, and natural light to obtain a model silkworm with moderate symptoms o...

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Abstract

The invention belongs to the field of medicine, and discloses a preparation method and an application of a silkworm model of hyperproteinemia. The preparation method of an animial model of the hyperproteinemia comprises the steps of destroying or blocking a spinning hole when silkworm larvae is mature and stop feed or in the spinning process of the silkworm larvae, standing and protecting for 36h to obtain the silkworm model of the hyperproteinemia. The preparation method of the silkworm model of the hyperproteinemia is simple to operate, the silkworm model of the hyperproteinemia with the highest level of total proteins in circulating blood of 100 to 350 percent of a normal reference animal can be rapidly acquired, and the animal death rate is freely controlled within the range of no individual death to 100 percent death. The silkworm model of the hyperproteinemia is slow and abnormal in tissue degradation and tissue rebuilding process, the development abnormality rate and death rate are remarkably improved, and the method can be used for screening a drug overcoming the development abnormality and lethality at the later stage and for screening the drug for improving the metabolic activity of the proteins of the circulating blood.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a preparation method and application of a silkworm model of hyperalbuminemia. Background technique [0002] Protein metabolism is based on amino acids. Food protein must first be degraded into amino acids before it can be used by the body, and protein in the body must first be decomposed into amino acids before it can continue to be oxidized and decomposed or transformed. Free amino acids can synthesize their own proteins, can be oxidized and decomposed to release energy, can be converted into sugars or lipids, and can also synthesize other biologically active substances. All the free amino acids in the extracellular and extracellular fluids are called the free amino acid pool. Although its content is less than 1% of the total amino acids in the body, it can reflect the general situation of the body's nitrogen metabolism. Circulating blood protein and amino acid levels refle...

Claims

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Application Information

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IPC IPC(8): A01K67/04G01N33/68
CPCA01K67/04A01K2267/03
Inventor 徐世清陈学东王璐王欢司马杨虎殷为民陶卉邢瑞刘安琪鄢思琪王玉龙
Owner SUZHOU UNIV